In vitro shoot growth of Brugmansia × candida Pers.

The objective of this study was to improve the growth of in vitro shoot cultures of Brugmansia × candida 'Creamsickle'. Several mineral nutrient experiments were conducted to determine the effect of NH4 (+), NO3 (-), K(+), FeSO4/EDTA, ZnSO4, MnSO4, and CuSO4 on quality, leaf width and length, size and weight of shoot mass, and shoot number. The experiment to determine the levels of NH4 (+), NO3 (-), and K(+), was conducted as a 2-component NH4 (+): K(+) mixture crossed by [NO3 (-)] and resulted in an experimental design free of ion confounding and capable of separating the effects of proportion and concentration.