CD99 Modulates the Proteomic Landscape of Ewing Sarcoma Cells and Related Extracellular Vesicles.

Ewing sarcoma (EWS) is an aggressive pediatric bone tumor characterized by unmet clinical needs and an incompletely understood epigenetic heterogeneity. Here, we considered CD99, a major surface molecule hallmark of EWS malignancy. Fluctuations in CD99 expression strongly impair cell dissemination, differentiation, and death.

EVALUATING CONTRADICTORY EXPERIMENTAL AND NON-EXPERIMENTAL ESTIMATES OF NEIGHBORHOOD EFFECTS ON ECONOMIC OUTCOMES FOR ADULTS.

Although non-experimental studies find robust neighborhood effects on adults, such findings have been challenged by results from the Moving to Opportunity (MTO) residential mobility experiment. Using a within-study comparison design, this paper compares experimental and non-experimental estimates from MTO and a parallel analysis of the Panel Study of Income Dynamics (PSID). Striking similarities were found between non-experimental estimates based on MTO and PSID.

Trophic niche influences ingestion of micro- and mesoplastics in pelagic and demersal fish from the Western Mediterranean Sea.

Plastic pollution has been extensively documented in the marine food web, but targeted studies focusing on the relationship between microplastic ingestion and fish trophic niches are still limited. In this study we investigated the frequency of occurrence and the abundance of micro- and mesoplastics (MMPs) in eight fish species with different feeding habits from the western Mediterranean Sea. Stable isotope analysis (δC and δN) was used to describe the trophic niche and its metrics for each species.

Bib Sign in Proximal Descending Thoracic Aorta Rupture on CT Angiography: Presentation of a Paradigmatic Case.

Thoracic aortic rupture may present with subtle clinical and CT-angiography findings. Recognition of the imaging features of early rupture is key for timely diagnosis and treatment. This report presents a new sign of incipient proximal thoracic aortic rupture on CT-angiography.

Whole-Body Low-Dose Multidetector-Row CT in Multiple Myeloma: Guidance in Performing, Observing, and Interpreting the Imaging Findings.

Multiple myeloma is a hematological malignancy of plasma cells usually detected due to various bone abnormalities on imaging and rare extraosseous abnormalities. The traditional approach for disease detection was based on plain radiographs, showing typical lytic lesions. Still, this technique has many limitations in terms of diagnosis and assessment of response to treatment.

A model-based approach to Spotify data analysis: a Beta GLMM.

Digital music distribution is increasingly powered by automated mechanisms that continuously capture, sort and analyze large amounts of Web-based data. This paper deals with the management of songs audio features from a statistical point of view. In particular, it explores the data catching mechanisms enabled by Spotify Web API and suggests statistical tools for the analysis of these data.

Resilience of the seagrass Posidonia oceanica following pulse-type disturbance.

Understanding the response of species to disturbance and the ability to recover is crucial for preventing their potential collapse and ecosystem phase shifts. Explosive submarine activity, occurring in shallow volcanic vents, can be considered as a natural pulse disturbance, due to its suddenness and high intensity, potentially affecting nearby species and ecosystems. Here, we present the response of Posidonia oceanica, a long-lived seagrass, to an exceptional submarine volcanic explosion, which occurred in the Aeolian Archipelago (Italy, Mediterranean Sea) in 2002, and evaluate its resilience in terms of time required to recover after such a pulse event.

GAMLSS for high-variability data: an application to liver fibrosis case.

This article aims to provide rigorous and convenient statistical models for dealing with high-variability phenomena. The presence of discrepance in variance represents a substantial issue when it is not possible to reduce variability before analysing the data, leading to the possibility to estimate an inadequate model. In this paper, the application of Generalized Additive Model for Location, Scale and Shape (GAMLSS) and the use of finite mixture model for GAMLSS will be proposed as a solution to the problem of overdispersion.

Dimethyl fumarate vs Teriflunomide: an Italian time-to-event data analysis.

Background: The introduction of oral disease-modifying therapies (DMTs) for relapsing-remitting multiple sclerosis (RRMS) changed algorithms of RRMS treatment.

Objectives: To compare the effectiveness of treatment with dimethyl fumarate (DMF) and teriflunomide (TRF) in a large multicentre Italian cohort of RRMS patients.

Materials And Methods: Patients with RRMS who received treatment with DMF and TRF between January 1st, 2012 and December 31st, 2018 from twelve MS centers were identified.

Circulating miR34a levels as a potential biomarker in the follow-up of Ewing sarcoma.

Appropriate tools for monitoring sarcoma progression are still limited. The aim of the present study was to investigate the value of miR-34a-5p (miR34a) as a circulating biomarker to follow disease progression and measure the therapeutic response. Stable forced re-expression of miR34a in Ewing sarcoma (EWS) cells significantly limited tumor growth in mice.

A housing mobility program's impacts on teen and young adult parenting.

Objective: To assess the impact of Moving to Opportunity for Fair Housing Demonstration Program (MTO) implemented in 1994 in five U.S. cities (Baltimore, Boston, Chicago, Los Angeles, and New York City) on teen births.

Exosomes from CD99-deprived Ewing sarcoma cells reverse tumor malignancy by inhibiting cell migration and promoting neural differentiation.

Ewing sarcoma (EWS) is an aggressive mesenchymal tumor with unmet clinical need and significant social impacts on children, adolescents, and young adults. CD99, a hallmark surface molecule of EWS, participates in crucial biological processes including cell migration, differentiation, and death. EWS cells can release CD99 through exosomes (EXOs), specialized extracellular vesicles with major cell communication roles.

Discontinuation of teriflunomide and dimethyl fumarate in a large Italian multicentre population: a 24-month real-world experience.

Background: Teriflunomide (TRF) and Dimethyl fumarate (DMF) are licensed drugs for relapsing-remitting Multiple Sclerosis (RRMS).

Objectives: We aimed to compare the rate and the time to discontinuation among persons with RRMS (pwRRMS), newly treated with TRF and DMF.

Materials And Methods: A retrospective study on prospectively collected data was performed in nine tertiary MS centers, in Italy.

A simple UHPLC-PDA method with a fast dilute-and-shot sample preparation for the quantification of canrenone and its prodrug spironolactone in human urine samples.

Introduction: Nowadays, the treatment of hypertension represents an important issue, particularly in developed countries. While in most cases the standard therapeutic approaches, consisting in the administration of 1 to 3 drugs, are adequate to reach adequate blood pressure levels, in some cases more drugs are needed: this condition is called "resistant hypertension". In this context, the administration of a diuretic, such as spironolactone or canrenoate salts, represents a standard practice.

Clofarabine inhibits Ewing sarcoma growth through a novel molecular mechanism involving direct binding to CD99.

Ewing sarcoma (ES) is an aggressive bone and soft tissue malignancy that predominantly affects children and adolescents. CD99 is a cell surface protein that is highly expressed on ES cells and is required to maintain their malignancy. We screened small molecule libraries for binding to extracellular domain of recombinant CD99 and subsequent inhibition of ES cell growth.

UHPLC-MS/MS method with sample dilution to test therapeutic adherence through quantification of ten antihypertensive drugs in urine samples.

Nowadays, hypertension represents an important health problem, particularly in developed countries. In some cases the standard therapeutic approaches are not able to reestablish the normal blood pressure values: this condition is called "resistant hypertension". However, a fraction of cases of resistant hypertension are actually due to poor adherence to the prescribed therapy.

Modelling tumour volume variations in head and neck cancer: contribution of magnetic resonance imaging for patients undergoing induction chemotherapy.

Primary tumour volume evaluation has predictive value for estimating survival outcomes. Using volumetric data acquired by MRI in patients undergoing induction chemotherapy (IC) these outcomes were estimated before the radiotherapy course in head and neck cancer (HNC) patients. MRI performed before and after IC in 36 locally advanced HNC patients were analysed to measure primary tumour volume.

CD99 triggering induces methuosis of Ewing sarcoma cells through IGF-1R/RAS/Rac1 signaling.

CD99 is a cell surface molecule that has emerged as a novel target for Ewing sarcoma (EWS), an aggressive pediatric bone cancer. This report provides the first evidence of methuosis in EWS, a non-apoptotic form of cell death induced by an antibody directed against the CD99 molecule. Upon mAb triggering, CD99 induces an IGF-1R/RAS/Rac1 complex, which is internalized into RAB5-positive endocytic vacuoles.

Warming-related shifts in the distribution of two competing coastal wrasses.

Warming induces organisms to adapt or to move to track thermal optima, driving novel interspecific interactions or altering pre-existing ones. We investigated how rising temperatures can affect the distribution of two antagonist Mediterranean wrasses: the 'warm-water' Thalassoma pavo and the 'cool-water' Coris julis. Using field surveys and an extensive database of depth-related patterns of distribution of wrasses across 346 sites, last-decade and projected patterns of distribution for the middle (2040-2059) and the end of century (2080-2099) were analysed by a multivariate model-based framework.

An Overview of Diagnosis of Primary Autoimmune Hypophysitis in a Prospective Single-Center Experience.

Background: Autoimmune hypophysitis is a rare disease with a natural progression that is not well known.

Aim: To collect representative data on clinical features of autoimmune hypophysitis and better characterize the disease.

Patients And Methods: A prospective single-center study was designed.

Differential diagnosis of parotid gland tumours: which magnetic resonance findings should be taken in account?

Our aim was to define typical magnetic resonance (MRI) findings in malignant and benign parotid tumours. This study is based on retrospective evaluation of pre-surgical MRI of 94 patients with parotid gland tumours. Histology results were available for all tumours.

Neighborhood effects on use of African-American Vernacular English.

African-American Vernacular English (AAVE) is systematic, rooted in history, and important as an identity marker and expressive resource for its speakers. In these respects, it resembles other vernacular or nonstandard varieties, like Cockney or Appalachian English. But like them, AAVE can trigger discrimination in the workplace, housing market, and schools.

Preliminary Results in Unresectable Head and Neck Cancer Treated by Radiofrequency and Microwave Ablation: Feasibility, Efficacy, and Safety.

Purpose: To retrospectively determine whether the application of thermal ablation to recurrent and advanced head and neck cancer (HNC) could allow for local tumor control.

Materials And Methods: From 2002 to 2014, 22 patients (17 men and 5 women; mean age, 64 y; age range, 42-88 y) with unresectable HNC lesions treated with thermal ablation were evaluated. Patients were followed at 3 and 6 months after treatment, every 6 months for 5 years, and yearly thereafter with computed tomography and/or magnetic resonance imaging.

Integrated approaches to miRNAs target definition: time-series analysis in an osteosarcoma differentiative model.

Background: microRNAs (miRs) are small non-coding RNAs involved in the fine regulation of several cellular processes by inhibiting their target genes at post-transcriptional level. Osteosarcoma (OS) is a tumor thought to be related to a molecular blockade of the normal process of osteoblast differentiation. The current paper explores temporal transcriptional modifications comparing an osteosarcoma cell line, Saos-2, and clones stably transfected with CD99, a molecule which was found to drive OS cells to terminally differentiate.