Thermostable WW-Domain Scaffold to Design Functional β-Sheet Miniproteins.

There has been a recent surge in the design of miniproteins for medicinal chemistry, biomaterial design, or synthetic biology. In particular, there is an interest in peptide scaffolds that fold reliably, predictably, and with solid stability. In this article, we present the design of a highly thermostable WW domain, a three-stranded β-sheet motif, with a superior melting temperature of about 90 °C to serve as a core scaffold onto which receptor-like properties can be grafted.

Multimaterial 3D Laser Printing of Cell-Adhesive and Cell-Repellent Hydrogels.

Here, a straightforward method is reported for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability, and rapid and precise 3D fabrication. The printable cell-adhesive polyethylene glycol (PEG) based material includes an Arg-Gly-Asp (RGD) containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design.

Trapping and Ground-State Cooling of a Single H_{2}^{+}.

We demonstrate co-trapping and sideband cooling of a H_{2}^{+}-^{9}Be^{+} ion pair in a cryogenic Paul trap. We study the chemical lifetime of H_{2}^{+} and its dependence on the apparatus temperature, achieving lifetimes of up to 11_{-3}^{+6}  h at 10 K. We demonstrate cooling of two of the modes of translational motion to an average phonon number of 0.

Effect of timing of surgery on survival after preoperative hyperfractionated accelerated radiotherapy (HART) for locally advanced rectal cancer (LARC): is it a matter of days?

We intend to analyse retrospectively whether the time interval ("gap duration" = GD) between preoperative radiotherapy and surgery in locally advanced rectal cancer (LARC) has an impact on overall survival (OS), cancer specific survival (CSS), disease free survival (DFS) and local control (LC). Two hundred seventy nine patients with LARC were entered in Trial 93-01 (hyperfractionated accelerated radiotherapy 41.6 Gy/26 Fx BID) shortly followed by surgery.

[Correlation of risk factors with the efficacy of applied therapies by breast conserving procedure in breast cancer].

1007 cases of female breast cancer patients treated with breast conserving surgery and subsequently irradiation with a median dose of 66 (50-80) Gy including boost with tangential high voltage photon beams. 34.6% (348/1007) received no further therapies, 53.

[Breast carcinoma: value of adjuvant therapy after mastectomy? Overview of personal results over > or = 20 years].

Analysis of 626 consecutive locoregional postoperative irradiated patients after mastectomy. 49.5% (310/626) were without further therapies, 32.

[Inoperable non-small-cell bronchial carcinoma: macroscopic and microscopic tumor behavior during and after radiotherapy with curative intent].

The supervision of the efficacy of therapy with curative aim in inoperable NSCLC focus on clinical and radiological parameters and the survival rate. But the decision about the local tumour elimination lies in the microscopic area, which cannot be controlled neither by laboratory tests nor by radiological examinations. About twenty years ago with support of our pneumologist we carried out bronchoscopies and biopsies depending on the applied radiation dose.

[Breast-preserving therapy: 19 years of monoinstitutional experience and results].

After conservative surgery 491 women with unilateral, invasive breast cancer were irradiated with a median dose of 50 Gy (ICRU-point) for the whole breast and an additional boost of 20 Gy respectively. The mean (median) follow-up was 69.7 (60) months with a range from 24 to 221 months.

[The gonadal loading during the irradiation of lymph outflow in operated seminomas. In-vivo dosimetry].

Background: This article should demonstrate the problems concerning gonadal dose in seminoma patients, the impact of shielding and possible consequences for therapy and advising of patients with desire to have children.

Patients And Method: Since November 1993 gonadal doses of 43 patients (Stage I/II, Royal Marsden) have been determined in 80 measurements with 2 ionization chambers on the ipsi- and contralateral side of the remaining testicle. The patients were all treated with ap/pa "hockey-stick"-shaped fields on a 6 MV linear accelerator.

[Lung cancer--hopelessness in inoperability? A 10-year follow-up].

Background: According to reports of Durrant et al. [19] and Berry et al. [5] it was concluded that non-operable non-small cell lung tumors cannot be cured.

[Local efficiency of percutaneous radiotherapy in lung cancer. Analysis of 215 repeated bronchoscopies in relation to applied radiation dosage].

Purpose: During a locoregional radiotherapy with curative attempts of lung cancer patients bronchoscopic examinations with biopsies and/or cytologic lavages were repeated to assess the accuracy of limiting the total dose to 60 Gy. In order of the applied dose macroscopic changements of the endoluminal tumor and microscopic elimination should be made out. The correlation between macro- and microscopical regression should allow a statement about reliability of single results.

[Breast-preserving treatment of breast carcinoma: analysis of surgical, pathologic-anatomic and radiotherapeutic data of 291 cases].

291 breast cancer patients treated with conserving surgery and subsequent locoregional irradiation were studied. The method of surgery consisted of 200 lumpectomies, 76 quadrantectomies and 15 with either a wide excision or with an atypical resection. In at least 42.

[Radiotherapy of malignant lymphomas of the brain: Radiologic behavior in relation to radiation dose and volume].

Purpose: During the radiotherapy of malignant CNS-lymphomas computertomographic controls were performed as a routine. The radiologic results should give a base to define the total dose. The aim was to determine the value of such individual parameters.

[Endoscopic, biopsy and functional results in irradiated esophageal carcinoma].

83 patients of average age 64 (36-86) years with esophageal cancer were irradiated according to a prospectively defined treatment concept. After 60 Gy, re-endoscopy with biopsy was scheduled. If the tumor was still macro- or microscopically described, a boost to 70 Gy was given.

[Hyperthermia--a new element in cancer treatment].

The efficacy of radiation therapy combined with local hyperthermia is demonstrated by three case studies. Mode of action and problems are discussed and published results reported. Human tumour cells obtained of biopsies from our patients before the onset of treatments were investigated.

[Radiotherapy of esophageal carcinoma. Results checked clinically by radiology, endoscopy, biopsy and autopsy].

Forty-four patients with histologically confirmed esophageal cancer were irradiated with 60 Gy, checked endoscopically and by biopsy, and then followed clinically or radiologically until their death. The findings confirm the local destructibility of esophageal cancer with loosely ionized radiation and a favorable effect on quality of life. Three quarters of the patients were able to take normal food at the end of the radiotherapy, while the remainder were at least able to swallow soft foods.