Three-Dimensional Planning for Vascularized Bone Grafts: Implementation and Surgical Application for Complex Bone Reconstruction in the Hand and Forearm.

Vascularized bone grafts have been successfully established for complex bone defects. The integration of three-dimensional (3D) simulation and printing technology may aid in more precise surgical planning and intraoperative bone shaping. The purpose of the present study was to describe the implementation and surgical application of this innovative technology for bone reconstruction.

3D Technology for Autologous Breast Reconstruction: Increased Aesthetic Outcomes and Quality of Life Using Patient-Specific Templates.

Background: In autologous breast reconstruction accomplishing aesthetically pleasing outcomes represents an integral challenge. 3-dimensional technology may aid in accurate flap shaping and subsequent breast appearance.

Objectives: The aim of this study was to evaluate the applicability of 3-dimensional technology for surgical planning and its influence on outcomes for breast reconstruction.

Increased basal fibronectin is sufficient to promote excess endothelial cell matrix assembly causing localized barrier dysfunction.

Endothelial cell behavior is regulated by subendothelial extracellular matrix (ECM). The ECM protein fibronectin (FN) is rare in healthy blood vessels but accumulates in disease accompanied by endothelial dysfunctions. Here, we report that excess assembly of FN disrupts key endothelial functions.

Identification of a fibronectin-binding protein signature associated with idiopathic pulmonary fibrosis.

The extracellular matrix (ECM) is a critical component of tissue where it provides structural and signaling support to cells. Its dysregulation and accumulation lead to fibrosis, a major clinical challenge underlying many diseases that currently has little effective treatment. An understanding of the key molecular initiators of fibrosis would be both diagnostically useful and provide potential targets for therapeutics.

Simultaneous identification and quantification of three water-soluble polymers (PVP, PNVCL and PEI) in wastewater samples by continuous-flow off-line pyrolysis GC/MS.

In environmental analysis, the detection of water-soluble synthetic polymers (WSSP) presents considerable challenges. Thus, a precise and reproducible analytical method was developed using continuous-flow off-line pyrolysis with gas chromatography/mass spectrometry (GC/MS) to simultaneously identify multiple water-soluble polymers from a single environmental sample. WSSP are widely used in multiple industries as hydrogels due to their hydrophilic character and potential biocompatibility.

Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons.

The composition of the extracellular matrix (ECM) in nervous tissue plays an important role in controlling neuronal outgrowth and synapse development. Changes in both protein and glycosaminoglycan components of the ECM occur with tissue injury and may affect neuron growth. To investigate neuron responses to alterations in fibronectin (FN), a major component of the wound ECM, we grew cortical neurons on cell-derived decellularized matrices composed of wild type FN (FN) or of a mutant form of FN (FN) from which the III heparin-binding site had been deleted by CRISPR-Cas 9 gene editing.

Open-Spaced Ridged Hydrogel Scaffolds Containing TiO-Self-Assembled Monolayer of Phosphonates Promote Regeneration and Recovery Following Spinal Cord Injury.

The spinal cord has a poor ability to regenerate after an injury, which may be due to cell loss, cyst formation, inflammation, and scarring. A promising approach to treating a spinal cord injury (SCI) is the use of biomaterials. We have developed a novel hydrogel scaffold fabricated from oligo(poly(ethylene glycol) fumarate) (OPF) as a 0.

Nucleation of fibronectin fibril assembly requires binding between heparin and the 13th type III module of fibronectin.

Fibronectin (FN), a critical component of the extracellular matrix, is assembled into fibrils through a cell-mediated process. Heparan sulfate (HS) binds to the III module of FN, and fibroblasts lacking this glycosaminoglycan exhibit reduced FN fibril assembly. To determine if HS depends on III to control FN assembly, we deleted both III alleles in NIH 3T3 cells using the CRISPR-Cas9 system.

A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy.

Fibronectin (Fn1) fibrils have long been viewed as continuous fibers composed of extended, periodically aligned Fn1 molecules. However, our live-imaging and single-molecule localization microscopy data are inconsistent with this traditional view and show that Fn1 fibrils are composed of roughly spherical nanodomains containing six to eleven Fn1 dimers. As they move toward the cell center, Fn1 nanodomains become organized into linear arrays, in which nanodomains are spaced with an average periodicity of 105±17 nm.

Suitable indicators to determine tsunami impact on coastal areas in Northern Japan, Aomori Prefecture.

The 2011 Tohoku-oki tsunami released and mobilized many anthropogenic and natural organic compounds and, hereby, left a clear signature in its sedimentary remnants. In this study, a wide variety of organic marker substances were analyzed in 15 sediment profiles from the Aomori coast (Northern Japan). Total organic carbon (TOC) and fine grain fraction normalization have been tested with the wide dataset, and the already more frequently used TOC normalization was proven to be the more suitable one.

Implications of fibrotic extracellular matrix in diabetic retinopathy.

Fibrosis is an accumulation of extracellular matrix (ECM) proteins and fibers in a disordered fashion, which compromises cell and tissue functions. High glucose-induced fibrosis, a major pathophysiological change of diabetic retinopathy (DR), severely affects vision by compromising the retinal vasculature and ultimately disrupting retinal tissue organization. The retina is a highly vascularized, stratified tissue with multiple cell types organized into distinct layers.

Contemporary pollution of surface sediments from the Algarve shelf, Portugal.

The present-day human footprint is traceable in all environments. Growing urban centers, tourism, agricultural and industrial activities in combination with fishery, aquacultures and intense naval traffic, result in a large output of pollutants onto coastal regions. The Algarve shelf (Portugal) is one exemplary highly affected coastal system.

Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites.

Fibronectin (FN), an essential component of the extracellular matrix (ECM), is assembled via a cell-mediated process in which integrin receptors bind secreted FN and mediate its polymerization into fibrils that extend between cells, ultimately forming an insoluble matrix. Our previous work using mutant Chinese hamster ovary (CHO) cells identified the glycosaminoglycan heparan sulfate (HS) and its binding to FN as essential for the formation of insoluble FN fibrils. In this study, we investigated the contributions of HS at an early stage of the assembly process using knockdown of exostosin-1 (EXT1), one of the glycosyltransferases required for HS chain synthesis.

Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.

The extracellular matrix (ECM) plays a critical role in development, homeostasis, and regeneration of tissue structures and functions. Cell interactions with the ECM are dynamic and cells respond to ECM remodeling by changes in morphology and motility. During nerve regeneration, the ECM facilitates neurite outgrowth and guides axons with target specificity.

Remobilization of pollutants during extreme flood events poses severe risks to human and environmental health.

While it is well recognized that the frequency and intensity of flood events are increasing worldwide, the environmental, economic, and societal consequences of remobilization and distribution of pollutants during flood events are not widely recognized. Loss of life, damage to infrastructure, and monetary cleanup costs associated with floods are important direct effects. However, there is a lack of attention towards the indirect effects of pollutants that are remobilized and redistributed during such catastrophic flood events, particularly considering the known toxic effects of substances present in flood-prone areas.

The Proteome of Preretinal Tissue in Proliferative Vitreoretinopathy.

Background And Objective: Proliferative vitreoretinopathy (PVR) is the leading cause of retinal detachment repair failure. However, the molecular pathogenesis remains incompletely understood. Determining the proteome of PVR will help to identify novel therapeutic targets.

Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins.

Spinal cord injury (SCI) results in cell death, demyelination, and axonal loss. The spinal cord has a limited ability to regenerate, and current clinical therapies for SCI are not effective in helping promote neurologic recovery. We have developed a novel scaffold biomaterial that is fabricated from the biodegradable hydrogel oligo(poly(ethylene glycol)fumarate) (OPF).

Tracing woody-organic tsunami deposits of the 2011 Tohoku-oki event in Misawa (Japan).

With a minimum of three reported waves, the 2011 Tohoku-oki tsunami's destructive force caused massive damage along the northern Japanese Aomori coast. At Misawa the coastal control area was inundated up to 550 m inland and sandy sediment remnants can be traced to c. 350 m (c.

Environmental Earth Sciences Progress Report 2020 and Outlook 2021.

The present editorial 2020 continues the series of status reports in Environmental Earth Sciences (EES) in previous years 2017 and 2019 (Kolditz et al. in Environ Earth Sci 77: 8, 2018, Kolditz et al. in Environ Earth Sci 79: 11, 2020).

Fibronectin fibril alignment is established upon initiation of extracellular matrix assembly.

The physical structure of the extracellular matrix (ECM) is tissue-specific and fundamental to normal tissue function. Proper alignment of ECM fibers is essential for the functioning of a variety of tissues. While matrix assembly in general has been intensively investigated, little is known about the mechanisms required for formation of aligned ECM fibrils.

Application of multi-step approach for comprehensive identification of microplastic particles in diverse sediment samples.

The tremendous increase of plastic production, its intensive usage in packaging, as transport material, and the insufficient management of plastic garbage have led to a rise in microplastic particles as an anthropogenic contaminant in our environment. To develop appropriate management and remediation strategies for this global pollution problem, reliable and consistent analytical procedures for measuring plastics in the complex matrices need to be designed. The applicability of an easy, robust and fast multi-step approach was tested on three sediment samples from riverine, beach and backwater areas of varying origin, grain size and organic matter content, and is reported here.

Elevated glucose alters global gene expression and tenascin-C alternative splicing in mesangial cells.

Mesangial cells are the major extracellular matrix (ECM)-producing cells in the kidney glomerulus and, when exposed to elevated glucose levels, they up-regulate assembly of fibronectin (FN) and other ECM proteins. Increases in glucose concentration are known to alter gene expression; here we investigated the connection between increased ECM production and changes in gene expression in mesangial cells. Comparison of mesangial cells grown in normal or high glucose conditions by RNA-sequencing showed significant expression changes in over 6000 genes and, when grouped by KEGG pathway analysis, identified the ECM-receptor interaction and focal adhesion pathways among the top 5 upregulated pathways.

First insights into the formation and long-term dynamic behaviors of nonextractable perfluorooctanesulfonate and its alternative 6:2 chlorinated polyfluorinated ether sulfonate residues in a silty clay soil.

Per- and polyfluoroalkyl substances (PFAS) are persistent and toxic contaminants that are ubiquitous in the environment. They can incorporate into soil as nonextractable residues (NER) which are not detectable with conventional analytical protocols but are still possible to remobilize with changes of surrounding conditions, and thus will be bioavailable again. Therefore, there is a need to investigate thoroughly the long-term fate of NER-PFAS.

A multilayered scaffold for regeneration of smooth muscle and connective tissue layers.

Tissue regeneration often requires recruitment of different cell types and rebuilding of two or more tissue layers to restore function. Here, we describe the creation of a novel multilayered scaffold with distinct fiber organizations-aligned to unaligned and dense to porous-to template common architectures found in adjacent tissue layers. Electrospun scaffolds were fabricated using a biodegradable, tyrosine-derived terpolymer, yielding densely-packed, aligned fibers that transition into randomly-oriented fibers of increasing diameter and porosity.

Optimized microplastic analysis based on size fractionation, density separation and μ-FTIR.

Microplastic particles have been recognized as global hazardous pollutants in the last few decades pointing to the importance of analyzing and monitoring microplastics, especially in soils and sediments. This study focused on a multi-step approach for microplastic analysis combining grain size fractionation, density separation and identification by μ-FTIR-spectroscopy. Eight widely used polymers (polyethylene (PE), polypropylene (PP), polyvinylchloride (PVC), polystyrol (PS), polyethylenterephthalate (PET), polymethylmethacrylate (PMMA), polyurethane (PU) and polyamide (PA)) were fractionated into four groups of grain sizes (0.