Oxygen minimum zone cryptic sulfur cycling sustained by offshore transport of key sulfur oxidizing bacteria.

Members of the gammaproteobacterial clade SUP05 couple water column sulfide oxidation to nitrate reduction in sulfidic oxygen minimum zones (OMZs). Their abundance in offshore OMZ waters devoid of detectable sulfide has led to the suggestion that local sulfate reduction fuels SUP05-mediated sulfide oxidation in a so-called "cryptic sulfur cycle". We examined the distribution and metabolic capacity of SUP05 in Peru Upwelling waters, using a combination of oceanographic, molecular, biogeochemical and single-cell techniques.

Metabolic versatility of a novel N -fixing Alphaproteobacterium isolated from a marine oxygen minimum zone.

The N -fixing (diazotrophic) community in marine ecosystems is dominated by non-cyanobacterial microorganisms. Yet, very little is known about their identity, function and ecological relevance due to a lack of cultured representatives. Here we report a novel heterotrophic diazotroph isolated from the oxygen minimum zone (OMZ) off Peru.

Aerobic Microbial Respiration In Oceanic Oxygen Minimum Zones.

Oxygen minimum zones are major sites of fixed nitrogen loss in the ocean. Recent studies have highlighted the importance of anaerobic ammonium oxidation, anammox, in pelagic nitrogen removal. Sources of ammonium for the anammox reaction, however, remain controversial, as heterotrophic denitrification and alternative anaerobic pathways of organic matter remineralization cannot account for the ammonium requirements of reported anammox rates.

Facets of diazotrophy in the oxygen minimum zone waters off Peru.

Nitrogen fixation, the biological reduction of dinitrogen gas (N2) to ammonium (NH4(+)), is quantitatively the most important external source of new nitrogen (N) to the open ocean. Classically, the ecological niche of oceanic N2 fixers (diazotrophs) is ascribed to tropical oligotrophic surface waters, often depleted in fixed N, with a diazotrophic community dominated by cyanobacteria. Although this applies for large areas of the ocean, biogeochemical models and phylogenetic studies suggest that the oceanic diazotrophic niche may be much broader than previously considered, resulting in major implications for the global N-budget.

Giant hydrogen sulfide plume in the oxygen minimum zone off Peru supports chemolithoautotrophy.

In Eastern Boundary Upwelling Systems nutrient-rich waters are transported to the ocean surface, fuelling high photoautotrophic primary production. Subsequent heterotrophic decomposition of the produced biomass increases the oxygen-depletion at intermediate water depths, which can result in the formation of oxygen minimum zones (OMZ). OMZs can sporadically accumulate hydrogen sulfide (H2S), which is toxic to most multicellular organisms and has been implicated in massive fish kills.

Fragment recruitment on metabolic pathways: comparative metabolic profiling of metagenomes and metatranscriptomes.

The sheer scale of the metagenomic and metatranscriptomic datasets that are now available warrants the development of automated protocols for organizing, annotating and comparing the samples in terms of their metabolic profiles. We describe a user-friendly java program FROMP (Fragment Recruitment on Metabolic Pathways) for mapping and visualizing enzyme annotations onto the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways or custom-made pathways and comparing the samples in terms of their Pathway Completeness Scores, their relative Activity Scores or enzyme enrichment odds ratios. This program along with our fully configurable PERL-based annotation organization pipeline Meta2Pro (METAbolic PROfiling of META-omic data) offers a quick and accurate standalone solution for metabolic profiling of environmental samples or cultures from different treatments.

Doubling of marine dinitrogen-fixation rates based on direct measurements.

Biological dinitrogen fixation provides the largest input of nitrogen to the oceans, therefore exerting important control on the ocean's nitrogen inventory and primary productivity. Nitrogen-isotope data from ocean sediments suggest that the marine-nitrogen inventory has been balanced for the past 3,000 years (ref. 4).

Adherent-cell-dependent stimulation of CFU-GM by nucleobases, nucleosides, their analogues, and the hemoregulatory peptide dimer.

At suboptimal concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF), nucleobases and nucleosides as well as their analogues strongly stimulated aggregate (colony and cluster) formation from murine bone marrow granulocyte-macrophage colony-forming units (CFU-GM) in agar culture. Active compounds include 2'-deoxycytidine, thymidine, 5'-deoxyarabinosyl-cytosine, 5'-deoxy-5'-fluorothymidine, uracil, 6-methyluracil, orotic acid, and also purine derivatives as adenine, guanine, adenosine, and guanosine. The stimulation was almost identical to that obtained with the dimer of the hemoregulatory pentapeptide.

Cisplatin induces the small heat shock protein hsp25 and thermotolerance in Ehrlich ascites tumor cells.

Exposure of Ehrlich ascites tumor (EAT) cells to the anticancer drug cisplatin results in an elevated abundance of three isoforms of the small heat shock protein hsp25 without inducing the general stress response as commonly observed after heat shock. The most effective cisplatin concentration (2.5 microM) is also most efficient in arresting cells in S phase suggesting a relationship between hsp25 expression and cell cycle events.

Urinary retention in the course of neuroleptic therapy with haloperidol.

A case history was presented of a 49-year-old female patient, who had developed paranoid-hallucinatory schizophrenia for the first time and suffered from an acute functional bladder obstruction while receiving haloperidol. Thorough urological examination showed no pathologic findings except for a medium-grade urinary tract infection. No beneficial effects were obtained after application of parasympathicomimetic substances (carbachol, distigminebromide).

The colony-to-cluster ratio in agar cultures of bone marrow. II. Influence of a GM-CFC inhibiting factor.

An inhibitory activity for the proliferation of granulocyte-macrophage colony-forming cells (GM-CFC) of mouse bone marrow in soft-agar cultures was partly purified from rat bone marrow conditioned medium by ultrafiltration and gel chromatography. It was selective in that it also inhibited the proliferation of myeloid but not that of lymphatic mouse leukaemia cells. Even at strong (greater than 60%) inhibition of the formation of cell clones (colonies + clusters) the colony-to-cluster ratio (CClR) remained as in the controls.

The colony-to-cluster ratio in agar cultures of bone marrow. I. Constancy under standardized conditions and alteration in tumour-bearing mice and by various inhibitors added in vitro.

The growth of granulocyte-macrophage colony forming cells (GM-CFC) from mouse bone marrow was studied in agar cultures in glass capillaries. Under standard conditions with normal bone marrow a specific total number of cell clones (colonies + clusters) and also a specific colony-to-cluster ratio (CClR) was found for each of 4 different mouse strains. Both parameters were studied in tumour-bearing mice and found to be influenced independently and, in part, in an opposite way.

Aspects of chalone action.

The following aspects of action of a chalone-like factor for the Ehrlich ascites mammary carcinoma and of the granulocytic chalone are discussed: 1) Dependence on the state of proliferation. Rapidly growing cells respond poorly or not at all. 2) Antagonism with growth factors.

[Granulocyte chalone: tissue specificity of action in short-term cultures].

Granulocytic chalone containing extracts were obtained by incubating rat bone marrow cells in Hanks salt solution and further purification of the conditioned medium by ultrafiltration and gel chromatography. These extracts cause specific inhibition of 3H-thymidine incorporation in short-term cultures of rat bone marrow and murine myeloic leukemias. Ehrlich ascites tumour, spleen (mouse), lymphatic leukemia L1210 and melanoma AMel 3 (hamster) are not influenced under identical experimental conditions.