Use of immobilized antibodies in investigating acid alpha-glucosidase in urine in relation to Pompe's disease.

(1) A simple method is described for the isolation of the lysosomal enzyme, acid alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.

Properties of immobilized fig alpha-galactosidase and effect on ceramide-3 content of plasma from patients with Fabry's disease.

The possibility of lowering the level of ceramide-3 (galactosyl-alpha(1 leads to 4)-galactosyl-beta(1 leads to 4)-glucosyl-beta(1 leads to 1)-ceramide) in the plasma of patients with Fabry's disease was investigated. An immobilized alpha-galactosidase (alpha-D-galactoside galactohydrolase, EC 3.2.

A new immunochemical method for the quantitative measurement of specific gene products in man-rodent somatic cell hybrids.

An immunochemical method has been developed for the quantitative determination of species-specific gene products, for instance alpha-galactosidase and N-acetyl-alpha-galactosaminidase, in man-rodent hybrid cells and in the parental cell lines. Antisera raised against the purified enzymes are covalently coupled to Sepharose 4B. The gene products are specifically removed from a cell lysate by incubating with the appropriate Sepharose-coupled antiserum.

Enzymological properties and immunological characterization of alpha-galactosidase isoenzymes from normal and Fabry human liver.

1. A method is described for the rapid isolation of alpha-galactosidases A and B (alpha-D-galactoside galactohydrolase, EC 3.2.

Purification of chicken anti-bovine serum albumin antibody.

Chicken anti-bovine serum albumin antibody was isolated by precipitation with albumin at pH 8.6 in 13 per cent NaCl, solution of the precipitate at pH 11.3–11.

Heterogeneity of the antigenic centres of albumin toward chicken antibodies.

Pooled chicken antiserum to bovine serum albumin contained antibodies capable of binding eighteen sites on the albumin molecule. Quantitative study of the reactivity of albumin following gradual coupling to tyrosyl, histidyl and lysyl residues, indicated that these residues are in close relationship to the antigenic centres, and that the centres and their respective antibodies are heterogeneous.