Activation and deactivation of F0F1-ATPase in yeast mitochondria.

The regulation of membrane-bound proton F0F1 ATPase by the protonmotive force and nucleotides was studied in yeast mitochondria. Activation occurred in whole mitochondria and the ATPase activity was measured just after disrupting the membranes with Triton X-100. Deactivation occurred either in whole mitochondria uncoupled with FCCP, or in disrupted membranes.