Intrathecal ACNU treatment of B16 melanoma leptomeningeal metastasis in a new athymic rat model.

To evaluate new cytotoxic drugs for intrathecal treatment we developed an experimental model of leptomeningeal metastasis by intracisternal injection of 10(4) B16-F10 melanoma cells in nude rats. One hour in vitro incubation with 20 micrograms/ml ACNU (area under the drug concentration-time curve = 1200 microgramsxmin/ml) induced a 4-log kill of B16 melanoma cells. A single or repeated non-toxic dose of 1 mg/kg was injected into the cisterna magna of rats inoculated with tumor (area under the drug concentration-time curve assuming an even cerebrospinal fluid distribution greater than 7000 microgramsxmin/ml).

Radiotherapy in mice with yttrium-90-labeled anti-Ly1 monoclonal antibody: therapy of established graft-versus-host disease induced across the major histocompatibility barrier.

A monoclonal antibody recognizing Ly1, the murine homologue of CD5, was labeled with 90Y. In vivo biodistribution studies showed that 90Y-anti-Ly1 selectively localized in lymphoid tissue. Groups of B10,BR mice (H-2k) were lethally irradiated and given major histocompatibility complex-disparate C57BL/6 (H-2b) bone marrow and spleen cells to induce graft-versus-host disease (GVHD).

Radiotherapy in mice with yttrium-90-labeled anti-Ly1 monoclonal antibody: therapy of the T cell lymphoma EL4.

Yttrium-90 is a potent beta-emitting radionuclide with potential for therapy of lymphoma. A monoclonal antibody against Ly1, the murine homologue of human CD5, was labeled with 90Y and found to selectively bind to Ly1-positive, radiation-sensitive, EL4 mouse lymphoma cells. When tested in this aggressive model of T cell lymphoma, in vivo studies in C57BL/6 mice showed that a single 140-microCi i.

Antitumor activity of L6-ricin immunotoxin against the H2981-T3 lung adenocarcinoma cell line in vitro and in vivo.

The monoclonal antibody L6 recognizes a determinant that is expressed on lung, breast, colon, and ovarian carcinomas and is present only at trace levels in normal tissues. L6 was covalently linked to intact ricin by a thioether bond to produce an immunotoxin (IT). Gel analysis revealed that this IT was heterogeneous, but mostly one monoclonal antibody molecule linked to one ricin molecule.

Effects of human thyroxine-binding globulin and prealbumin on the reverse flow of thyroid hormones from extravascular space into the bloodstream in rabbits.

A plasma fraction rich in thyroid hormone-binding globulin (hTBG, human thyropexin) was injected iv into rabbits in order to see whether thyroid hormone concentrations in plasma would increase by return of T3 and T4 from the extravascular space. For this purpose, both [125I]T3 and [131I]T4 were simultaneously injected. After 1 h, or after 16 h in another series of experiments, 50 mg hTBG were injected iv.

[Misinterpreted expansive process of the middle lobe of the lung caused by unnoticed aspiration of a tooth].

In a patient of 78 years of age a diagnosis of a suspected expansive process of the middle lobe was made on the basis of the clinical findings and a plain radiography of the thorax. Fibre bronchoscopy revealed a subtotal stenosis of the middle lobe bronchus on the right side. The tissue sample revealed a chronically inflamed granulated tissue.

[Ophthalmologic and roentgenologic findings in a rare case of mucopolysaccharidosis 4 B].

A rare case of mucopolysaccharidosis, i.e., Morquio's disease with spondyloepiphyseal dysplasia, corneal clouding, normal intelligence, and deficiency of beta-galactosidase activity is described.

[Computed tomographic and clinical follow-up studies in intraventricular hemorrhage].

The investigation comprises 41 children with intraventricular hemorrhage (IVH). The CT-findings were divided into 4 groups, according to Papile. A CT and clinical follow-up study was performed on the survivors.

Morquio syndrome (mucopolysaccharidosis IV B) associated with beta-galactosidase deficiency. Report of two cases.

Two male patients, aged 6 and 25, both with normal intelligence and absence of neurological abnormalities, exhibited dysostosis multiplex, dwarfism, odontoid anomalies, cloudy corneas, exessive excretion of keratan sulfate, and abnormal urinary oligosaccharides. Leukocytes and fibroblasts of both patients were deficient in acid beta-galactosidase (beta-gal) and normal in N-acetylgalactosamine-6-sulfate sulfatase, the deficient enzyme in classical Morquio syndrome. The beta-gal deficiency was not due to an endogenous inhibitor, and the parents exhibited intermediate activities.

[Enzephalo-trigeminal angiomatosis. Sturge-Weber-Krabbe (author's transl)].

Following a case presentation clinical and radiological signs of the Sturge-Weber-Krabbe syndrome as well as its differentiation into three clinical entities according to Poser and Taveras are discussed. Calcification within the atrophic cerebral cortex is a predominant sign. Angiomatous lesions of the external ascending and descending cerebral veins, and occasionally of the internal cerebral veins may be demonstrated by angiography.

[Optimized cross-section determination for treatment planning in radiation therapy (author's transl)].

A true scale cross-section of the body is an indispensable condition for the accuracy of treatment planning in radiation therapy. To this end, ultrasonic or computed tomography may be used. Advantages and disadvantages of these methods are discussed.

[Recurrent purulent meningitis in traumatic lesions of the ethmoid (author's transl)].

A report is presented of two cases of recurrent meningitis (mainly pneumococcal meningitis with different serotypes). The different possible causes of recurrent meningitis are discussed. The key to diagnosis in recurrent meningitis caused by forgotten traumatic fracture of the ethmoid bone is X-ray investigation including tomography of this region.

[A complex malformation of the first and second branchial arch and of the first bran-hial arch and of the first branchial cleft. Dysostosis temporo-spheno-facialis (author's transl)].

A complex malformation of the first and second branchial arches and of the first branchial cleft is described; there was also involvement of the axial skeleton and the limbs. The syndrome has been described by us as dysostosis temporo-sphenofacialis and could not be classified with any previously described entity. The reason for this was due to the imcomplete description of previously published cases, but also the large number of possible combinations of changes in various organs which can occur.

[Renal osteopathy and bone mineralisation in patients treated with dihydrotachysterol during long term dialysis (author's transl)].

21 hypocalcemic patients on regular hemodialysis were treated for 2 months with 0.2 mg and for a further 2 months with 0.46 mg dihydrotachysterol daily.

[Lipoid dermatoarthritis (multicentric reticulohistiocytosis) (author's transl)].

The case of a 10-year-old girl with an infrequently observed lipid dermatoarthritis is reported. This is a systemic disease of unknown etiology characterized by a nodular eruption of the skin, mucosa and synovia, resulting in destructive arthritis and disfigurement of the facies. Biopsy of a nodule, whether from skin or synovia, discloses a cellular infiltrate consisting of histiocytes and multinucleated giant cells with lipid inclusions.