[Perinatal Presentation and Complicated Course of a Multifocal Atrial Tachycardia].

We report a male newborn who became symptomatic with supraventricular tachycardia on the first day of life. Neither adenosine nor electric cardioversion could terminate the tachycardia, therefore intravenous esmolol (β-receptor blocker) was initiated. Inspite of subsequent administration of various antiarrhythmic medications in increasingly higher doses, repeated supraventricular tachycardic episodes occurred.

Single step purification of recombinant proteins using the metal ion-inducible autocleavage (MIIA) domain as linker for tag removal.

For fast and easy purification, proteins are typically fused with an affinity tag, which often needs to be removed after purification. Here, we present a method for the removal of the affinity tag from the target protein in a single step protocol. The protein VIC_001052 of the coral pathogen Vibrio coralliilyticus ATCC BAA-450 contains a metal ion-inducible autocatalytic cleavage (MIIA) domain.

The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity - a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450.

Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of unknown function DUF1521.

Characterization of the self-cleaving effector protein NopE1 of Bradyrhizobium japonicum.

NopE1 is a type III-secreted protein of the symbiont Bradyrhizobium japonicum which is expressed in nodules. In vitro it exhibits self-cleavage in a duplicated domain of unknown function (DUF1521) but only in the presence of calcium. Here we show that either domain is self-sufficient for cleavage.

The evaluation of fluorescence changes after application of casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP) on early carious lesions.

Purpose: To evaluate the effect of casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP), as an adjunct to average oral hygiene, on the fluorescence of initial carious fissures and pits in vivo.

Methods: For this investigator-blind in vivo study 32 volunteers were recruited. Subjects with premolars and molars showing DIAGNOdent-values between 15 and 20 were randomly assigned to two treatment groups.

Four-year evaluation of a resin composite including nanofillers in posterior cavities.

Purpose: The aim of this prospective study was to evaluate the clinical performance of the restorative material Ceram.X in combination with an experimental one-bottle etch-and-rinse adhesive (K-0127).

Materials And Methods: A single operator placed two Class I or II restorations in molars of 43 patients.

Cleanliness of dentinal tubules following gutta-percha removal with and without solvents: a scanning electron microscopic study.

Aim: To determine the influence of solvents on gutta-percha and sealer remaining on root canal walls and in dentinal tubules.

Methodology: The root canals of 70 teeth were prepared chemomechanically to apical size 40. In group 1 (n = 10; control group), the canals remained unfilled.

Endodontic and salivary isolates of Enterococcus faecalis integrate into biofilm from human salivary bacteria cultivated in vitro.

Introduction: The aim of this study was to examine whether Enterococcus faecalis isolates from endodontic patients (from saliva and from a root canal) are able to prevail against salivary bacteria when grown in coculture in a biofilm reactor.

Methods: Saliva that was tested to be free of E. faecalis was used as the inoculum.

Combination of apex locator and endodontic motor for continuous length control during root canal treatment.

Aim: To compare ex vivo an experimental setup consisting of an electronic apex locator (EAL) and endodontic motor with an established product (Tri Auto ZX) for accuracy of length control during root canal treatment with three different types of files.

Methodology: An experimental setup consisting of porous spongy material and an electrolyte was used. Sixty anterior teeth were randomly assigned to six groups.

In situ fluoride retention and remineralization of incipient carious lesions after the application of different concentrations of fluoride.

Limited information is available on the time-dependent or dosage-dependent cariostatic efficacy of highly concentrated fluoride compounds. This good clinical practice-conforming, double-blind, placebo-controlled, crossover in situ study tested the hypothesis that a 1.0% amine fluoride fluid is superior to a 0.

New bacterial compositions in root-filled teeth with periradicular lesions.

The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing.

Survival of glass fiber post restorations over 5 years.

Purpose: To evaluate the survival of glass fiber reinforced composite post (GFP) restorations and to identify risk factors for restoration failure.

Methods: GFPs of three consecutively placed post systems, two tapered and one parallel-sided, were adhesively luted and the core was built with a resin composite. Teeth served as abutment teeth according to the prosthetic treatment plan.

Fluoride uptake and remineralisation of enamel lesions after weekly application of differently concentrated fluoride gels.

There is only little information on the remineralising capability of regularly applied highly concentrated fluoride gels. The aim of this in situ study was to test the hypothesis that weekly application of a 1.25% fluoride gel results in a significantly higher fluoride uptake and a significantly higher mineral gain compared to a 0.

"Rapid two-stage" Norwood operation in a child with multiorgan failure.

The Norwood I operation continues to be a procedure with significant operative mortality. One well-accepted risk factor for death after the first step of the Norwood operation is critical preoperative status. We describe herein a new concept for the treatment of patients with hypoplastic left heart syndrome (HLHS) in very poor preoperative condition.

Characterization of the first oral vagococcus isolate from a root-filled tooth with periradicular lesions.

Strain Endo-EH was isolated from a root-filled tooth associated with periradicular lesions. After subculturing on Columbia blood agar, phenotypic and genomic characterizations using different biochemical test systems, automated ribotyping, MALDI-TOF mass spectronomy, antibiotic susceptibility testing, and 16S rRNA gene sequence analysis were applied for further analysis. Phenotypic characterization identified this strain as Vagococcus fluvialis.

Pleural effusion: beta-trace protein in diagnosing ventriculoperitoneal shunt complications.

Catheter dysfunction is a common complication with ventriculoperitoneal shunts. Apart from infection, obstruction, and leakage, migration of the shunt tip may cause particular problems. Pleural effusion is easily classified as a shunt complication if a transdiaphragmatic migration of a shunt can be demonstrated.

Remineralization of artificial interproximal carious lesions using a fluoride mouthrinse.

Purpose: To evaluate remineralization and fluoride uptake of demineralized enamel specimens in artificial interproximal spaces using a 250 ppm fluoride mouthrinse (100 ppm amine fluoride and 150 ppm sodium fluoride) compared to a placebo-mouthrinse.

Methods: This was a two-way, double-blind, in situ study. 24 volunteers wore intraoral appliances in the lower jaw with mounted demineralized enamel specimens forming an artificial interproximal space.

Effect of dentifrice containing 5000 ppm fluoride on non-cavitated fissure carious lesions in vivo after 2 weeks.

Purpose: This randomized clinical trial compared the ability of two dentifrices, one containing 5000 ppm fluoride (Duraphat) and the other 1450 ppm fluoride (Caries Protection, control) to reverse non-cavitated fissure carious lesions.

Methods: 30 adults having occlusal lesions with DIAGNOdent values between 10 and 20 were randomly assigned to two groups of 15 participants each. DIAGNOdent measurements were performed after cleaning the teeth with cleaning brushes and after additional cleaning using an airflow device.

Effect of adhesive resin cements and post surface silanization on the bond strengths of adhesively inserted fiber posts.

This study evaluated the tensile bond strengths and the effect of silanization of fiber posts inserted with different adhesive systems. Sixty DT Light Posts (size 1) were used. Thirty posts were pretreated with silane.

Influence of adhesive systems on bond strength between fiber posts and composite resin cores in a pull-out test design.

The aim of this study was to evaluate the effects of post surface conditioning with adhesive systems on tensile bond strength between two composite resin core systems and FRP posts (ER DentinPost). Forty-eight posts were trimmed at the coronal part, and the upper part of 3 mm was covered with a standardized composite resin core build-up. Twenty-four posts were treated with the respective adhesive systems.

Two-year clinical evaluation of Cerec 3D ceramic inlays inserted by undergraduate dental students.

Objective: To evaluate the clinical results of final-year dental students' clinical performance after a practical computer-based course and training in the new Cerec 3 method.

Method And Materials: Cerec 3D restorations (Sirona) were made and inserted in patients by 50 dental students. The students were in their final semester in the Department of Operative Dentistry.

Bond strength between fibre posts and composite resin cores: effect of post surface silanization.

Aim: To evaluate the tensile bond strength between two different composite resin cores and (Clearfil Core, MultiCore Flow) and fibre posts (DT Light Post), with and without silanization of the post surface.

Methodology: Forty fibre posts were shortened to a length of 15 mm. Specimens were then produced by covering the upper 3 mm of the posts with standardized composite core build-ups.

Detection and eradication of microorganisms in root-filled teeth associated with periradicular lesions: an in vivo study.

This study determined the presence of microorganisms by culture and polymerase chain reaction in asymptomatic root-filled teeth with periradicular lesions. Furthermore, a disinfecting regimen using sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), chlorhexidine digluconate (CHX) irrigation, and calcium hydroxide (Ca(OH)(2)) dressing was assessed. After removal of the root-filling material, specimens of 20 cases undergoing retreatment were sampled.

Effects of various forms of calcium added to chewing gum on initial enamel carious lesions in situ.

The purpose of this randomized, cross-over in situ study was to determine the effects of 4 chewing gums on artificial caries-like subsurface lesions. Two chewing gums (1 with zinc citrate and 1 without) contained dicalcium phosphate (3.9%), calcium gluconate (1.

In vivo comparison of working length determination with two electronic apex locators.

Aim: To compare the accuracy of two electronic apex locators (EALs) in the same teeth in vivo.

Methodology: The working lengths in 20 teeth with a single canal were determined with two different EALs (Root ZX; J. Morita Corporation, Tokyo, Japan and Raypex 5 VDW, Munich, Germany) before extraction.