Growth factor and cytokine characterization of canine platelet lysate with variable leukocyte concentration, plasma content, and heat-sensitive proteins.

Background: Platelet lysate is an acellular platelet product containing factors released from secretory granules, including cytokines and growth factors. This study aimed to evaluate different centrifugation methods used to prepare canine platelet lysate with variable content of leukocytes, plasma, and heat-sensitive proteins.

Methods: Whole blood was collected from six dogs and two double-spin preparation methods were used to generate the platelet-rich plasma with reduced (PRP) and high (L-PRP) concentration of leukocytes.

Development and evaluation of a novel topically applied sildenafil citrate hydrogel and its influence on wound healing in dogs.

Objective: To develop a topical sildenafil hydrogel and evaluate its effect on wound healing in dogs.

Animals: 6 purpose-bred, sexually intact, adult Beagles.

Procedures: Hydrogels containing sildenafil citrate, N-methyl-2-pyrrolidone, propylene glycol, and poloxamer 407 were developed.

Laparoscopic gonadectomy in a dog with 78,XX/78,XY chimerism and underdeveloped reproductive organs.

Case Description: A 1-year-old externally sexually intact female Great Dane was referred for further evaluation of abnormal and underdeveloped internal reproductive organs.

Clinical Findings: Physical examination findings included a cranioventrally displaced vulva and a grade 2/6 left apical systolic heart murmur. No uterus or ovaries were identified during abdominal ultrasonography.

Evaluation of suture reinforcement for stapled intestinal anastomoses: 77 dogs (2008-2018).

Objective: To determine the influence of oversewing a transverse staple line in functional end-to-end stapled intestinal anastomoses (FEESA) in dogs.

Study Design: Retrospective observational study.

Sample Population: Seventy-seven client-owned dogs that underwent 78 FEESA reinforced (n = 30) or not reinforced (n = 48) with suture at the transverse staple line.

Os clitoris in dogs: 17 cases (2009-2017).

Medical records of 17 client-owned dogs diagnosed with os clitoris on physical examination or diagnostic imaging were reviewed to describe the clinical signs and surgical management of this condition. All dogs were phenotypically female. The most common presenting complaints included an enlarged clitoris ( = 10), urinary tract infection ( = 5), and licking of the vulva ( = 3).

Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells.

Background: Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter the MSC phenotype, rendering these cells immunogenic. As a result of bovine-derived exogenous proteins expressed on the cell surface, MSCs may be recognized by the host immune system as non-self and be rejected. Platelet lysate (PL) may obviate some of these concerns and shows promising results in human medicine as a possible alternative to FBS.

Platelet lysate obtained via plateletpheresis performed in standing and awake equine donors.

Background: Platelet preparations containing growth factors, attachment factors, and enzymes are appealing to enhance healing of injured tissues and as an alternative to xenogenic serum in cell culture media. Plateletpheresis is commonly used to collect platelets in human medicine but has not been validated in horses.

Study Design And Methods: Plateletpheresis to collect platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment.

Haemogregarine infections of three species of aquatic freshwater turtles from two sites in Costa Rica.

Twenty-five black river turtles (Rhinoclemmys funerea) and eight white-lipped mud turtles (Kinosternon leucostomum) from Selva Verde, Costa Rica were examined for haemoparasites. Leeches identified as Placobdella multilineata were detected on individuals from both species. All turtles sampled were positive for intraerythrocytic haemogregarines (Apicomplexa:Adeleorina) and the average parasitemia of black river turtles (0.