Bifidobacterium inopinatum sp. nov. and Bifidobacterium denticolens sp. nov., two new species isolated from human dental caries.

In a previous investigation of bifidobacteria isolated from human dental caries (V. Scardovi and F. Crociani, Int.

Characterization of the plasmid pVS809 from Bifidobacterium globosum.

A plasmid from a B. globosum strain was cut with 38 restriction enzymes and a physical map was constructed. Out of a total of 121 clones from curing experiments, plasmid was lost in 58% and 100% for acridine orange and ethidium bromide curing agent respectively.

Bifidobacterial cell wall proteins (BIFOP) in Bifidobacterium globosum.

Nearly 150 strains of Bifidobacterium globosum were isolated from faeces of calf, chicken, lamb, rabbit and rat, from sewage, from rumen content and from human infant faeces between 1962 and 1973 and scored by SDS-PAGE for the presence of cell-wall-related proteins, i.e. BIFOP (bifid outer proteins); their apparent molecular masses ranged from 94.

Phase variations in Bifidobacterium animalis.

Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice versa was determined. Differences in protein components and in penicillin-binding proteins (PBPs) of the cells from different colony types are shown.

Related structures in the plasmid profiles of Bifidobacterium asteroides, B. indicum and B. globosum.

Seventy strains of B. asteroides isolated from the honey bees Apis mellifera and A. cerana raised in 15 different countries, 73 strains of B.

Related structures in the plasmid profiles of Bifidobacterium longum.

The plasmid profiles of 123 strains of B. longum were examined with the Southern blot-hybridization technique to reveal the existence of related structures. B.

Plasmids in the genus Bifidobacterium.

A total of 1461 bacterial isolates, representing 24 different species of the genus Bifidobacterium, were examined for the presence of plasmid DNA. Approximately 20% of the isolates contained detectable plasmids, but only four species were presented: B. longum, the predominant bifid species in the human intestine; B.

Immunological relationships among transaldolases in the genus Bifidobacterium.

Antisera were prepared against electrophoretically homogeneous transaldolase (dihydroxyacetone transferase, E.C. 2.

Starch gel electrophoresis of fructose-6-phosphate phophoketolase in the genus Bifidobacterium.

Three groups of nomenspecies of the genus Bifidobacterium were distinguished by the different mobility of their fructose-6-phosphate phosphoketolase in starchgel electrophoresis; there is apparently a close relatedness between electrophoretic type of phosphoketolase and habitat.