Evaluation of three mitochondrial DNA markers for species identification, genetic diversity assessment, and phylogenetic positioning of five Hyalomma tick species from Tunisia.

Hyalomma ticks are important vectors of pathogens affecting human and animal health. This study aimed to assess the outputs of three molecular markers (16S, 12S rRNA, and COI) for accurate tick species molecular identification, genetic diversity assessment, and phylogenetic positioning of Hyalomma tick specimens from Tunisia. A total of 20 tickspecimens were collected from different hosts including cattle, camels, and turtles in nine Tunisian governorates.

Development and field evaluation of PCR assays based on minimum length cDNA fragments required for and tick species delineation.

Introduction: and ticks are important genera that can transmit diseases to both animals and humans, including Crimean-Congo hemorrhagic fever, tick-borne encephalitis, and several types of spotted fever. The accurate identification of tick species is essential for the effective control and prevention of tick-borne diseases. However, traditional identification methods based on morphology can be challenging and subjective, leading to errors.

Screening and Analysis of Tunisian Isolates Reveal the Diversity of Phylogeographic Marker and the Conservation of OmpA Protein Vaccine Candidate.

Bovine anaplasmosis caused by is a disease responsible for serious animal health problems and great economic losses all over the world. Thereby, the identification of isolates from various bioclimatic areas in each country, the phylogeographic analysis of these isolates based on the most informative markers, and the evaluation of the most promising candidate antigens are crucial steps in developing effective vaccines against a wide range of strains. In order to contribute to this challenge, a total of 791 bovine samples from various bioclimatic areas of Tunisia were tested for the occurrence of DNA through gene fragment amplification.

Zoonotic Species in Small Ruminant Ticks From Tunisia.

Tick-borne rickettsioses present a significant public health threat among emerging tick-borne diseases. In Tunisia, little is known about tick-borne pathogens. Therefore, the aim of this study was to investigate the presence of species in small ruminant ticks from Tunisia.