Structural analysis of the ribosome assembly factor Nep1, an N1-specific pseudouridine methyltransferase, reveals mechanistic insights.

Nucleolar essential protein 1 (Nep1; also known as ribosomal RNA small subunit methyltransferase Nep1) is a crucial factor in forming small ribosomal subunits in eukaryotes and archaea. Nep1 possesses an S-adenosyl-L-methionine (SAM)-dependent SpoU-TrmD (SPOUT) ribosomal RNA (rRNA) methyltransferase (MTase) fold and catalyzes pseudouridine (Ψ) methylation at specific sites of the small subunit (SSU) rRNA. Mutations in Nep1 proteins result in a severe developmental disorder in humans and reduced growth in yeast, suggesting its role in ribosome biogenesis.

How depression impacts on road traffic accidents among older adults and elderly Indians: Evidence from large scale nationally representative survey.

Introduction: With increased urbanization the prevalence of important public health problems like road traffic accidents (RTA) and depression are surging. This study was aimed to determine the association between RTA and depression among Indian population aged ≥45 years.

Methods: Using Longitudinal Aging Study in India (LASI) dataset (April 2017-December 2018), we have conducted this study among older adults (45-59 years) and elderly (≥60 years) Indians.

Distribution and association of road traffic accident with depression among Indian population aged 45 years and above: nested multilevel modelling analysis of nationally representative cross-sectional survey.

Introduction: The prevalence of important public health problems like road traffic accidents (RTA) and depression are surging. This study was aimed to estimate distribution and determine the association between RTA and depression among Indian population aged 45 years and above: overall and stratified into age group, gender and across states/union territories as aspirants, achievers, and front runners.

Methods: Using Longitudinal Aging Study in India (LASI) dataset (April 2017-December 2018), we have conducted this study among middle aged (45-59 years) and older adults and elderly (≥ 60 years) Indians.

Nested multilevel modelling study of smoking and smokeless tobacco consumption among middle aged and elderly Indian adults: distribution, determinants and socioeconomic disparities.

Introduction: The Global Adult Tobacco Survey (GATS) shows a drop in tobacco use worldwide. Despite the drop, there still continues to be a significant number of tobacco users in India. Research on tobacco use among young persons is commonly prioritised in India, while studies on tobacco use among middle-aged (45-59 years) and elderly (≥ 60 years) adults are noticeably lacking.

Unraveling the complexities of colorectal cancer and its promising therapies - An updated review.

Colorectal cancer (CRC) continues to be a global health concern, necessitating further research into its complex biology and innovative treatment approaches. The etiology, pathogenesis, diagnosis, and treatment of colorectal cancer are summarized in this thorough review along with recent developments. The multifactorial nature of colorectal cancer is examined, including genetic predispositions, environmental factors, and lifestyle decisions.

Decoding Substrate Selectivity of an Archaeal RlmCD-like Methyltransferase Through Its Salient Traits.

5-Methyluridine (mU) rRNA modifications frequently occur at U747 and U1939 ( numbering) in domains II and IV of the 23S rRNA in Gram-negative bacteria, with the help of -adenosyl-l-methionine (SAM)-dependent rRNA methyltransferases (MTases), RlmC and RlmD, respectively. In contrast, Gram-positive bacteria utilize a single SAM-dependent rRNA MTase, RlmCD, to modify both corresponding sites. Notably, certain archaea, specifically within the group, have been found to possess two genes encoding SAM-dependent archaeal (tRNA and rRNA) mU (ArmU) MTases.

Structural and functional characterization of archaeal DIMT1 unveils distinct protein dynamics essential for efficient catalysis.

Dimethyladenosine transferase 1 (DIMT1), an ortholog of bacterial KsgA is a conserved protein that assists in ribosome biogenesis by modifying two successive adenosine bases near the 3' end of small subunit (SSU) rRNA. Although KsgA/DIMT1 proteins have been characterized in bacteria and eukaryotes, they are yet unexplored in archaea. Also, their dynamics are not well understood.

Utilization of a trinuclear Cu-pyrazolate inorganic motif to build multifunctional MOFs.

The current work aims to generate multifunctional MOFs by incorporating a well-known inorganic motif, a trinuclear Cu-pyrazolate [Cu(μ-OH)(μ-Pyz)] (T-CuP) unit, as a node of the network. Accordingly, we report herein the synthesis and properties of five new compounds using five V-shaped dicarboxylic acids as auxiliary ligands. The structural features are consistent with the theme of grafting T-CuP units as nodal points of architectures whose chassis are primarily made of bent acids.

Exploring the Water Oxidation Catalytic Activity of a Mn-Based Magnetic Metal-Organic Framework: The Role of Proton Conductivity and Oxygen Evolution Reaction Overpotential.

The present work evaluates the water oxidation catalytic activity of a Mn-based metal-organic framework (MOF), which we envisioned to reduce the oxygen evolution reaction (OER) overpotential because of its high electrical conductivity, facilitated by solvent-encapsulated structural features. The presence of Mn centers induces interesting magnetic features in the MOF, which exhibits impressive cryogenic magnetic refrigeration with a Δ value of 29.94 J kg K for a field change of Δ = 5T at 2.

Distinct characteristics of putative archaeal 5-methylcytosine RNA methyltransferases unveil their substrate specificities and evolutionary ancestries.

5-Methylcytosine methyltransferases (mC MTases) are known to be involved in the modification of RNA. Although these enzymes have been relatively well characterized in bacteria and eukarya, a complete understanding of the archaeal counterparts is lacking. In this study, the identification and characterization of archaeal RNA mC MTases were performed.

A cross-sectional study exploring the positive aspects of caregiving in opioid dependence and its relation with quality of life, social support, and caregivers' burden.

Background And Aims: Despite a large body of research linking caregiver burden and social support in substance dependence, positive aspects of caregiving in these disorders have received very minimal attention. This exploratory observational study aimed to assess the positive aspects of caregiving for opioid dependence and evaluate the association of these positive aspects with caregiver quality of life, burden, and social support.

Methods: This cross-sectional study included 199 caregivers of patients with opioid dependence recruited through purposive sampling.

A tetracobalt(II) cluster with a two vertex truncated dicubane topology endogenously supported by carboxylate-based (2-pyridyl)methylamine ligands: magneto-structural and DFT studies.

A reaction between CoCl and L-(CO) (2 : 1 stoichiometry) in CHOH affords a discrete complex [CoII4-{L-(CO)}(μ-OCH)(CHOH)(HO)Cl] (1) [L-(CO) = 3-[-{2-(pyridin-2-yl)methyl}amino]-bis(propionate)]. The structure of 1 reveals two terminal mononuclear Co{L-(CO)}Cl units connected by a dimeric CoII2(μ-OCH)(CHOH)(HO) unit present in the centre through two methoxo (μ-OCH)- and two carboxylate (μ-1,1-OCO-) bridges affording a tetranuclear coordination cluster of Co(II) with a defective dicubane topology. In 1, Co1 (terminal) has distorted octahedral CoNOCl and the central Co2 has CoO coordination.

Biochemical Resistivity against Free Radicals and Microbes: Cooperative Action of Zn(II)/Imidazole in Phosphoesterase-Mediated Cell Death.

This work delivers a targeted synthesis of four isostructural O-substituted imidazole-based zinc(II) complexes, namely, , , , and , derived from homologous Schiff-base ligands and to explore their impact on free radicals, microbes, and dephosphorylation of phosphoesters. The antioxidant activity of all complexes was checked by various radical scavenging assays (ABTS, DPPH, and HO radical quenching). Among them, showed superior radical quenching activity, as indicated by its lowest EC50 value and thus maximum antioxidative capability.

Economic viability analysis of recycling waste plastic as aggregates in green sustainable concrete.

Plastic waste management is one of the major global challenges at present. Recycling single used plastic waste as partial replacement of natural aggregates in concrete may reduce problems regarding mismanagement of plastic waste and unsustainable utilisation of natural resources as aggregates. This concept has been explored in many studies and positive results are obtained, but it has not been materialized at a large scale due to the uncertainty regarding economic viability.

A Silver-Based Integrated System Showing Mutually Inclusive Super Protonic Conductivity and Photoswitching Behavior.

Photoinduced electricity and proton conductivity led fuel cells have emerged, , as highly promising systems for unconventional energy harvesting. Notwithstanding their individual presence with widely acclaimed results, an integrating system with mutually inclusive manifestation of both features has hitherto not been reported in the literature. To achieve this objective, our approach was to design a ligand system incorporating prerequisite features of both systems, like extended conjugation instigating photophysical activity and functional groups facilitating ionic conduction.

Pyridine-pyrazole based Al(iii) 'turn on' sensor for MCF7 cancer cell imaging and detection of picric acid.

We report herein the development of a new pyridine-pyrazole based bis-bidentate asymmetric chemosensor that shows excellent turn-on chelation-enhanced Al-responsive fluorescence. The presence of two 'hard' phenolic hydroxyl groups plays a pivotal role in switching-on the sensing through coordination to the 'hard' Al ion, while the mechanism can be interpreted by the chelation-enhanced fluorescence (CHEF) process. The X-ray single structure show a planar conjugated structure of the ligand, which was further stabilized by extensive H-bonding and π-π stacking.

Synergistic Experimental and Theoretical Studies of Luminescent-Magnetic LnZn Clusters.

The present work is part of our ongoing quest for developing functional inorganic complexes using unorthodox pyridyl-pyrazolyl-based ligands. Accordingly, we report herein the synthesis, characterization, and luminescence and magnetic properties of four 3d-4f mixed-metal complexes with a general core of LnZn (Ln = Dy, Gd, Tb, and Eu). In stark contrast to the popular wisdom of using a compartmental ligand with separate islands of and coordinating sites for selective coordination, we have vindicated our approach of using a ligand with overcrowded N-coordinating sites that show equal efficiency with both 4f and 3d metals toward multinuclear cage-cluster formation.

A Nd6 molecular butterfly: a unique all-in-one material for SMM, MCE and maiden photosensitized opto-electronic device fabrication.

Besides iron, ironically neodymium (Nd) is the most ubiquitously used metal for magnetic purposes, even among the lanthanides, when it comes to the field of molecular magnetism, yet it ranks among the least studied metals. However, strong apathy towards this magnetic lanthanide means that vital information will be missed, which is required for the advancement of the subject. Herein, we have successfully demonstrated the usefulness of a hexanuclear neodymium complex as a magnetic material, and also in electronic device fabrication.

Fascinating interlocked triacontanuclear giant nanocages.

We report herein three air, thermal and solvent stable interlocked triacontanuclear giant nanocages, generated using a node and spacer concept. Interestingly, the crystal structures of the cages are not only nano-dimensional but also exist in the nano-dimension range, which was corroborated with microscopic images. The combination of microscopic and crystallographic data, in effect, led us to a unique advantageous situation of generating nanomaterials with hard-to-come-by structural information at the molecular level.

Mobile application based early educational intervention for children with autism - a pilot trial.

Purpose: To design and develop a mobile application for pre-school readiness of children with autism and also to identify their learning pattern.

Methodology: Based on strong foundation of functional academics, we have developed a mobile application for providing an early educational intervention program to the children with autism. Three successive software trials have been conducted among 31 children with autism of age group of three to 10 years.

Lanthanide clusters of phenanthroline containing a pyridine-pyrazole based ligand: magnetism and cell imaging.

In this contribution, we report the synthesis, characterization and luminescence-magnetic properties of Ln-clusters (Ln = Gd3+, Eu3+ and Tb3+) using a new pyridine-pyrazole functionalized ligand fitted with a chromophoric phenanthroline backbone. The unorthodox N-rich ligand forms isostructural trinuclear lanthanide complexes with a topology that closely resembles two interdigitating hairpins. The clusters crystallize in chiral space groups and also exhibit chirality for bulk samples, which were further confirmed using solid state CD spectra.

Utilization of counter anions for charge transportation in the electrical device fabrication of Zn(II) metal-organic frameworks.

The present work reports the fabrication of anion-induced electrical devices with Zn(ii) metal-organic frameworks. The essence of our electronic device fabrication is to utilize the anionic species entrapped inside of the three-dimensional network of the MOFs for charge transportation. The idea is to generate MOFs as a host-guest system with encapsulated anions or anion-solvent clusters as guests and a cationic yet insulating three-dimensional framework as the host.

A free-standing, self-healing multi-stimuli responsive gel showing cryogenic magnetic cooling.

A novel Fe(iii)-based gel was synthesized via the self-assembly of Fe(iii) and pyridine 2,6 dicarboxylic acid. The synthesized gel has remarkable mechanical strength as well as self-sustainability. The metallogel also has thixotropic as well as self-healing properties.

Partially Solvated Dinuclear Ruthenium Compounds Bridged by Quinoxaline-Functionalized Ligands as Ru(II) Photocage Architectures for Low-Energy Light Absorption.

Ruthenium compounds with coordinated photolabile molecules that can be selectively released by irradiation with a visible light source are finding increasing applications in photoactivated chemotherapy (PCT) as photocages. Earlier photocages based on mononuclear Ru(II) compounds lack absorption in the therapeutic window (λ > 600 nm). In previous work, we synthesized the first partially solvated tppz bridged (tppz= 2,3,5,6-tetrakis(pyridin-2-yl)pyrazine) dinuclear Ru(II) complex capable of photoinduced ligand exchange at both metal centers.