Publications by authors named "Sayaka Miyazaki"
Article Synopsis
- Megalin is a multi-ligand endocytic receptor found in the apical membrane of proximal tubules and is noted for its presence in human urine in both ectodomain and full-length forms, with ectodomain being more prevalent.
- The study developed and validated two types of sandwich ELISAs—A-megalin and C-megalin—to specifically measure the ectodomain and full-length forms of megalin, respectively, in mouse urine.
- Findings indicated that A-megalin and C-megalin were detectable in normal mouse urine, but significantly reduced in mice with a megalin-knockout, highlighting the predominance of ectodomain forms in urine.
Aim: This study aimed to elucidate the 5-year changes in the cognitive function of elderly patients undergoing hemodialysis and its association with survival and related factors.
Methods: Cognitive function in patients aged ≥65 years undergoing hemodialysis at the Nagasaki Renal Center was assessed using the Mini-Mental State Examination (MMSE) in 2016. Patients were subsequently classified into normal, mild cognitive impairment (MCI) and suspected dementia groups according to their scores; MMSE was conducted at 30 and 60 months thereafter.
Article Synopsis
- The study aimed to find the best dose of sivopixant for treating refractory or unexplained chronic cough (RCC/UCC) through a phase 2b trial with various dosages compared to a placebo.
- Overall, while most patients completed the study, there were no statistically significant improvements in cough frequency or severity for any dose of sivopixant compared to placebo, although the 300 mg dose showed some promise.
- The trial indicated that sivopixant could lead to mild to moderate side effects, particularly taste disturbances, and emphasized the need for further research despite showing some potential benefits at the highest dosage.
Article Synopsis
- - The purinoceptor subtype P2X is important in the cough reflex and has been linked to taste disturbances; while gefapixant (an existing treatment) has low selectivity and causes taste issues, sivopixant is a new drug with higher selectivity for P2X receptors.
- - A clinical study involving 31 patients focused on the effects of sivopixant, administered for two weeks, assessing its efficacy in reducing cough and improving life quality, using a randomized, placebo-controlled crossover design.
- - Results showed that sivopixant significantly reduced cough frequency and improved health-related quality of life, with few mild side effects, including some taste disturbances in only 6.5% of participants. *
Aim: Although urinary incontinence (UI) in the elderly appears to be related to polypharmacy, it is unclear whether multiple medications elevate UI quantitatively or qualitatively. There have been few studies on the association of polypharmacy with each type of UI. The present survey aimed to clarify these issues.
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- - This study aimed to assess the effectiveness of a formulated food containing Peucedanum japonicum extract and saw palmetto extract in improving urination issues in men with lower urinary tract symptoms (LUTS) over a 4-week period.
- - Patients showed significant improvement in subjective symptom scores, especially in quality of life related to urination, nocturia frequency, and overall satisfaction; objective measures like residual urine volume also decreased significantly.
- - The treatment was well-received, with no adverse effects reported, and 75% of participants rated their experience positively, with many wanting to continue the treatment afterward.
Ganglioside GD3 is widely expressed in human malignant melanoma cell lines and tumors. Previously, we reported that GD3+ cells show stronger tyrosine phosphorylation of focal adhesion kinase (FAK), p130(Cas), and paxillin when treated with fetal calf serum than GD3- cells. In this study, we analyzed the changes in the signals mediated by the interaction between integrins and extracellular matrices (ECM) to clarify how GD3 enhances cell signals in the vicinity of the cell membrane.
View Article and Find Full Text PDFWe reported that ganglioside GD3 enhances cell proliferation and invasion of melanomas causing stronger tyrosine-phosphorylation of p130Cas and paxillin after stimulation with fetal calf serum. Besides signals via growth factor/receptor, adhesion signals via integrin might be also enhanced by GD3. Here, roles of integrin-mediated signaling in the cell proliferation and invasion, and in the activation of adaptor molecules were examined, showing that integrin was also important for the cell growth and invasion.
View Article and Find Full Text PDFMass spectrometry analysis of immunoprecipitates from serum-treated GD3-expressing melanoma cells with PY20 (anti-phosphotyrosine antibody) revealed that focal adhesion kinase (FAK) is more strongly activated in GD3-expressing cells than in GD3-negative cells. Involvement of FAK in the increased proliferation and invasion in GD3-expressing melanomas was demonstrated by siRNA-mediated knockdown. Also, it was shown that FAK is located up-stream of p130Cas and paxillin in the enhanced signaling pathway.
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