Identification of the epitope of 10-7G glycan antibody to recognize cancer-associated haptoglobin.

We previously identified fucosylated haptoglobin (Fuc-Hpt) as a clinical serum biomarker of pancreatic cancer and established the novel glycan monoclonal antibody (mAb) 10-7G. This antibody recognizes cancer-associated haptoglobin including Fuc-Hpt and the precursor of haptoglobin. Interestingly, Western blot analysis showed that the 10-7G mAb reacts with the haptoglobin α chain, which has no N-glycan potential sites; haptoglobin β chain has four N-glycan sites.

Establishment of an antibody specific for cancer-associated haptoglobin: a possible implication of clinical investigation.

We previously found that the serum level of fucosylated haptoglobin (Fuc-Hpt) was significantly increased in pancreatic cancer patients. To delineate the mechanism underlying this increase and develop a simple detection method, we set out to generate a monoclonal antibody (mAb) specific for Fuc-Hpt. After multiple screenings by enzyme-linked immunosorbent assay (ELISA), a 10-7G mAb was identified as being highly specific for Fuc-Hpt generated in a cell line as well as for Hpt derived from a pancreatic cancer patient.

[The influence of diet behavior and stress on binge-eating among female college students].

This study of 241 female college students examined the relationship among binge-eating, dieting, and stress, assessed at two different times. The results of path analysis showed significant paths both from binge-eating to dieting and from dieting to binge-eating were significant. In other words, binge-eating often results in dieting, and dieting often results in binge-eating, which becomes a vicious circle.