Evaluation of kinetic light scattering as an approach to the measurement of specific proteins with the centrifugal analyzer. II. Theoretical considerations.

Development of centrifugal analyzers capable of making light-scattering measurements has prompted the investigation of the application of this type of instrumentation to the measurement of specific proteins. Investigation of antigen/antibody complex dimension and the kinetics of the IgG/anti-IgG reaction as followed by light scattering are examined in relation to improved analytical methodology. The effect of nonspecific protein on the reaction rate has been investigated for IgM, and data are presented that provide insight into the mechanism of the effect of periodic mixing.

Evaluation of kinetic light scattering as an approach to the measurement of specific proteins with the centrifugal analyzer. I. Methodology.

A laser-modified centrifugal analyzer was used to develop kinetic light scattering methods for the measurement of human immunoglobulins IgG, IgA, and IgM. Comparison of equilibrium light scattering methods with kinetic procedures for IgG and IgA demonstrate equivalent precision and relative accuracy. However, quantitative results obtained by equilibrium and kinetics methods for IgM were found to differ significantly.

Serum and urine polyamines in cancer.

Polyamines in serum and urine have been found to be elevated in patients with cancer. A variety of methods employed for these measurements are discussed. Normal values obtained by the most recent methods are presented, and a survey has been made of polyamine levels in cancer patients.

A comprehensive gas chromatography procedure for measurement of drugs in biological materials.

Procedures have been developed for the determination in serum and urine of 21 drugs which include all of the barbiturates, methyprylon, glutethimide, meprobamate, propoxyphene, methaqualone, primidone, diphenylhydantoin, methadone, codeine, morphine, amphetamine and methamphetamine. The method involves pipeting 1.0 ml of serum or urine into an extraction tube, adding an internal standard together with either a pH 4.