ER stress-induced clearance of misfolded GPI-anchored proteins via the secretory pathway.

Proteins destined for the cell surface are first assessed in the endoplasmic reticulum (ER) for proper folding before release into the secretory pathway. This ensures that defective proteins are normally prevented from entering the extracellular environment, where they could be disruptive. Here, we report that, when ER folding capacity is saturated during stress, misfolded glycosylphosphatidylinositol-anchored proteins dissociate from resident ER chaperones, engage export receptors, and quantitatively leave the ER via vesicular transport to the Golgi.

Computational approach towards promoter sequence comparison via TF mapping using a new distance measure.

We propose a method for identifying transcription factor binding sites (TFBS) in the given promoter sequence and mapping the transcription factors (TFs). The proposed algorithm searches the +1 transcription start site (TSS) for eukaryotic and prokaryotic sequences individually. The algorithm was tested with sequences from both eukaryotes and prokaryotes for at least 9 experimentally verified and validated functional TFs in promoter sequences.

Computational approach towards finding evolutionary distance and gene order using promoter sequences of central metabolic pathway.

The comparative analysis of motifs of promoter sequences of the genes encoding enzymes of metabolic pathways such as glycolysis and kreb cycle in different genomes can give insights into the understanding of evolutionary and organizational relationships among both the species as well as enzymes. The comparison of resulting analysis with those of the evolutionary distances drawn considering coding regions of the genes allows one to measure the evolution of complete processes. In the present study we have collected promoter sequences of the glycolysis and kreb cycle genes encoding the respective enzymes from the standard EMBL database and extracted ten Transcription factors (TFs) using the TFsearch tool.