Expression of Genes Involved in Banana ( spp.) Response to Black Sigatoka.

This work aimed to evaluate the relative gene expression of the candidate genes , , , , and involved in the defense response to Black Sigatoka in banana cultivars Calcutta-4, Krasan Saichon, Grand Nain, and Akondro Mainty, by a quantitative real-time PCR. Biotic stress was imposed on 6-month-old plants during five sampling intervals under greenhouse conditions. The and genes were upregulated for the Calcutta-4- and Krasan Saichon-resistant cultivars, and were validated in this study.

On the feasibility of Vis-NIR spectroscopy and machine learning for real time SARS-CoV-2 detection.

The pandemic caused by Covid-19 is still present around the world. Despite advances in combating the disease, such as vaccine development, identifying infected individuals is still essential to optimize the control of human-to-human transmission of the virus. The main technique for detecting the virus is the RT-PCR method, which, despite its high relative cost, has a high accuracy in detecting the coronavirus.

Assessment of clinical characteristics and viral load in individuals infected by Delta and Omicron variants of SARS-CoV-2.

In late 2021, a new variant of SARS-CoV-2 called Omicron emerged, replacing Delta worldwide. Although it has been associated with a lower risk of hospitalization and severe forms of COVID-19, there is little evidence of its relationship with specific symptoms and viral load. The aim of this study was to verify the relationship between Delta and Omicron variants of concern, viral load, and the occurrence of symptoms in individuals with COVID-19.

Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study.

Background: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.

Methods: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.

Heart Disease and Arboviruses: A Systematic Review and Meta-Analysis.

Dengue fever, chikungunya, and zika are highly prevalent arboviruses transmitted by hematophagous arthropods, with a widely neglected impact in developing countries. These diseases cause acute illness in diverse populations, as well as potential cardiovascular complications. A systematic review was carried out to investigate the burden of cardiac involvement related to these arboviruses.