[Specificity of the interrelations of Leishmania and host cells in vitro].

Experiments on cross infection of peritoneal macrophages of mice with Leishmania of reptiles L. gymnodactyli and free cells of abdominal cavity of caucasian Agama (some part of which is composed by fibroblasts) with Leishmania of mammals L. major and L.

[Free amino acids in the blood serum of rats with liver failure developing as a consequence of the functioning of small intestine fistulae].

The experiments on 60 mongrel rats with hepatic failure due to small intestinal fistulas of high and medium location were carried out for the examination of amino acid spectrum in the animal plasma. 16 free amino acids were studied by ion-exchange chromatography. It has been established that hepatic failure due to small intestinal fistulas is characterized by the reduction of the total amino acid content by 20-30%.

[Differentiation of Leishmania in culture].

During their cultivation on diphasic medium Leishmania donovani, L. major and L. gymnodactyli pass through the regular differentiation within the promastigote stage of the life cycle that involves a gradual change of the four main morphological forms: 1) extremely basophilic, short, wide, actively dividing cells; 2) lightening longer forms with pointed posterior ends; 3) long, cigar-shaped uniformly light specimens with the clear nucleus in the central or posterior position and distinct karyosome; 4) small narrow nearly non-basophilic cells with a long compact nucleus and a rather large kinetoplast.

[Characteristics of the levels of various nucleic acid precursors in the tissues of rabbits with various thyroid conditions].

The content of the precursors and metabolites of nucleic acids in the brain and liver of normal rabbits, rabbits with hypothyrosis (thyroidectomy) and hyperthyrosis (100-150 Mg of L-thyroxine per 1 kg of body mass daily for 10-12 days) was determined by liquid chromatography with a high resolution. A decrease in the content of deoxyguanosine, thymine, hypoxanthine, cytosine, thymidine and an increase in the content of inosine, adenine and adenosine in the liver of animals with hypothyrosis were shown. In the brain of these rabbits the level of cytidine was lowered, and the levels of deoxyguanosine, thymine, inosine, hypoxanthine, adenine and uracil were on an increase.

[Genetics and evolution of the Lpm-system in American mink. VI. Identification, genetic control and phylogenesis of the new allotype Lpm13].

Data on immuno- and biochemical identification, genetic control and phylogenesis of new allotype Lpm13 of the Lpm system in domestic mink are presented. This allotype is encountered in mink populations with the frequency 0.9 and higher.

[Interrelations of a low-virulence strain of Toxoplasma gondii and inbred mice during late periods of observation].

Distinct differences in the intensity and dynamics of the infection with cysts of toxoplasms of law virulent (Czech) strain of brain of mice of three different pure lines and strainless ones differing in the sensitivity to the agent during a chronic period of infection have been noted. In sensitive mice of AKR line the period of formation and growth of cysts as well as the period of their elimination from the brain is longer than in less sensitive ones. In this connection cysts in the brain of these mice turned out to be larger than in the brain of less sensitive mice of CC57BR line.

[Comparative effectiveness of the beta-(N,N-diethylamino)ethylamide hydrochloride of fusaric acid, obzidan and atenolol in experimental adrenaline-induced cardiac arrhythmia].

Hydrochloride beta-(N,N-diethylamino)ethylamide of fusaric acid (DAEA) exerted an antiarrhythmic activity in adrenaline-induced arrhythmia in rats. DAEA single pretreatment in doses of 1-5 mg/kg prevented the disorders of rhythm and conductivity in most animals. A pronounced antiarrhythmic effect was manifest at doses of 2 and 4 mg/kg of DAEA.

[The rate of outflow of penetrating cations does not depend on the energy state of mitochondria].

Inhibiton of respiration at 0 degrees C believed to decrease the membrane potential in mitochondria did not lead to a substantial acceleration of the efflux of penetrating cations (triphenylmethylphosphonium, Cs- valinomycin). On the contrary the energy-dependent influx of the cations was rapidly inhibited indicating that there was no prolonged delay in the membrane deenergization. Low sensitivity of the cation efflux to respiration inhibition suggests under the conditions studied the metabolic component of the membrane potential to be rather small.

[Shift in the allele frequencies of the Ld locus in mink stock infected with Aleutian disease].

An idea on organization of the Ld-system of low density lipoprotein in the domestic mink as on a "closed" immunogenetic system with two codominant alleles Ld1 and Ld2 was confirmed. Significant differences in frequencies of genes and genotypes of the Ld-system between state farm "populations" unaffected with Aleutian disease and those which were centres of this epizootic were established. The results obtained confirm the assumption made earlier on subvitality of the Ld2 gene.

[Action of the salts of univalent thallium on the liver mitochondria of the rat].

Using light scattering and electron microscopic techniques, studies have been made of the swelling of liver mitochondria in isotonic sucrose solutions containing different concentrations of nitrate or acetate of monovalent thallium. It was concluded that the injurious effect of thallium on the mitochondria is associated with both its penetration into intramitochondrial space, and interaction of the external thallium with mitochondrial membranes.

[Morphofunctional preservation of rat liver mitochondria incubated at 0 degrees C].

Studies have been made on the valinomycin-induced transport of Cs+ at 0 degree C in rat liver mitochondria energized with succinate. Under these conditions, mitochondria preserve their ability to accumulate Cs+ for 6 hours of incubation or even longer. The rate of both the inward and outward movement of Cs+ at 0 degree C was approximately an order lower than at room temperature, although the stationary distribution of Cs+ remained at a high level.

Intramitochondrial reductive carboxylation of 2-oxoglutarate in adipose tissue and its contribution to fatty acid synthesis.

The reductive carboxylation of 2-oxoglutarate was found to proceed in mitochondria of rat epididymal fat pads and rabbit perirenal adipose tissue at a rate similar to that in liver mitochondria. In rat fat pads the incorporation of 14C from [5-14C]2-oxoglutarate into fatty acids via the carboxylation was suppressed by butylmalonate by 30%. 2-Oxoglutarate and glutamate stimulated the incorporation into fatty acids of 14C from [2-14C]acetate in rat fat pads with the simultaneous reduction of tissue NADP.

[Effect of hormones and partial hepatectomy on incorporation of labeled precursor in nuclear proteins of mouse liver].

Partial hepatectomy altered the nuclear protein synthesis, most distinct for H1 histones, within 1,5-3 hrs after the operation. Laparotomy, administration of hormones and dibutyryl-cAMP did not affect the cell nuclear apparatus throughout similar to partial hepatectomy. The cell response to partial hepatectomy appears to develop involving a number of factors between which unspecific stress reactions and hormones carry out a definite role.

Switch on and switch off phenomenon of liver gluconeogenic function in lamprey (Lampetra fluviatilis L.) under the influence of season and temperature.

The potential activity of pyruvate carboxylase in lamprey liver is the same as in mammals. However, at certain stages of the life cycle this reaction does not take place because of ATP deficiency in mitochondria. Energy charge potential of liver cells ranges from 0.

[Effect of a single dose of hydrocortisone and adrenaline on the synthesis of nuclear proteins and DNA in the rat thymus].

Single injections of hydrocortisone and adrenaline low doses long before the rat killing induced a change in the level of thymic DNA and nuclear protein synthesis. The effect and direction of hormone activity were dependent on the day time and duration of hormone presence in the organism. Hydrocortisone in a dose of 10 mg/kg, injected at 11 o'clock, promoted thymic DNA synthesis, whereas adrenaline in a dose of 0.

Uptake of thallous ions by mitochondria is stimulated by nonactin but not by respiration alone.

Nonrespiring rat-liver mitochondria swell in media containing high concentrations of thallous nitrate, indicating passive penetration of Tl+. This swelling could be further stimulated by 10 nM or more nonactin while even 1 microM valinomycin was without effect. Nonactin was also much more potent than valinomycin in stimulating swelling of respiring mitochondria in the presence of thallous acetate.

Immunogenetic study on the polymorphism of serum alpha 2-lipoproteins in mink. IV. Diallelism at the Ld locus of low-density lipoprotein.

Antibodies against a new allotype, Ld2, of mink low-density lipoprotein (LDL) were obtained by alloimmunization with a preparation of this lipoprotein. The two known allotypes of LDL, designated Ld1 and Ld2, are coded for by codominant alleles of the autosomal Ld locus. This locus is probably involved in the genetic control of the whole serum pool of LDL molecules.

[Mechanism of the persistence of a less virulent strain of Toxoplasma gondii in the body of the host].

The diameters of toxoplasms' cysts in native preparations of brain of bredless and inbred mice (AKR and CC57BR) infected with the toxoplasms of Czech low-virulent strain were measured. It is shown that the growth of cysts ceases by the 7th week from the infection and the ratio between cysts of different sizes does not change for 38 weeks and more. The curve of the ratio between cysts of different sizes, which in the period of infectious process stabilization is close to the even distribution curve, is apparently a result of primary parasitemia.

[Effect of a single administration of hydrocortisone within various periods of a day on the incorporation of the labeled precursor into nuclear proteins of intact rat liver].

A single administration of hydrocortisone within various periods of a day led to distinct and dissimilar alterations in distribution of 14C-lysine in erythrocytes, blood plasma and in liver tissue homogenates. The hydrocortisone injection also changed the synthesis of nuclear proteins, especially during the day time. The ability of cell-targets to accept the hormonal signal depended on the functional state of cell chromatin, which is responsible for realization of the signal effect.