[Prognostic value of bone marrow lesions in children with neuroblastoma detected by flow cytometry].

The purpose of the study was to evaluate the prognostic value of the detection of tumor cells in the bone marrow (BM) in children with neuroblastoma (NB) by flow cytometry. The detection of tumor cells was performed in BM of 51 patients with NB (24 boys and 27 girls) aged from 6 days to 15 years (median--1 year 3 months). Flow cytometry allowed determining NB cells in BM in a much larger number of cases than cytomorphology (49.

[Prognostic value of the determination of bone marrow lesion in patients with neuroblastoma based on the gene PHOX2B and TH expression].

Bone marrow (BM) involvement in neuroblastoma patients is commonly detected by cytomorphology and associated with poor outcome. Molecular techniques, flow cytometry and immunocytochemistry were offered to detect low number of tumor cells in BM due to high value of analytical sensitivity, while prognostic significance of results, obtained with these methods is unclear. PHOX2B and/or TH genes expression was selected as molecular marker of BM involvement.

[Methods of detection and prognostic significance of genetic material increase of the short arm of chromosome 2 and a deletion of the short arm of chromosome 1 in patients with neuroblastoma].

MYCN gene amplification and 1p deletion in neuroblastoma patients are associated with poor prognosis and commonly used for patient's stratification into risk groups. MYCN copy number and 1p deletion status were analyzed with multiplex ligase-dependent probe amplification (MLPA), PCR and FISH. MYCN amplification was revealed in 21 patients (17.

[The TH, ELAVL4 and GD2 gene expression as diagnostic markers of bone marrow lesions in patients with neuroblastoma].

The bone marrow (BM) TH, ELAVL4 and GD2 genes expression was evaluated in 331 samples from 57 different stage neuroblastoma (NB) patients, 26 BM samples from patients without NB and samples from 2 NB cell lines (IMR-32, Kelly) by real-time PCR. BM samples were considered NB-positive if PHOX2B expression was found or tumor cells were detected in BM smears. TH expression was not revealed in normal BM and was significantly lower in NB-negative samples.

[The limited possibility of using a simplified approach to detect minimal residual disease by the flow cytometry technique in children with precursor B-lineage acute lymphoblastic leukemia].

Minimal residue disease (MRD) is a state in which the tumor cells remain in the patient in the amounts unrecognizable with the standard cytological techniques. Flow cytometry is one of the basic methods for evaluation of MRD in precursor B-lineage acute lymphoblastic leukemia (PBLALL). The so-called simplified three-color analysis using the combination of CD19/CD10/CD34 antibodies has been proposed to detect MRD in the midcourse of induction therapy.

[Minimal residual disease monitoring by flow cytometry in children with acute lymphoblastic leukemia].

The cells that have avoided the action of antitumor drugs may be retained after remission achievement during induction therapy and consolidation. A combination of these cells is given the name minimal residual disease (MRD). Multicolor flow cytometry has recently attracted considerable interest as the most promising method for measuring the content of residual tumor blasts.

[Thyroid-stimulating hormone receptor antibodies in the diagnosis of Graves' disease in children].

The informative value of a method for determining total antibodies (Ab) to thyroid-stimulating hormone receptor (TSHR) in the diagnosis of Graves' disease was evaluated in 80 children with various causes of hyperthyroidism. With a RSHR Ab of > 1.6 IU/l, the diagnostic sensitivity and specificity of the test were 100%.

[The role of glycosaminoglycans in the local regulation of hemopoiesis in exposure of the body to extreme factors].

The authors studied the role of glycosaminoglycans as component of hematopoiesis-inducing microenvironment in the regulation of hematopoiesis. Following injection of preparations of these compounds to experimental animals (male CBA mice), their concentration changed most markedly in the bone marrow and spleen. The effect of acid glycosaminoglycans on the hematopoietic cells is realized through an increase of the concentration of calcium, cAMP, and leads to activation of granulocytopoiesis.

[Structure of mental diseases of the 2d half of life according to the results of an epidemiologic study].

An investigation of all patients (with the exception of alcoholics) residing in a city district and registered at a psychoneurological dispensary revealed that after 40 years the disease began in almost one-fourth of the cases (24.7%). In the majority of cases (56%) these were diseases characteristic of the second half of life: vascular pathology (45.

[Device for correcting elements of human walking by electric stimulation of muscles of the lower extremities].

In recent years electric stimulation of the lower limbs muscles in man while walking, particularly for cases of infantile cerebral paralysis, has found wide application. The device implementing correction of the pace components in walking must be carried directly by man. Therefore, it should be of a minature size, light weight, cheap and contain an autonomic power source.