Temporal genomic analysis of homogeneous tumor models reveals key regulators of immune evasion in melanoma.

Low intra-tumor heterogeneity (ITH) correlates with increased patient survival and immunotherapy response. However, even highly homogeneous tumors are variably aggressive, and the immunological factors impacting aggressiveness remain understudied. Here, we analyzed the mechanisms underlying immune escape in murine tumors with low ITH.

A comprehensive analysis of anthropometric indicators in preschool children suffering from recurrent respiratory infections.

Objective: Aim: To determine the state of relationship between anthropometric indicators and susceptibility to recurrent respiratory infections in preschool children.

Patients And Methods: Materials and Methods: A total of 143 children (73 boys and 70 girls) aged 12-59 months, undergoing inpatient treatment on acute respiratory infection, were involved in the clinical study. The number of acute respiratory infection episodes during a previous year of their lives was taken into account.

Surgical treatment and prognostic factors in perihilar tumors.

Objective: To study the results of surgical treatment in patients with perihilar tumors.

Material And Methods: We analyzed 98 patients with perihilar tumors who underwent surgery.

Results: We prefer percutaneous transhepatic biliary drainage (=58) for jaundice.

RNA Interference Effectors Selectively Silence the Pathogenic Variant c.607 G > A .

RNA interference (RNAi)-based therapeutics hold the potential for dominant genetic disorders, enabling sequence-specific inhibition of pathogenic gene products. We aimed to direct RNAi for the selective suppression of the heterozygous c.607 G > A variant causing encephalopathy.

Temporal genomic analysis of melanoma rejection identifies regulators of tumor immune evasion.

Decreased intra-tumor heterogeneity (ITH) correlates with increased patient survival and immunotherapy response. However, even highly homogenous tumors may display variability in their aggressiveness, and how immunologic-factors impinge on their aggressiveness remains understudied. Here we studied the mechanisms responsible for the immune-escape of murine tumors with low ITH.

Biomimetic Systems Involving Macrophages and Their Potential for Targeted Drug Delivery.

The concept of targeted drug delivery can be described in terms of the drug systems' ability to mimic the biological objects' property to localize to target cells or tissues. For example, drug delivery systems based on red blood cells or mimicking some of their useful features, such as long circulation in stealth mode, have been known for decades. On the contrary, therapeutic strategies based on macrophages have gained very limited attention until recently.

Fluorescent Probes with Förster Resonance Energy Transfer Function for Monitoring the Gelation and Formation of Nanoparticles Based on Chitosan Copolymers.

Nanogel-forming polymers such as chitosan and alginic acid have a number of practical applications in the fields of drug delivery, food technology and agrotechnology as biocompatible, biodegradable polymers. Unlike bulk macrogel formation, which is followed by visually or easily detectable changes and physical parameters, such as viscosity or turbidity, the formation of nanogels is not followed by such changes and is therefore very difficult to track. The counterflow extrusion method (or analogues) enables gel nanoparticle formation for certain polymers, including chitosan and its derivatives.

CRISPR-Cas9 correction in the DMD mouse model is accompanied by upregulation of Dp71f protein.

Duchenne muscular dystrophy (DMD) is a severe hereditary disease caused by a deficiency in the dystrophin protein. The most frequent types of disease-causing mutations in the DMD gene are frameshift deletions of one or more exons. Precision genome editing systems such as CRISPR-Cas9 have shown potential to restore open reading frames in numerous animal studies.

In-Frame Deletion of Dystrophin Exons 8-50 Results in DMD Phenotype.

Mutations that prevent the production of proteins in the gene cause Duchenne muscular dystrophy. Most frequently, these are deletions leading to reading-frame shift. The "reading-frame rule" states that deletions that preserve ORF result in a milder Becker muscular dystrophy.

CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the for safety studies of RNA therapeutics.

The development of personalized medicine for genetic diseases requires preclinical testing in the appropriate animal models. GNAO1 encephalopathy is a severe neurodevelopmental disorder caused by heterozygous mutations in the gene. c.

[Effective Viral Delivery of Genetic Constructs to Neuronal Culture for Modeling and Gene Therapy of GNAO1 Encephalopathy].

GNAO1 encephalopathy is an orphan genetic disease associated with early infantile epilepsy, impaired motor control, and severe developmental delay. The disorder is caused by mutations in the GNAO1 gene, leading to dysfunction of the encoded protein Gao1. There is no cure for this disease, and symptomatic therapy is ineffective.

Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

High expectations have been set on gene therapy with an AAV-delivered shortened version of dystrophin (µDys) for Duchenne muscular dystrophy (DMD), with several drug candidates currently undergoing clinical trials. Safety concerns with this therapeutic approach include the immune response to introduced dystrophin antigens observed in some DMD patients. Recent reports highlighted microutrophin (µUtrn) as a less immunogenic functional dystrophin substitute for gene therapy.

The Role Played by Transcription Factor E3 in Modulating Cardiac Hypertrophy.

Transcription factor E3 (TFE3), which is a key regulator of cellular adaptation, is expressed in most tissues, including the heart, and is reportedly overexpressed during cardiac hypertrophy. In this study, TFE3's role in cardiac hypertrophy was investigated. To understand TFE3's physiological importance in cardiac hypertrophy, pressure-overload cardiac hypertrophy was induced through transverse aortic constriction (TAC) in both wild-type (WT) and TFE3 knockout mice (TFE3).

Severe vancomycin-induced thrombocytopenia in a patient with meningitis.

Vancomycin is a widely used antibiotic and rarely can cause drug-induced thrombocytopenia. A patient with hospital-acquired meningitis after neurosurgery was treated with systemic and intrathecal vancomycin. On 9th day of antibiotic treatment, the patient's platelets dropped to 0.

ENZYMATIC ACTIVITY IN MICROSOMES, LIPID PEROXIDATION OF MICE HEPATOCYTES UNDER THE SODIUM FLUORIDE.

Fluorine is one of the most widespread and necessary microelements for the body of animals and humans, which is necessary in a clearly limited amount. Different concentrations of fluorine can affect the state of lipid peroxidation, as well as the functional state of the microsomes of liver hepatocytes. The studies were carried out on mature Wistar rats weighing 180-220 g.

Identification of Organic Matter Dispersions Based on Light Scattering Matrices Focusing on Soil Organic Matter Management.

The origin of organic matter, its spread, scattering, and functioning are influenced by the physical structure of liquid or dispersed media of organic matter. Refractive indices of fodder yeast grown on paraffin oil (paprin) and natural gas (gaprin) as well as Lycoperdon spore and organelles were measured by laser phase microscopy. The scattering matrices of aqueous suspensions of paprin, gaprin, and Lycoperdon spores were measured using a laser polarimeter with the scattering angle ranging from 20 to 150°.

The Clinico-Epidemiological Profile of Patients with Gonorrhoea and Challenges in the Management of Neisseria gonorrhoeae Infection in an STI clinic, Ternopil, Ukraine (2013-2018).

Gonorrhea is the second most common sexually transmitted infection spreading worldwide and a serious public health problem. However, further data are required to improve the management of gonorrhea. Our aim was to review the features of gonococcal infection and characterize the challenges of its management.

INVESTIGATION OF BONE MINERALIZATION IN PATIENTS WITH CORONARY HEART DISEASE COMPLICATED BY CHRONIC HEART FAILURE, STAGE II-A.

The majority of the studies have shown that individuals with cardiovascular diseases have a higher risk of experiencing bone loss and thus greater predisposition to risk of fracture. On the other hand there is growing evidence that individuals with low bone mass have higher mortality for cardiovascular events compared to patients with cardiovascular disease with normal bone mass. This research aims to investigate bone mineralization in patients with coronary heart disease complicated by stage II-A chronic heart failure.

Synthesis, Characterization, and Luminescent Properties of Polymer Complexes of Nd(III) with β-Dicarbonyl Ligands.

The neodymium(III) complexes with 2-methyl-5-phenylpenten-1-3,5-dione and allyl-3-oxo-butanoate were synthesized. The polycomplexes on their basis and copolymers with styrene and N-vinylcarbazole in ratio 5:95 were obtained by free-radical polymerization. The results of above studies have shown that the configuration of the chelate unit is unchanged during the polymerization.

In Situ Immobilization on the Silica Gel Surface and Adsorption Capacity of Poly[N-(4-carboxyphenyl)methacrylamide] on Toxic Metal Ions.

In situ immobilization of poly[N-(4-carboxyphenyl)methacrylamide] has been performed on silica gel surface. Infrared (IR) and mass spectroscopies as well as thermogravimetry (TG) analysis have been used to elucidate the structure of immobilized polymer. An adsorption capacity of the synthesized composite towards Cu(II), Pb(II), Mn(II), Fe(III), Co(II), and Ni(II) ions has been estimated.

Capture of heavy hydrogen isotopes in a metal-organic framework with active Cu(I) sites.

The production of pure deuterium and the removal of tritium from nuclear waste are the key challenges in separation of light isotopes. Presently, the technological methods are extremely energy- and cost-intensive. Here we report the capture of heavy hydrogen isotopes from hydrogen gas by selective adsorption at Cu(I) sites in a metal-organic framework.

[THE INFLUENCE OF NANODISPERSE CERIUM DIOXIDE ON ONTOGENETIC CHANGES OF ANTIOXIDANT SYSTEM IN THE MUCOSA OF THE STOMACH AND COLON IN RATS].

It was established that with age the content of lipid peroxidation products increased in the mucosa of the stomach: Diene conjugates by 30%, products which react to thiobarbituric acid by 285% and Schif bases by 181%. Nanodisperse cerium dioxide (NCD) reduced the content of lipid peroxidation in the gastric mucosa in old rats: Diene conjugates by 43 %, products which react to thiobarbituric acid by 51% and Schif bases by 44% relative to the control group of rats given age. Similarly, it was established that the content of Diene conjugates increased by 40%, products which react to thiobarbituric acid by 114% and Schif bases by 132% in the mucosa of the colon of old rats.

Electro- and photosensitive azopolymer for alignment of liquid crystals.

We report an electro- and photosensitive metal containing polymer material for alignment of liquid crystals (LCs). Irradiation with polarized light and/or application of dc-field result in an anisotropy of the polymer and formation of an easy orientation axes of a LC on the polymer surface. The light-induced anisotropy of the polymer and the LC anchoring on the polymer surface can be controlled by the low dc-field at room temperature.

[Role of single-nucleotide polymorphism C-1562т of the matrix metaloproteіnaza-9 gene in the development of leiomyoma in women with cervical pathology].

108 women diagnosed with leiomyoma and 84 women without this disease (control group) were examined during the research. Was conducted a comparative analysis of the genotypes distribution between women with cervical pathology of different complexity. The results of the research showed, that there is no connection between the С-1562Т polymorphism of the MMP-9 gene and the progression of leiomyoma the course of which is accompanied by false erosion of the cervix (ectopias epithelium) (p>0,521).