Tubulin Vibration Modes Are in the Subterahertz Range, and Their Electromagnetic Absorption Is Affected by Water.

Many proteins are thought to coordinate distant sites in their structures through a concerted action of global structural vibrations. However, the direct experimental spectroscopic detection of these vibration modes is rather elusive. We used normal-mode analysis to explore the dominant vibration modes of an all-atom model of the tubulin protein and described their characteristics using a large ensemble of tubulin structures.

On the rise of fear speech in online social media.

Recently, social media platforms are heavily moderated to prevent the spread of online hate speech, which is usually fertile in toxic words and is directed toward an individual or a community. Owing to such heavy moderation, newer and more subtle techniques are being deployed. One of the most striking among these is fear speech.

Conserved Dynamic Mechanism of Allosteric Response to L-arg in Divergent Bacterial Arginine Repressors.

Hexameric arginine repressor, ArgR, is the feedback regulator of bacterial L-arginine regulons, and sensor of L-arg that controls transcription of genes for its synthesis and catabolism. Although ArgR function, as well as its secondary, tertiary, and quaternary structures, is essentially the same in and , the two proteins differ significantly in sequence, including residues implicated in the response to L-arg. Molecular dynamics simulations are used here to evaluate the behavior of intact ArgR with and without L-arg, and are compared with prior MD results for a domain fragment of ArgR.

A new type low-cost, flexible and wearable tertiary nanocomposite sensor for room temperature hydrogen gas sensing.

This paper reports on reduced graphene oxide (rGO), tin oxide (SnO) and polyvinylidene fluoride (PVDF) tertiary nanocomposite thick film based flexible gas sensor. The nanocomposite of 0.90(PVDF) - 0.

Communication between N terminus and loop2 tunes Orai activation.

Ca release-activated Ca (CRAC) channels constitute the major Ca entry pathway into the cell. They are fully reconstituted via intermembrane coupling of the Ca-selective Orai channel and the Ca-sensing protein STIM1. In addition to the Orai C terminus, the main coupling site for STIM1, the Orai N terminus is indispensable for Orai channel gating.

Cholesterol modulates Orai1 channel function.

STIM1 (stromal interaction molecule 1) and Orai proteins are the essential components of Ca(2+) release-activated Ca(2+) (CRAC) channels. We focused on the role of cholesterol in the regulation of STIM1-mediated Orai1 currents. Chemically induced cholesterol depletion enhanced store-operated Ca(2+) entry (SOCE) and Orai1 currents.

Effect of Co-transfection of Anti-myostatin shRNA Constructs in Caprine Fetal Fibroblast Cells.

Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprine fetal fibroblast cells for transient silencing of MSTN gene. In the present investigation, approximately 89% MSTN silencing was achieved in transiently transfected caprine fetal fibroblast cells by cotransfection of two best out of four anti-MSTN shRNA constructs.

Binding-competent states for L-arginine in E. coli arginine repressor apoprotein.

Arginine repressor of E. coli is a multifunctional hexameric protein that provides feedback regulation of arginine metabolism upon activation by the negatively cooperative binding of L-arginine. Interpretation of this complex system requires an understanding of the protein's conformational landscape.

Assessment of developmental potential of caprine cloned embryos with ooplasm replenishment under two culture media.

The present study was designed to assess the developmental potential of somatic cell nuclear transfer (SCNT) embryos, with and without replenishment of ooplasm into enucleated oocytes, by culturing separately in two culture media. The enucleated oocytes were replenished with exogenous matured ooplasm under replenished nuclear transfer (RNT) method and compared with conventional nuclear transfer (CNT) method without replenishment. The fusion efficiency in RNT group was found to be significantly higher (P < 0.

Developmental potency of pre-implant parthenogenetic goat embryos: effect of activation protocols and culture media.

The developmental potency of pre-implant parthenogentic goat embryos were compared under two chemical activation protocols in three different culture media groups. The in vitro matured oocytes were chemically activated by two protocols viz. P1 (CB-CHX-6DMAP) and P2 (Ca-CHX-6DMAP).

Drug target prioritization in Plasmodium falciparum through metabolic network analysis, and inhibitor designing using virtual screening and docking approach.

The genome sequence of Plasmodium falciparum reveals that many metabolic pathways are unique as compared to its human host. Metabolic Network Analysis was carried out to find the essential enzymes critical for the survival of the pathogen. In the present study, choke point and load point analysis was used to locate putative targets.