Random cleavage of superhelical SV 40 DNA S1 nuclease.

SV40 DNA FO I is randomly cleaved by S1 nuclease both at moderate (50 mM) and higher salt concentrations (250 mM NaC1). Full length linear S1 cleavage products of SV40 DNA when digested with various restriction endonucleases revealed fragments that were electrophoretically indistinguishable from the products found after digestion of superhelical SV40 DNA FO I with the corresponding enzyme. Concordingly, when the linear S1 generated duplexes were melted and renatured, circular duplexes were formed in addition to complex larger structures.

[Subclavian vein catheterization in traumatic surgery (author's transl)A1].

The new Cavafix system was employed for puncture and catheterization of the subclavian vein in 100 casuality patients undergoing surgical intervention. Excellent results were achieved not only in emergency cases with severe shock and hypovolaemia, but also in hospitalized patients requiring long-term infusion therapy. This system proved easy to handle even by doctors inexperienced in the use of such techniques and, hence, the incidence of complications was within the range reported in the literature.

[Evaluation and wearing habits of complete dentures--results from a patient questionnaire].

In a questionnaire answered by 578 patients with new total dentures the personal attitude of the patient to his dental replacement was tested. Ninety per cent of the patients considered their dentures to be good or satisfactory, while 10 per cent were not satisfied. In addition, findings regarding the length of time for and manner in which dentures are being worn as well as denture care and expectations concerning prosthetical treatment are reported.

Size and distribution of SV 40 DNA Sequences covalently linked with the DNA of permissive mammalian cells.

CV-1 cells productively infected with SV 40 contain viral DNA which is covalently linked with the host cell DNA. These linear duplex viral-host DNA molecules are replicated during the infectious cycle. They can be selectively isolated and purified by two successive cycles of DNA-DNA hybridization and elution steps using first CV-1 cell and then SV 40-DNA immobilized on filters.

Fate of infecting simian virus 40-deoxyribonucleic acid in nonpermissive cells: integration into host deoxyribonucleic acid.

Nonpermissive 3T3 cells were infected with purified superhelical simian virus 40 (SV40) deoxyribonucleic acid I (DNA I). One hour after infection, approximately 60% of the intracellular SV40 DNA was converted to relaxed forms. One day after infection, all intracellular SV40 DNA was present as slow-sedimenting material, and no SV40 DNA I was detectable.