Dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of Cr6+ from real tannery effluent employing indigenous Bacillus cereus isolate.

A native Bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and Cr(6+) remediation from the real tannery effluent. Most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. The extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with B.

Process parameters for decolorization and biodegradation of orange II (Acid Orange 7) in dye-simulated minimal salt medium and subsequent textile effluent treatment by Bacillus cereus (MTCC 9777) RMLAU1.

In this study, Bacillus cereus isolate from tannery effluent was employed for orange II dye decolorization in simulated minimal salt broth and textile effluent. Most of the physicochemical parameters of textile effluent were above the permissible limits. The strain was highly tolerant to dye up to 500 mg l(-1).

Downstream processing, characterization, and structure-function relationship of solvent-, detergent-, psychro-, thermo-, alkalistable metalloprotease from metal-, solvent-tolerant psychrotrophic Pseudomonas putida SKG-1 isolate.

The purification and characterization of psychro-thermoalkalistable protease from psychrotrophic Pseudomonas putida isolate is being reported for the first time. A ~53 kDa protease was purified 21.4-folds with 57.

Pentachlorophenol dechlorination and simultaneous Cr6+ reduction by Pseudomonas putida SKG-1 MTCC (10510): characterization of PCP dechlorination products, bacterial structure, and functional groups.

It is the first report in which a novel psychrotrophic Pseudomonas putida SKG-1 strain was evaluated for simultaneous bioremediation of pentachlorophenol and Cr(6+) under various cultural and nutritional conditions. Pentachlorophenol (PCP) dechlorination products, bacterial structure, and functional groups were characterized by gas chromatography and mass spectrometry (GC-MS), scanning electron microscope and energy dispersive X-ray spectroscopy (SEM-EDS), and Fourier-transform infrared (FTIR) techniques. The strain was extremely tolerant to excessively higher individual concentration of PCP (1,400 mg l(-1)) and Cr(6+) (4,300 mg l(-1)).

Strategies for chromium bioremediation of tannery effluent.

Bioremediation offers the possibility of using living organisms (bacteria, fungi, algae,or plants), but primarily microorganisms, to degrade or remove environmental contaminants, and transform them into nontoxic or less-toxic forms. The major advantages of bioremediation over conventional physicochemical and biological treatment methods include low cost, good efficiency, minimization of chemicals, reduced quantity of secondary sludge, regeneration of cell biomass, and the possibility of recover-ing pollutant metals. Leather industries, which extensively employ chromium compounds in the tanning process, discharge spent-chromium-laden effluent into nearby water bodies.

Microbial dechlorination of chloroorganics and simultaneous decolorization of pulp-paper mill effluent by Pseudomonas putida MTCC 10510 augmentation.

The physicochemical analyses of pulp-paper mill effluent revealed that it was dark brown with 1761 ± 2.3 color PtCo units having slightly alkaline pH, high biological oxygen demand and chemical oxygen demand values, and contained large quantities of organic and inorganic constituents, well above the prescribed standards. The bacterial growth, color reduction, and dechlorination were evident in all the four sets of experiments with different possible combinations of nutrient supplementation and Pseudomonas putida augmentation.

Profiling of biodegradation and bacterial 16S rRNA genes in diverse contaminated ecosystems using 60-mer oligonucleotide microarray.

We have developed an oligonucleotide microarray for the detection of biodegradative genes and bacterial diversity and tested it in five contaminated ecosystems. The array has 60-mer oligonucleotide probes comprising 14,327 unique probes derived from 1,057 biodegradative genes and 880 probes representing 110 phylogenetic genes from diverse bacterial communities, and we named it as BiodegPhyloChip. The biodegradative genes are involved in the transformation of 133 chemical pollutants.

Quality assessment of treated tannery wastewater with special emphasis on pathogenic E. coli detection through serotyping.

The present study was undertaken to assess the quality of treated tannery effluent discharged from Common Effluent Treatment Plant (CETP), Unnao, India, to various water bodies and agricultural lands. The physico-chemical analyses revealed that the effluent was yellowish-brown in colour, having alkaline pH, high BOD, COD values and contained large quantities of organic and inorganic constituents well above the prescribed standards. The bacteriological quality was assessed in terms of the presence of heterotrophs and total as well as faecal coliforms.