Response of restraint stress-selected lines of Japanese quail to heat stress and Escherichia coli challenge.

Japanese quail selected for divergent corticosterone response to restraint stress were evaluated for their susceptibility to heat stress and challenge with Escherichia coli. These quail lines are designated as high stress (HS), low stress (LS), and the random-bred control (CS) lines. Heat stress (35°C, 8 h/d) began at 24 d until the end of the study at 39 d.

Fecal corticosterone metabolites and plasma corticosterone in Japanese quail selected for low or high plasma corticosterone responses to brief restraint.

Fecal corticosterone metabolites and plasma corticosterone in Japanese quail selected for low- or high-plasma corticosterone responses to brief mechanical restraint (low- and high-stress lines), and in a line of unselected quail, were measured in this study. No line differences were observed in baseline plasma corticosterone concentrations, but fecal corticosterone metabolite concentrations and daily fecal corticosterone metabolite production were 20% higher in quail of the high-stress line than in unselected or low-stress quail for males and females living together in group cages (P < 0.05).

Testosterone manipulation postcastration does not alter cloacal gland growth differences in male quail selected for divergent plasma corticosterone stress response.

Japanese quail selected for reduced (low-stress, LS) rather than exaggerated (high-stress, HS) plasma corticosterone response to brief restraint have consistently shown greater cloacal gland (CG) development, an androgen-dependent trait. In this study, the effects of testosterone implants on levels of plasma testosterone and CG development in castrated LS and HS quail were determined. Stress-line males were castrated and randomly allocated to 1 of 3 testosterone treatments: the empty testosterone (ET), low testosterone (LT), or high testosterone (HT) implant group.

Plasma corticosterone responses to handling in Japanese quail selected for low or high plasma corticosterone responses to brief restraint.

1. Plasma corticosterone responses to handling in Japanese quail selected for low or high corticosterone responses to brief mechanical restraint (low and high stress lines) were measured in this study. 2.

Short-term spatial memory responses in aged Japanese quail selected for divergent adrenocortical stress responsiveness.

Stress-induced glucocorticoids can dampen learning and spatial memory via neuronal damage to the hippocampus. Cognition losses can be transient (associated with acute stress episodes) or permanent as in aged individuals who show chronic glucocorticoid-induced accelerated brain aging and neurodegeneration (dementia). Thus, chronic versus acute stress effects on spatial memory responses of quail selected for reduced (low stress, LS) or exaggerated (high stress, HS) plasma corticosterone (B) response to brief restraint were assessed.

Neonatal maternal separation alters immune, endocrine, and behavioral responses to acute Theiler's virus infection in adult mice.

Previous studies have established a link between adverse early life events and subsequent disease vulnerability. The present study assessed the long-term effects of neonatal maternal separation on the response to Theiler's murine encephalomyelitis virus infection, a model of multiple sclerosis. Balb/cJ mouse pups were separated from their dam for 180-min/day (180-min MS), 15-min/day (15-min MS), or left undisturbed from postnatal days 2-14.

Influence of maternal corticosterone treatment on incubation length of eggs laid by Japanese quail hens selected for divergent adrenocortical stress responsiveness.

1. Previous studies have shown that more yolk corticosterone is found in the eggs of random bred Japanese quail hens implanted with corticosterone during egg formation; both unstressed and stressed quail hens selected for exaggerated (high stress) rather than reduced (low stress) plasma corticosterone response to brief restraint deposit more corticosterone into their egg yolks. The length of egg incubation is also known to be shorter in eggs laid by high than low stress hens.

Effect of the density of conspecifics on runway social reinstatement behavior of male Japanese quail genetically selected for contrasting adrenocortical responsiveness to stress.

Runway tests are considered indicative of underlying sociality in birds and their ability to make social discriminations and establish interactions among conspecifics. Herein, social reinstatement behavior in male juvenile Japanese quail selected for a reduced (LS, low stress) or exaggerated (HS, high stress) adrenocortical response to brief mechanical restraint was evaluated. Individual males were given the choice to reinstate with either 2 (low density, LD) or 8 (high density, HD) unfamiliar randombred conspecifics placed in goal boxes at opposite ends of a two-choice runway (TCRW).

The effects of testosterone on immune function in quail selected for divergent plasma corticosterone response.

The immunocompetence handicap hypothesis (ICHH) suggests that the male sex hormone testosterone has a dual effect; it controls the development and expression of male sexually selected signals, and it suppresses the immune system. Therefore only high quality males are able to fully express secondary sexual traits because only they can tolerate the immunosuppressive qualities of testosterone. A modified version of the ICHH suggests that testosterone causes immunosuppression indirectly by increasing the stress hormone corticosterone (CORT).

How acute is the acute stress response? Baseline corticosterone and corticosteroid-binding globulin levels change 24h after an acute stressor in Japanese quail.

Changes in plasma corticosteroid-binding globulin (CBG) capacity can alter free plasma concentration and tissue availability of glucocorticoids (GC) and hence alter the organismal response to stress. However, CBG change in response to stress has not been extensively studied. While it is clear that chronic stress can causes CBG decline and in some species acute stressors can reduce CBG during the 30-60 min of the stressor, more long-term changes in CBG following an acute stressor has received less attention.

Maternal corticosterone reduces egg fertility and hatchability and increases the numbers of early dead embryos in eggs laid by quail hens selected for exaggerated adrenocortical stress responsiveness.

Quail hens selected for exaggerated (HS, high stress) rather than reduced (LS, low stress) plasma corticosterone (B) response to brief restraint deposit more B into their egg yolks than do LS hens. Female progeny of HS hens implanted with B also show reduced egg production when compared with female offspring of LS- and HS-control and LS-B-implanted hens. Herein, LS and HS hens were implanted (s.

Length of incubation and hatchling body weight in quail selected for reduced or exaggerated adrenocortical responsiveness to brief restraint.

1. While changes in corticosterone (B) during late embryogenesis are thought to play a role in the hatching process, only a scant and controversial literature exists concerning the effects of in ovo B on the length of incubation and chick body weight at hatching. Because female Japanese quail selected for exaggerated (high stress, HS) rather than reduced (low stress, LS) plasma B response to brief mechanical restraint deposit more B into their eggs, these stress lines provide an excellent model to study the relationship between embryonic B and incubation length and hatchling body weight.

Fractal analysis of the ambulation pattern of Japanese quail.

1. The study examined the practicality and usefulness of fractal analyses in evaluating the temporal organisation of avian ambulatory behaviour by using female Japanese quail in their home boxes as the model system. To induce two locomotion activity levels, we tested half of the birds without disturbance (Unstimulated) and the other half when food was scattered on the floor of the home box after 3 h of feeder withdrawal (Stimulated).

Open-field temporal pattern of ambulation in Japanese quail genetically selected for contrasting adrenocortical responsiveness to brief manual restraint.

Japanese quail selected for a low-stress (LS), rather than a high-stress (HS), plasma corticosterone response to brief restraint have been shown to possess lower fearfulness and a nonspecific reduction in stress responsiveness. Detrended fluctuation analysis provides information on the organization and complexity of temporal patterns of behavior. The present study evaluated the temporal pattern of ambulation of LS and HS quail in an open field that represented a novel environment.

Influences of maternal corticosterone and selection for contrasting adrenocortical responsiveness in Japanese quail on developmental instability of female progeny.

Differences in developmental instability were assessed in female offspring of Japanese quail hens selected for reduced (low stress, LS) or exaggerated (high stress, HS) plasma corticosterone (B) response to stress and treated with a placebo or B during egg formation. Hens of each line were implanted (s.c.

Fear responses of offspring from divergent quail stress response line hens treated with corticosterone during egg formation.

Increased fearfulness has been associated with adrenocortical activation. Maternal corticosterone (B) treatment increases egg B, and elevated B in ovo enhances chick avoidance of humans. Quail selected for exaggerated (high stress, HS) rather than reduced (low stress, LS) plasma B response to stress are more fearful, and more B is found in HS hen eggs.

Glucocorticoid exposure alters the pathogenesis of Theiler's murine encephalomyelitis virus during acute infection.

Previous research has shown that chronic restraint stress exacerbates Theiler's virus infection, a murine model for CNS inflammation and multiple sclerosis. The current set of experiments was designed to evaluate the potential role of glucocorticoids in the deleterious effects of restraint stress on acute CNS inflammatory disease. Exposure to chronic restraint stress resulted in elevated levels of corticosterone as well as increased clinical scores and weight loss (Experiment 1).

Ontogeny of the cloacal gland in male Japanese quail classified in a T-maze.

Broiler chicks that traverse a T-maze quickly to reinstate contact with their companions (HP, high performers) are known to grow faster, are more social, and have a reduced plasma corticosterone response to acute stress than slower chicks (LP, low performers). High-performing quail from a line selected for reduced rather than exaggerated (high-stress) adrenocortical stress responsiveness also show enhanced female reproductive performance when compared with LP-high-stress quail. Herein, time courses of male sexual development were evaluated in genetically unremarkable quail that were categorized at 2 d of age as HP or LP in the T-maze.

Cloacal gland growth differences in high and low plasma corticosterone stress response line male quail reared under short daylengths.

Mail quail selected (generation 32) for reduced [low stress (LS)] or exaggerated [high stress (HS)] plasma corticosterone stress response to brief mechanical restraint (5 min of immobilization) were studied for differences in the growth of their cloacal glands when reared essentially lifelong on short day lengths of 8L:16D. Post-brooding, at 4 wk of age, 96 quail (48 LS+48 HS) were housed in cages (1 LS and 1 HS male/cage), and the short day light treatment was instigated. Using a digital caliper, cloacal gland (CG) size measurements (length and width, mm) were made biweekly beginning at 42 d of age and ending at 196 d of age (28 wk old).

Maternal corticosterone further reduces the reproductive function of male offspring hatched from eggs laid by quail hens selected for exaggerated adrenocortical stress responsiveness.

Activation of the hypothalamic-pituitary-adrenal (HPA) axis can depress the hypothalamic-pituitary-testicular axis. Male quail cloacal gland (CG) size and foam production shows androgen dependency, and males selected for exaggerated [high stress (HS)] rather than reduced [low stress (LS)] plasma corticosterone (B) stress response exhibit reduced CG and testes development. High stress hens also deposit more B into egg yolks than LS ones, and quail hens given B produce chicks that have a reduced growth rate and adults with heightened HPA responsiveness.

Active immunization of broiler breeder cockerels against chicken inhibin accelerates puberty and prevents age-induced testicular involution.

Injection of quail and breeder hens with a recombinant protein antigen (MBP-cINA521)--a fusion of the bacterical maltose-binding protein (MBP) and a fragment of the alpha-subunit of chicken inhibin (cINA521)--accelerates puberty and enhances lay. Herein, the effects of this immunogen on reproductive responses in broiler breeder males were assessed. Cockerels were subcutaneously injected with 0 (vehicular controls), 1, 3, or 5 mg of MBP-cINA521 at 13 wk of age and with one-half of these dosages (boosters) at 18 wk.

Cloacal gland and gonadal photoresponsiveness in male Japanese quail selected for divergent plasma corticosterone response to brief restraint.

Corticosterone is linked to testicular depression, and in Coturnix, a relatively photorefractory species, day length and androgen dependency in cloacal gland development and foam production are evident. Furthermore, male quail selected for reduced (low stress, LS) rather than exaggerated (high stress, HS) plasma corticosterone stress response show more photo-induced reproductive development, greater resistance to reproductive declines induced by exposure to very short days, and a quicker recovery to a higher reproductive level upon return to long days. To determine whether a milder reduction in day length would also influence stress line reproductive photoresponsiveness, and perhaps photorefractoriness, males grown on 16 h of light were given 13 h of light for 4 wk followed by a return to long days for 12 wk.

Stressor-induced changes in open-field behavior of Japanese quail selected for contrasting adrenocortical responsiveness to immobilization.

The effect of no treatment (undisturbed controls; CON) or 5 min exposure to immobilization stress (STR) before assessment of open-field behavior in Japanese quail lines selected for high (high stress; HS) or low (low stress; LS) plasma corticosterone (B) response to a similar immobilization stress was examined. During a 5-min test period in an open field, the following behavioral measurements were made: the latency to ambulate (LAMB), the number of open-field floor sectors entered (SECTORS), and freezing behavior (total time spent in complete silence and inactivity, apart from slight movements associated with respiration; FREEZE). A further measure of ambulation across time (ambulation rate; ARATE) was calculated using the formula: ARATE = (SECTORS/[(300 s test ceiling)--LAMB)].

Adrenocortical responses of Japanese quail to a routine weighing procedure and to tonic immobility induction.

Tonic immobility (TI) is induced by brief manual restraint, and it is a commonly used test of fearfulness, particularly in poultry. However, in view of increasing ethical concerns about experimentation on living animals, there is a need to ensure that the tests used do not elicit unacceptable distress. In the United Kingdom, there is some debate as to whether TI should be classified as a regulated experimental procedure that requires a Home Office license to experiment on living animals under the Animals (Scientific Procedures) Act of 1986.

Japanese quail selected for high plasma corticosterone response deposit high levels of corticosterone in their eggs.

Poor habitat quality or body condition often correlates with high responsiveness of the hypothalamo-pituitary-adrenal (HPA) axis rather than with elevated baseline levels of glucocorticoids. We hypothesized that, for egg-laying vertebrates, high responsiveness of the HPA axis would correspond to high concentrations of corticosterone in yolk. We tested the prediction that Japanese quail (Coturnix coturnix japonica) selected for high plasma corticosterone response to brief immobilization (HS quail) would lay eggs with higher yolk corticosterone concentrations than birds selected for low response (LS quail).