Design of dual-labeled oligonucleotide probes for SNPs genotyping.

Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based detection technology or in cell.

Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping.

A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, (Py)U/(2-Ant)U, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the "smart" fluorescently labeled BDF nucleoside, (Py)U/(2-Ant)U, is adenine on the complementary target sequence.

Luminescence properties of dual fluorescent pyrene derivatives used as DNA probe molecule.

We examined the dual fluorescence mechanism of 8-[4-(dimethylamino)phenyl]-N-2-propynyl-1-pyrenecarbox-amide (PyADMA) in polar solvent. The emission band appeared on the short wavelength side does not depend on the solvent polarity. On the other hand, the red shifted emission band depends on solvent polarity.

Synthesis of perylene-labeled base-discriminating fluorescent (BDF) nucleoside and its fluorescence properties.

We have synthesized perylene labeled BDF probe and its photophysical properties were explored. Perylene labeled oligonucleotide probe is capable of detecting cytosine from its target mismatch sequence by enhancement of fluorescence intensity.

Intelligent fluorescent nucleoside in sensing cytosine base: importance of hydrophobic nature of perylene fluorophore.

Fluorescence response upon hybridization of perylene labeled oligonucleotide probes depends on the microenvironment experienced by the perylene fluorophore. In mismatched duplex ((Per)U-C), enhanced fluorescence was observed while in matched duplex ((Per)U-A) fluorescence intensity decreased considerably. This observation will be a promising research effort in giving rise to a new powerful tool in detection of SNP.