Publications by Satish Kumar Kodiripaka

Publications by authors named "Satish Kumar Kodiripaka"

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Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts.

Rituraj Batth Ilenuta Simina Cuciurean Satish Kumar Kodiripaka Sarah Skovlund Rothman Caroline Greisen

Bio Protoc

January 2021

This protocol describes the generation of protoplasts from protonemal tissue of the moss (syn. ), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with enzyme cocktails mix of 1.

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