Publications by authors named "Saskia M Imhoff"
Cell Stem Cell
February 2024
Article Synopsis
- The conjunctival epithelium has two key cell types: goblet cells that produce mucus and keratinocytes that secrete water, with keratinocytes presenting mucins on their surface.
- Research involves long-term organoid cultures of human and mouse conjunctiva, revealing essential gene expression and identification of conjunctival stem cells.
- The study also explores viral infections (HSV1, hAdV8, SARS-CoV-2) in conjunctival cultures, demonstrating treatment options for some infections and documenting gene expression changes induced by these viruses, highlighting the potential for organoid transplantation to study conjunctival health and disease.
Article Synopsis
- The lacrimal gland is crucial for keeping the eye lubricated and protected; problems in its fluid production can lead to dry eye syndrome, causing discomfort and eye damage.
- This research establishes long-term 3D organoid cultures for both mouse and human lacrimal glands, which mimic key features of their natural structure and function.
- The study identifies Pax6 as a critical gene for developing lacrimal gland cells and offers a detailed cell map of human lacrimal tissue, demonstrating that these organoids can replicate tear secretion and even successfully engraft in mouse models.
