Publications by authors named "Sasiprapa Ninwattana"
Background: Sentinel laboratory surveillance for diarrheal disease determined norovirus to be the most common cause of non-bacterial gastroenteritis in people during the COVID-19 pandemic in Thailand. An increase in patients presenting with diarrhea and vomiting in hospitals across Chanthaburi province between December 2021 and January 2022 led to the need for the identification of viral pathogens that may be responsible for the outbreak.
Methods: Fecal samples (rectal swabs or stool) from 93 patients, of which 65 patients were collected during the December 2021 to January 2022 outbreak, were collected and screened for viral infection by real-time RT-PCR.
Article Synopsis
- Omicron emerged as a significant variant of concern in late 2021, highlighted by its rapid spread and unique mutations, prompting a need for better detection methods of viral variants.
- This study evaluated two classification platforms for SARS-CoV-2 variants using 618 COVID-19-positive samples from Bangkok, focusing on Delta, Omicron BA.1, and BA.2 variants.
- The findings showed that Omicron variants BA.1 and BA.2 had 2-3 times higher transmission rates than the Delta variant, offering a cost-effective approach for tracking variants and informing public health decisions.
The rapid emergence of SARS-CoV-2 variants with high severity and transmutability adds further urgency for rapid and multiplex molecular testing to identify the variants. A nucleotide matrix-assisted laser-desorption-ionization time-of-flight mass spectrophotometry (MALDI-TOF MS)-based assay was developed (called point mutation array, PMA) to identify four major SARS-CoV-2 variants of concern (VOCs) including Alpha, Beta, Delta, and Omicron (namely PMA-ABDO) and differentiate Omicron subvariant (namely PMA-Omicron). PMA-ABDO and PMA-Omicron consist of 24 and 28 mutation sites of the spike gene.
View Article and Find Full Text PDFThe rapid emergence of SARS-CoV-2 variants with high severity and transmutability adds further urgency for rapid and multiplex molecular testing to identify the variants. A nucleotide matrix-assisted laser-desorption-ionization time-of-flight mass spectrophotometry (MALDI-TOF MS)-based assay was developed (called point mutation array, PMA) to identify four major SARS-CoV-2 variants of concern (VOCs) including Alpha, Beta, Delta, and Omicron (namely PMA-ABDO) and differentiate Omicron subvariant (namely PMA-Omicron). PMA-ABDO and PMA-Omicron consist of 24 and 28 mutation sites of the spike gene.
View Article and Find Full Text PDFArticle Synopsis
- * Initial antibody levels 12 weeks post-vaccination were insufficient to combat all virus strains, and the effectiveness varied significantly after a single ChAdOx1 booster.
- * The combination of ChAdOx1 and BNT162b2 boosters produced better neutralization response than two ChAdOx1 doses, with two doses of BNT162b2 proving most effective against the Omicron variant.
Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers.
Vaccines (Basel)
April 2022
Inactivated SARS-CoV-2 vaccine (CoronaVac) is commonly used in national immunization programs. However, the immune response significantly declines within a few months. Our study assessed the immune response against SARS-CoV-2 after receiving booster shots of BNT162b2 or ChAdOx1 among health care workers who previously received CoronaVac as their primary immunization.
View Article and Find Full Text PDF