Co-occurrence of SARS-CoV-2 variants in rivers and sewage in India and Brazil.

The genomic monitoring of SARS-CoV-2 variants of concern (VOCs) in riverine and sewage water has been widely used as an epidemiological tool worldwide. But its utility for epidemiological assessments still needs to be evaluated in some areas. Our study encompassed thirteen Brazilian rivers spanning a vast urban expanse across the states of Rio de Janeiro, São Paulo, and Paraná.

Proteomic and metabolic profiling reveals molecular phenotype associated with chemotrophic growth of JA2 on L-tryptophan.

strain JA2 is an anoxygenic phototrophic bacterium, able to grow under different growth modes. Particularly under chemotrophic conditions, it produces novel Trp-melanin, anthocyanin-like, and pyomelanin pigments. However, the underlying molecular adaptations of strain JA2 that lead to the formation of novel metabolites under chemotrophic conditions remain unexplored.

Genomic and functional insights of a mucin foraging Rhodopirellula halodulae sp. nov.

Nine novel strains were obtained from various algal and seagrass samples. The analysis of the 16S rRNA gene-based phylogenetic tree revealed monophyletic placement of all novel strains within the Rhodopirellula genus. The type strain was identified as JC737, which shared 99.

Metagenome-assembled genome of strain JY119.

Here, we report a 22.1-Mbp genome sequence of microcolonial fungi, , isolated from a pine tree bark. The microbiome of the new fungi is predicted to be largely associated with .

Phylogenomic analysis of metagenome-assembled genomes indicates new taxa in the order Spirochaetales and proposal of Thalassospirochaeta sargassi gen. nov. sp. nov. from seaweeds.

Six metagenome-assembled genomes (JB008, JB007, JB015, JB003, JB004, and JB002) belonging to the order Spirochaetales were generated from seaweed samples collected from the Gulf of Mannar, India. The binned genomes JB008 and JB007 shared highest 16S rRNA gene identity of 94.9 % and 92.

, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity.

Axenic cultures of two strains, JC673 and JC717, both belonging to the phylum , were isolated from distinct geographical locations in India. Strain JC673 was obtained from algal mats of a wetland situated in the state of Kerala, India, while strain JC717 originated from the Central Marine Fisheries Research Institute (CMFRI), state of Tamil Nadu, India. The two strains share 99.

Sinomonas cellulolyticus sp. nov., isolated from Loktak lake.

A novel cellulolytic strain JC656 was isolated from the rhizosphere soil of Alisma plantago-aquatica of floating island (Phumdis) of Loktak lake, Manipur, India. The 16S rRNA gene sequence similarities between strain JC656 and other Sinomonas type strains ranged between 98.5 and 97.

Blastopirellula sediminis sp. nov. a new member of Pirellulaceae isolated from the Andaman and Nicobar Islands.

Two cream-coloured strains (JC732, JC733) of Gram-stain negative, mesophilic, catalase and oxidase positive, aerobic bacteria which divide by budding, form crateriform structures, and cell aggregates were isolated from marine habitats of Andaman and Nicobar Islands, India. Both strains had genome size of 7.1 Mb and G + C content of 58.

Draft Genome Sequence of sp. Strain JA880, Isolated from a Salt Pond.

We report the draft genome sequence of sp. strain JA880, which was isolated from a saltwater pond near Pata, Gujarat, India. The genome assembly contains 3,794,364 bp, with a GC content of 63.

Stable isotope-assisted metabolite profiling reveals new insights into L-tryptophan chemotrophic metabolism of Rubrivivax benzoatilyticus.

Anoxygenic photosynthetic bacteria (APB) are metabolically versatile, capable of surviving with an extended range of carbon and nitrogen sources. This group of phototrophic bacteria have remarkable metabolic plasticity in utilizing an array of organic compounds as carbon source/electron donors and nitrogen sources with sophisticated growth modes. Rubrivivax benzoatilyticus JA2 is one such photosynthetic bacterium utilizes L-tryptophan as nitrogen source under phototrophic growth mode and produces an array of indolic compounds of biotechnological significance.

Phylogenomic analysis of the genus : proposal for division of this genus into the emended genus and two novel genera gen. nov. and gen. nov.

The members of the genus are key players in the removal of petroleum hydrocarbons from polluted marine environments. More than half of the species were described in the last decade using 16S rRNA gene phylogeny and genomic-based metrics. However, the 16S rRNA gene identity (<94 %) between some members of the genus suggested their imprecise taxonomic status.

Revisiting the taxonomy of the genus Rhodopirellula with the proposal for reclassification of the genus to Rhodopirellula sensu stricto, Aporhodopirellula gen. nov., Allorhodopirellula gen. nov. and Neorhodopirellula gen. nov.

The current genus Rhodopirellula consists of marine bacteria which belong to the family Pirellulaceae of the phylum Planctomycetota. Members of the genus Rhodopirellula are aerobic, mesophiles and chemoheterotrophs. The here conducted analysis built on 16S rRNA gene sequence and multi-locus sequence analysis based phylogenomic trees suggested that the genus is subdivided into four clades.

Phylogenomic analysis of a metagenome-assembled genome indicates a new taxon of an anoxygenic phototroph bacterium in the family Chromatiaceae and the proposal of "Candidatus Thioaporhodococcus" gen. nov.

In this study, three metagenome-assembled genomes of a sediment sample were constructed. A Bin1 (JB001) genome was identified as a photo-litho-auto/heterotroph (purple sulfur bacteria) bacterium with the ability to fix nitrogen, tolerate salt, and to produce bacteriochlorophyll a. It has a genome length of 4.

Draft Genome Sequence of sp. Strain JC586, Isolated from Soil Sediment of the Floating Islands of Loktak Lake, India.

The 3.52-Mbp whole-genome sequence of a sp. strain isolated from soil sediment of the floating islands of Loktak Lake is reported.

Draft Genome Sequences of the Kocuria subflava Type Strain KCTC 39547 and sp. Strain JC486, a Newly Isolated Strain from a Wild Ass Sanctuary in Gujarat, India.

Here, we report a 2.86-Mbp genome sequence of sp. strain JC486, which was isolated from a salt marsh, and a 3.

Paludisphaera rhizosphaereae sp. nov., a new member of the family Isosphaeraceae, isolated from the rhizosphere soil of Erianthus ravennae.

Two axenic cultures of Planctomycetota were isolated from distinct geographical locations of India. Strain JC665 was isolated from a rhizosphere soil of Loktak lake, Manipur, whereas strain JC747 was isolated from a soil sediment at Pallikkara village, Kerala, India. The two closely related strains shared the highest 16S rRNA gene sequence identity (94.

Neoroseomonas marina sp. nov., Isolated from a Beach Sand.

A pink-pigmented bacterium (strain JC162 = KCTC 32190) was isolated from a beach sand sample. Cells were Gram-stain-negative, coccoid, non-motile, and strictly aerobic. EzBioCloud BLAST search of 16S rRNA gene sequence showed that strain KCTC 32190 had the highest sequence identity to the members of the genus Neoroseomonas and was closely related to N.

A genomic overview including polyphasic taxonomy of Thalassoroseus pseudoceratinae gen. nov., sp. nov. isolated from a marine sponge, Pseudoceratina sp.

A pink-coloured, salt- and alkali-tolerant planctomycetal strain (JC658) with oval to pear-shaped, motile, aerobic, Gram-negative stained cells was isolated from a marine sponge, Pseudoceratina sp. Strain JC658 shares the highest 16S rRNA gene sequence identity with Maioricimonas rarisocia Mal4 (< 89.2%) in the family Planctomycetaceae.

Dynamics of proteo-metabolome from Rubrivivax benzoatilyticus JA2 reveals a programmed switch-off of phototrophic growth, leading to a non-cultivable state as a hyperglycemic effect.

Anoxygenic phototrophic bacteria display phenomenal metabolic plasticity leading to distinct phenotypes. Extracellular elevated glucose levels limit photosynthesis in photosynthetic organisms; diversely, cause oxidative stress with ROS generation and "diabetic" like situation in non-photosynthetic organisms. In this study, longer incubations of externally provided glucose (22 mM) inhibited photosynthetic machinery in a phototrophic bacterium, Rubrivivax benzoatilyticus.

Phylotaxogenomics for the Reappraisal of the Genus With the Creation of Six New Genera.

The genus is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups.

Aquisphaera insulae sp. nov., a new member in the family Isosphaeraceae, isolated from the floating island of Loktak lake and emended description of the genus Aquisphaera.

Strain JC669 was isolated from a floating island of Loktak lake, Manipur, India and shares the highest 16S rRNA gene sequence identity with Aquisphaera giovannonii OJF2. The novel strain is aerobic, Gram negative, light pink-coloured, non-motile, NaCl intolerant and spherical to oval-shaped. It grows in the form of single cells or aggregates and possibly forms structures which appear like fruiting bodies.

Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria.

Our major concern was to address "yeast endobacteria" which was based on a few reports in the recent past where bacteria may find yeast as a niche for survival. In this study, we report the microbiota of twenty-nine axenic yeast cultures recovered from different habitats based on their 16S rRNA gene-amplicon metagenomes. Yeasts were identified based on D1/D2 or ITS gene sequences.

"Candidatus Laterigemmans baculatus" gen. nov. sp. nov., the first representative of rod shaped planctomycetes with lateral budding in the family Pirellulaceae.

Two axenic cultures of Planctomycetes were isolated from distinct geographical regions of the east coast of India. The two closely related strains (JC640 and CH01) showed <93.3% 16S rRNA gene sequence identity with members of the genus Roseimaritima followed by Rhodopirellula (<91%).