Publications by authors named "Sasidhar N Nirudodhi"
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is an established, powerful tool for investigating protein-ligand interactions, protein folding, and protein dynamics. However, HDX-MS is still an emergent tool for quality control of biopharmaceuticals and for establishing dynamic similarity between a biosimilar and an innovator therapeutic. Because industry will conduct quality control and similarity measurements over a product lifetime and in multiple locations, an understanding of HDX-MS reproducibility is critical.
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- A dual protease column combining pepsin and type XIII protease was created to improve the digestion of IgG molecules for hydrogen/deuterium exchange mass spectrometry (HDX-MS).
- The method was specifically optimized for the more challenging IgG2 monoclonal antibodies, but its effectiveness was also demonstrated on IgG1 and IgG4.
- This new approach resulted in better digestion efficiency and sequence coverage, allowing for more detailed analysis of therapeutic IgG molecules, which is important for understanding their structure-function relationships and ensuring clinical safety.