Publications by authors named "Sasha Raskind"
Metabolomic Determinants of Metabolic Risk in Mexican Adolescents.
Obesity (Silver Spring)
September 2017
Objective: The goal of this study was to identify metabolites associated with metabolic risk, separately by sex, in Mexican adolescents.
Methods: Untargeted metabolomic profiling was carried out on fasting serum of 238 youth aged 8 to 14 years, and metabolites associated with a metabolic syndrome risk z-score (MetRisk z-score) were identified separately for boys and girls, using the simulation and extrapolation algorithm. Associations of each metabolite with MetRisk z-score were examined using linear regression models that accounted for maternal education, child's age, and pubertal status.
Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-specific ortholog sequences, profiled gene expression by RNA-seq and small molecules by metabolite profiling, and identified genes and metabolites correlating with species longevity.
View Article and Find Full Text PDFBacterial nutrient foraging in a mouse model of enteral nutrient deprivation: insight into the gut origin of sepsis.
Am J Physiol Gastrointest Liver Physiol
October 2016
Article Synopsis
- Total parenteral nutrition (TPN) alters small intestinal microbiota, mainly increasing Proteobacteria dominance in enterally deprived mice.
- The study utilized liquid chromatography/mass spectrometry (LC/MS) to analyze changes in metabolomic profiles, revealing that TPN mice exhibited amino acid compositions similar to the TPN solution and increased uptake of labeled leucine by their gut bacteria.
- Gavage experiments indicated that transferring contents from TPN mice to germ-free mice prompted a shift towards a pro-inflammatory state, suggesting that TPN dependence compromises epithelial barrier function and promotes dysbiosis.