Publications by authors named "Sasha Gupta"
Article Synopsis
- - Scientists identified specific proteins and molecules in the central nervous system (CNS) that can be targeted to create engineered cells for therapy.
- - They developed synthetic Notch receptors to program T cells to release certain treatments only in the brain, effectively clearing brain tumors without affecting cells in other areas.
- - The research also found that T cells delivering interleukin-10, an immune-suppressing cytokine, helped reduce symptoms in a mouse model of neuroinflammation, showing potential for targeted treatment strategies.
Background: Progressive multifocal leukoencephalopathy (PML) is a frequently fatal disease of the central nervous system caused by JC virus (JCV). Survival is dependent on early diagnosis and ability to re-establish anti-viral T cell immunity. Adoptive transfer of polyomavirus-specific T cells has shown promise; however, there are no readily available HLA-matched anti-viral T cells to facilitate rapid treatment.
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- Experimental autoimmune encephalomyelitis (EAE) is used as a model for studying CNS diseases like multiple sclerosis, and this research focuses on improving the production of recombinant human MOG (rhMOG) to study EAE in mice.
- The new protocol utilizes SHuffle E. coli cells to produce high-yield, soluble rhMOG, overcoming the challenges of using bacterial cells which often lead to insolubility and ineffective protein folding.
- The effective production of rhMOG not only allows for B cell-dependent EAE induction in wild-type mice but also sets the stage for further research into the role of MOG in CNS demyelinating diseases.
CAR-T Cell-Mediated B-Cell Depletion in Central Nervous System Autoimmunity.
Neurol Neuroimmunol Neuroinflamm
March 2023
Background And Objectives: Anti-CD20 monoclonal antibody (mAb) B-cell depletion is a remarkably successful multiple sclerosis (MS) treatment. Chimeric antigen receptor (CAR)-T cells, which target antigens in a non-major histocompatibility complex (MHC)-restricted manner, can penetrate tissues more thoroughly than mAbs. However, a previous study indicated that anti-CD19 CAR-T cells can paradoxically exacerbate experimental autoimmune encephalomyelitis (EAE) disease.
View Article and Find Full Text PDFRemote Observational Research for Multiple Sclerosis: A Natural Experiment.
Neurol Neuroimmunol Neuroinflamm
March 2023
Article Synopsis
- The study investigates the impact of remote evaluations on engagement and costs for participants with chronic neurological conditions like multiple sclerosis (MS), especially due to restrictions from the COVID-19 pandemic.
- Participants engaged in both in-clinic and virtual visits, with a focus on comparing costs and disability assessments between methods.
- Results show that remote evaluations are not only more cost-effective but also yield comparable disability scores to traditional in-clinic assessments, suggesting a shift towards remote monitoring could be beneficial for ongoing research.
Can Systemic Anti-CD20 B Cell-Depleting Antibodies Eliminate Meningeal Follicles in Multiple Sclerosis?
Neurol Neuroimmunol Neuroinflamm
July 2021
Objective: Myasthenia gravis (MG) is an autoimmune condition in which neurotransmission is impaired by binding of autoantibodies to acetylcholine receptors (AChR) or, in a minority of patients, to muscle specific kinase (MuSK). There are differences in the dominant IgG subclass, pathogenic mechanisms, and treatment responses between the two MG subtypes (AChR or MuSK). The antibodies are thought to be T-cell dependent, but the mechanisms underlying their production are not well understood.
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