Publications by authors named "Sascha Roocke"
Article Synopsis
- The study explores how overexpressing FGF23 affects SaOS-2 cells, particularly regarding stress responses and morphology, and its relation to skeletal disorders like X-linked hypophosphatemia (XLH).
- Researchers utilized transmission electron microscopy and protein analysis to observe changes in the rough endoplasmic reticulum (rER) and mitochondria of these cells, noting significant alterations such as enlargement and increased contact zones.
- The findings indicate higher rates of apoptosis and activation of pathways related to cellular stress, suggesting that FGF23 overexpression plays a critical role in how cells manage protein synthesis demands and stress, paving the way for potential therapeutic strategies for related diseases.
Protein sequence databases play a crucial role in the majority of the currently applied mass-spectrometry-based proteomics workflows. Here UniProtKB serves as one of the major sources, as it combines the information of several smaller databases and enriches the entries with additional biological information. For the identification of peptides in a sample by tandem mass spectra, as generated by data-dependent acquisition, protein sequence databases provide the basis for most spectrum identification search engines.
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