Beyond Low Prevalence: Exploring Antibiotic Resistance and Virulence Profiles in Sri Lankan Helicobacter pylori with Comparative Genomics.

infects at least half the population worldwide, and its highly diverse genomic content correlates with its geographic distribution because of its prolonged relationship with humans. The extremely low infection prevalence alongside low inflammation severity observed in some countries might be caused by strains with low virulence potential. Therefore, this study aimed to investigate whole-genome analysis datasets of Sri Lankan strains.

Pathogenomics of Helicobacter pylori.

The human stomach bacterium Helicobacter pylori, the causative agent of gastritis, ulcers and adenocarcinoma, possesses very high genetic diversity. H. pylori has been associated with anatomically modern humans since their origins over 100,000 years ago and has co-evolved with its human host ever since.

Exploring Alternative Treatment Choices for Multidrug-Resistant Clinical Strains of in Mongolia.

is a pathogen related to severe diseases such as gastric cancer; because of rising antimicrobial-resistant strains, failure to eradicate with antibiotics has increased worldwide. Multidrug-resistant and gastric cancer is common in Mongolia; therefore, we aimed to explore alternative antimicrobial treatments and the genomes of resistant strains in this country. A total of 361 strains isolated from patients in Mongolia were considered.

Study of Isolated from a High-Gastric-Cancer-Risk Population: Unveiling the Comprehensive Analysis of Virulence-Associated Genes including Secretion Systems, and Genome-Wide Association Study.

Background: The prevalence of gastric cancer in Mongolia, in East Asia, remains the highest in the world. However, most strains in Mongolia have a less virulent Western-type CagA. We aimed to determine how genomic variation affected gastric diseases, especially gastric cancer, based on comprehensive genome analysis.

Utilization of an Automated Latex Agglutination Turbidity Assay for Assessing Gastric Mucosal Alteration during Infection.

Background/aims: : A latex agglutination turbidity (LA) assay to test for serum antibodies has been approved in Japan and Korea for mass screening of infection. In this study, we evaluated the LA assay for diagnosing infection and predicting gastric mucosal changes in a Mongolian population.

Methods: : In total, 484 individuals were classified into -positive (n=356) and -negative (n=128) groups, as determined by histology and culture.

Global Antimicrobial Resistance Gene Study of : Comparison of Detection Tools, ARG and Efflux Pump Gene Analysis, Worldwide Epidemiological Distribution, and Information Related to the Antimicrobial-Resistant Phenotype.

We conducted a global-scale study to identify antimicrobial-resistant genes (ARG), address their global distribution, and understand their effect on the antimicrobial resistance (AMR) phenotypes of the clinical isolates. We identified ARG using several well-known tools against extensive bacterial ARG databases, then analyzed their correlation with clinical antibiogram data from dozens of patients across countries. This revealed that combining multiple tools and databases, followed by manual selection of ARG from the annotation results, produces more conclusive results than using a single tool or database alone.

Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient.

Helicobacter pylori is involved in the etiology and severity of several gastroduodenal diseases; however, plasticity of the H. pylori genome makes complete genome assembly difficult. We report here the full genomes of H.

Mutations Related to Antibiotics Resistance in Clinical Isolates from Bangladesh.

Current management of gastric inflammation involves the eradication of . However, the effectiveness of commonly used antibiotics against infection has decreased due to antibiotic resistance. Phenotypic-based diagnostics are laborious and finding the cause of resistance can be difficult.

Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis.

Background: 16S rRNA amplicon sequencing is an accurate method of detecting microbial infection without culture. It is unclear if sequencing has additional benefits over routine diagnostic methods for Helicobacter pylori testing.

Methods: We enrolled Mongolian volunteers with dyspepsia.