New Set of EST-STR Markers for Discrimination of Related L. Varieties.

L. is cultivated for its edible seeds and for the production of alkaloids. A serious problem in seed trade and processing is the intentional mixing of excellent food-quality seeds with non-food-grade-quality seeds.

Suspension culture of stem cells established of Calendula officinalis L.

Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allow to consider the establishment of stem cell cultures in a broader range of species. This study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L.

Elicitation of Hyoscyamine Production in L. Plants Using Tobamoviruses.

L. produces tropane alkaloids, and the hyoscyamine is dominant among them. Hyoscyamine is produced by hairy root cultures in vitro derived from native plants or plants with the genetically modified biosynthetic pathway for hyoscyamine.

The Cell-Wall β-d-Glucan in Leaves of Oat ( L.) Affected by Fungal Pathogen f. sp. .

In addition to the structural and storage functions of the (1,3; 1,4)-β-d-glucans (β-d-glucan), the possible protective role of this polymer under biotic stresses is still debated. The aim of this study was to contribute to this hypothesis by analyzing the β-d-glucans content, expression of related cellulose synthase-like () , , genes, content of chlorophylls, and β-1,3-glucanase content in oat ( L.) leaves infected with the commonly occurring oat fungal pathogen, f.

Establishment of Stem Cell-like Cells of (L.) Rusby from Explants Containing Cambial Meristems.

The in vitro cultures of plant stem cells and stem cell-like cells can be established from tissues containing meristematic cells. Chemical compounds-as well as their production potential-is among the emerging topics of plant biotechnology. We induced the callus cell biomass growth and characterized the parameters indicating the presence of stem cells or stem cell-like cells.

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus.

Plant viruses threaten agricultural production by reducing the yield, quality, and economical benefits. Tomato mosaic virus (ToMV) from the genus causes serious losses in the quantity and quality of tomato production. The management of plant protection is very difficult, mainly due to the vector-less transmission of ToMV.

Biotic and Abiotic Elicitors of Stilbenes Production in L. Cell Culture.

The in vitro cell cultures derived from the grapevine ( L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from , the cell wall homogenate from and synthetic jasmonates.

Procedures for DNA Extraction from Opium Poppy ( L.) and Poppy Seed-Containing Products.

Several commonly used extraction procedures and commercial kits were compared for extraction of DNA from opium poppy ( L.) seeds, ground seeds, pollen grains, poppy seed filling from a bakery product, and poppy oil. The newly developed extraction protocol was much simpler, reduced the cost and time required for DNA extraction from the native and ground seeds, and pollen grains.