Regional Gene Therapy Compared to Recombinant BMP-2 for the Treatment of Critical-Size Bone Defects: An In Vivo Single-Cell RNA-Sequencing Study.

regional gene therapy is a promising tissue-engineering strategy for bone regeneration: osteogenic mesenchymal stem cells (MSCs) can be genetically modified to express an osteoinductive stimulus (e.g., bone morphogenetic protein-2), seeded onto an osteoconductive scaffold, and then implanted into a bone defect to exert a therapeutic effect.

Recent Advances in the Development of Greener Methodologies for the Synthesis of Benzothiazoles.

The benzothiazole ring system has been recognised with crucial pharmacophoric features being present among various approved drugs and clinical and pre-clinical candidates. The medicinal importance of this privileged scaffold stimulated the interest of synthetic medicinal/ organic chemists for the synthesis of its derivatives due to their diverse biological applications. In most of the reports in the literature, benzothiazoles were synthesized by cyclocondensation of 2- aminothiophenol with either carboxylic acid and its derivatives or aldehydes.

The Feasibility of Omega-3 Supplementation Compared to Placebo in the Management of Long COVID Symptoms Among Healthcare Workers: A Randomized Controlled Trial.

Background:  COVID-19 is known to cause significant multisystem inflammatory responses, leading to symptoms beyond the acute phase of illness. These "long COVID" symptoms affect quality of life and interfere with daily activities. This pilot study looks at the feasibility, tolerability, and safety of omega-3 (docosahexaenoic acid+eicosapentaenoic acid, EPA) among healthcare workers with long COVID symptoms in New Jersey.

Concurrent targeted delivery of doxorubicin and curcumin to the cancer cells using simple and versatile ligand-installed multifaceted chitosan-based nanoconjugates.

Existing chemotherapeutic approaches against refractory cancers are ineffective due to off-target effects, inefficient delivery, and inadequate accumulation of anticancer drugs at the tumor site, which causes limited efficiency of drug treatment and toxicity to neighboring healthy cells. The development of nano-based drug delivery systems (DDSs) with the goal of delivering desired therapeutic doses to the diseased cells and has already proven to be a promising strategy to address these challenges. Our study focuses on achieving an efficient tumor-targeted delivery of a combination of drugs for therapeutic benefits by developing a versatile DDS by following a simple one-step chemical approach.

Self-sensitized Cu(ii)-complex catalyzed solar driven CO reduction.

Developing a self-sensitized catalyst from earth-abundant elements, capable of efficient light harvesting and electron transfer, is crucial for enhancing the efficacy of CO transformation, a critical step in environmental cleanup and advancing clean energy prospects. Traditional approaches relying on external photosensitizers, comprising 4d/5d metal complexes, involve intermolecular electron transfer, and attachment of photosensitizing arms to the catalyst necessitates intramolecular electron transfer, underscoring the need for a more integrated solution. We report a new Cu(ii) complex, K[CuNDPA] (1[K(18-crown-6)]), bearing a dipyrrin amide-based trianionic tetradentate ligand, NDPA (HL), which is capable of harnessing light energy, despite having a paramagnetic Cu(ii) centre, without any external photosensitizer and photocatalytically reducing CO to CO in acetonitrile : water (19 : 1 v/v) with a TON as high as 1132, a TOF of 566 h and a selectivity of 99%.

Gabapentin as an Adjunctive Therapy in Managing Visceral Hyperalgesia, Severe Feeding Intolerance, and Malnutrition in an Infant: A Case Presentation and Literature Review.

A two-month-old developmentally normal full-term female presented with severe feeding intolerance, progressive weight loss, and persistent fussiness, leading to multiple emergency department visits and eventual hospitalization. Initial evaluations, including laboratory tests and imaging, were unremarkable, prompting a series of diagnostic and therapeutic interventions. A multidisciplinary approach, including empiric gastroesophageal reflux disease (GERD) therapy, was started.

Large-scale evaluation of outcomes after a genetic diagnosis in children with severe developmental disorders.

Purpose: We sought to evaluate outcomes for clinical management after a genetic diagnosis from the Deciphering Developmental Disorders study.

Methods: Individuals in the Deciphering Developmental Disorders study who had a pathogenic/likely pathogenic genotype in the DECIPHER database were selected for inclusion ( = 5010). Clinical notes from regional clinical genetics services notes were reviewed to assess predefined clinical outcomes relating to interventions, prenatal choices, and information provision.

Crafting tailored, well-defined block copolymers of cyclic esters with an organomagnesium initiator.

An organomagnesium complex containing an imino-phosphanamidinate ligand was found to be a competent catalyst for the ROP of -LA and ε-CL as well as their copolymerization sequential addition of monomers, resulting in the formation of PCL--PLA diblock copolymer. The polymers obtained were characterized by H, C, DOSY NMR, DSC, TGA, POM, and SEM.

Functional and Radiological Outcomes of Short-Segment Fixation With Intermediate Screws for Thoracolumbar Spine Fractures.

Introduction Thoracolumbar fractures, particularly burst fractures, represent a significant health concern due to their prevalence and functional impact. This study evaluates the efficacy of short-segment posterior fixation with intermediate screw instrumentation in treating unstable thoracolumbar fractures. Methods A prospective study was conducted from July 2022 to December 2023, including 26 patients with traumatic thoracolumbar fractures.

The proteomic response of to amphotericin B (AmB) reveals the involvement of the RTA-like protein RtaA in AmB resistance.

The polyene antimycotic amphotericin B (AmB) and its liposomal formulation AmBisome belong to the treatment options of invasive aspergillosis caused by . Increasing resistance to AmB in clinical isolates of species is a growing concern, but mechanisms of AmB resistance remain unclear. In this study, we conducted a proteomic analysis of exposed to sublethal concentrations of AmB and AmBisome.

Repurposing of a gill gene regulatory program for outer ear evolution.

How novel structures emerge during evolution has long fascinated biologists. A dramatic example is how the diminutive bones of the mammalian middle ear arose from ancestral fish jawbones. In contrast, the evolutionary origin of the outer ear, another mammalian innovation, remains a mystery, in part because it is supported by non-mineralized elastic cartilage rarely recovered in fossils.

Environmental triggers and ecological implications of a harmful algal bloom in the northern Arabian/Persian Gulf: Insights into the driving forces and consequences.

The Arabian/Persian Gulf, a marginal sea of the northern Indian Ocean, has been significantly impacted by human activities, leading to a rise in harmful algal blooms (HABs). This study investigates the summer blooming of an ichthyotoxic phytoflagellate Chattonella marina var. antiqua and associated fish-kill in Kuwaiti waters, connecting the events to a previous dust storm and eutrophication status in the coastal waters of the Northern Arabian Gulf (NAG).

Monotherapy or combination therapy of Oleanolic acid? From therapeutic significance and drug delivery to clinical studies: A comprehensive review.

Oleanolic acid (OA) is a pentacyclic triterpenoid molecule widely distributed throughout medicinal plants. This naturally occurring OA has attracted considerable interest due to its wide range of pharmacological characteristics, notably its cytotoxic effects on various human cancer cell lines, making it a potential candidate for extensive therapeutic uses. In vivo studies have shown that OA possesses hepatoprotective, cardioprotective, anti-inflammatory and anti-microbial properties.

Physical origin and control of exciton spatial localization in high-MOene monolayers under external perturbations.

Two-dimensional (2D) materials hold great promise for the next-generation optoelectronics applications, many of which, including solar cell, rely on the efficient dissociation of exciton into free charge carriers. However, photoexcitation in atomically thin 2D semiconductors typically produces exciton with a binding energy of ∼500 meV, an order of magnitude larger than thermal energy at room temperature. This inefficient exciton dissociation can limit the efficiency of photovoltaics.

Faculty Development Needs in Southeastern Conference Nursing Schools.

Background: This study examined nurse faculty members' teaching, scholarship, and professional development interests across Southeastern Conference (SEC) nursing schools, and evaluated differences in these needs associated with years in academia, tenure status, faculty rank, and Accountable Health Communities (AHC) versus non-AHC settings.

Method: Rosenthal and Stanberry's framework for faculty development provided the theoretical foundation for this cross-sectional study to explore topics of interests of faculty at 12 SEC nursing schools.

Results: A total of 470 faculty responded to the online survey (57% response rate).

Evaluation of Vulvar Disorders by Vulvoscopy Index and N-S-P Scheme Using Three Rings Vulvoscopy (TRIV).

Background And Objective: TRIV is a new and promising technique based on the fact that Vulva can be divided into three circular zones based on anatomy, embryology, and histology- Outer, Middle, and Inner ring. The Vulvoscopy Index and N-S-P scheme are objective tools to improve the systematization of vulvoscopy findings and to simplify information management using TRIV.

Methods: Hundred women with vulvar complaints (Cases) and 100 asymptomatic women (Controls) underwent TRIV and findings were documented as per N-S-P Scheme and Vulvoscopy Index.

Behind the scenes of EQA - characteristics, capabilities, benefits and assets of external quality assessment (EQA).

This is the first in a series of five papers that detail the role and substantial impact that external quality assessment (EQA) and their providers' services play in ensuring diagnostic (IVD) performance quality. The aim is to give readers and users of EQA services an insight into the processes in EQA, explain to them what happens before EQA samples are delivered and after examination results are submitted to the provider, how they are assessed, what benefits participants can expect, but also who are stakeholders other than participants and what significance do EQA data and assessment results have for them. This first paper presents the history of EQA, insights into legal, financing and ethical matters, information technology used in EQA, structure and lifecycle of EQA programs, frequency and intensity of challenges, and unique requirements of extra-examination and educational EQA programs.

Myths and prestige in Hindu nationalist politics.

Sijilmassi et al. offer a myth-based framework that is particularly useful in understanding the rising tide of Hindu nationalism in contemporary India. We propose that the success of these myths lies partly in drawing upon the evolved human capacity for prestige-based status to induce a sense of belonging and identification with high-prestige Hindu social groups.

Promising antiviral inhibitors against lumpy skin disease: A vetinformatics approach.

Background: Lumpy skin disease (LSD) is a transboundary virus disease that mostly affects cattle. It has recently been reported all over the world, which highlights the need for efficient control methods. LSD poses serious economic dangers worldwide.

Bayesian semiparametric inference in longitudinal metabolomics data.

The article is motivated by an application to the EarlyBird cohort study aiming to explore how anthropometrics and clinical and metabolic processes are associated with obesity and glucose control during childhood. There is interest in inferring the relationship between dynamically changing and high-dimensional metabolites and a longitudinal response. Important aspects of the analysis include the selection of the important set of metabolites and the accommodation of missing data in both response and covariate values.

Pharmacological inactivation of a non-canonical gp130 signaling arm attenuates chronic systemic inflammation and multimorbidity induced by a high-fat diet.

Interleukin-6 (IL-6) is a major pro-inflammatory cytokine that demonstrates a robust correlation with age and body mass index (BMI) as part of the senescence-associated secretory phenotype. IL-6 cytokines also play a crucial role in metabolic homeostasis and regenerative processes primarily via the canonical STAT3 pathway. Thus, selective modulation of IL-6 signaling may offer a unique opportunity for therapeutic interventions.

Persistence of Uterine Artery Doppler Velocimetry Changes in Postpartum Mothers Following a Delivery Complicated by Preeclampsia: A Systematic Review.

In preeclampsia, there occurs a defective trophoblastic invasion of spiral arteries, which is characterized by abnormal uterine artery wave parameter such as increased pulsatility index (PI) and early diastolic notch. This increased uterine artery PI is a good predictor of hypertensive disorder and small for gestational-age babies. Maternal hypertension and proteinuria resolve in the puerperium.