"Rapid diagnosis of Cucumber mosaic virus in banana plants using a fluorescence-based real-time isothermal reverse transcription-recombinase polymerase amplification assay".

Cucumber mosaic virus (CMV) is a widespread plant virus infecting important vegetables, plantation and flower crops. Currently, CMV is detected by enzyme-linked immunosorbent assays (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) assays. ELISA requires polyclonal antibodies and is time-consuming.

Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars.

Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR).

First record of in chilli ( in India.

The occurrence of was confirmed by RT-PCR and partial sequencing of coat protein gene in chilli cultivars Jwalamukhi and Jwalasakhi grown in the experimental farm at IARI, New Delhi. To the best of our knowledge this is the first report of occurrence of cryptovirus in chilli in India.

Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India.

Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Tomato leaf curl New Delhi virus and Squash leaf curl China virus are known to be associated with this disease in India. We were able to identify a third begomovirus-Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus.

Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata in India.

Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata (mungbean isolate, GBNV-MB) was determined and compared with another isolate from Arachis hypogaea (groundnut isolate, GBNV-type). Comparative sequence analysis revealed that the genome organization of the S and M RNA segments of both GBNV-MB and GBNV-type isolates was similar. However, considerable differences were observed in their intergenic regions (IGRs) and the glycoprotein precursors (Gn/Gc) of the M RNA segments.