Computational Fluid Dynamics Turbulence Model and Experimental Study for a Fontan Cavopulmonary Assist Device.

Head-flow HQ curves for a Fontan cavopulmonary assist device (CPAD) were measured using a blood surrogate in a mock circulatory loop and simulated with various computational fluid dynamics (CFD) models. The tests benchmarked the CFD tools for further enhancement of the CPAD design. Recommended Reynolds-Averaged Navier-Stokes (RANS) CFD approaches for the development of conventional ventricular assist devices (VAD) were found to have shortcomings when applied to the Fontan CPAD, which is designed to neutralize off-condition obstruction risks that could contribute to a major adverse event.

Analysis of 6YO pediatric human body model kinematics and kinetics to determine submarining across naturalistic seating postures.

Objectives: The aim of this study was to analyze the kinematics and kinetics of a naturalistically seated 6-year-old (6YO) pediatric human body model and evaluate the metrics described by earlier studies for pediatric ATDs to indicate whether different postures and booster seats were more associated with submarining than others in a frontal impact.

Methods: The PIPER 6YO pediatric human body model was restrained on a lowback (LBB) and a highback (HBB) booster child restraint seat (CRS) in four naturalistic seating postures: leaning-forward, leaning-inboard, leaning-outboard, and a pre-submarining posture, and a baseline reference seating position as per the FMVSS No. 213 protocol.

Effects of morning and evening exposures to blue light of varying illuminance on ocular growth rates and ocular rhythms in chicks.

Recent evidence indicates that moderate levels of blue light are sufficient to suppress the nighttime rise in serum melatonin in humans, suggesting that luminous screens may be deleterious to sleep cycles and to other functions. Little is known however, about the effects of exposures to blue light on ocular physiology. We tested the effects of transient blue light exposures of various illuminances on ocular growth rates and ocular rhythms in chicks.

In-depth analysis of crash contributing factors and potential ADAS interventions among at-risk drivers using the SHRP 2 naturalistic driving study.

Objective: Motor vehicle crashes remain a significant problem. Advanced driver assistance systems (ADAS) have the potential to reduce crash incidence and severity, but their optimization requires a comprehensive understanding of driver-specific errors and environmental hazards in real-world crash scenarios. Therefore, the objectives of this study were to quantify contributing factors using the Strategic Highway Research Program 2 (SHRP 2) Naturalistic Driving Study (NDS), identify potential ADAS interventions, and make suggestions to optimize ADAS for real-world crash scenarios.

Analysis of Kinematic Response of Pediatric Occupants Seated in Naturalistic Positions in Simulated Frontal Small Offset Impacts: With and Without Automatic Emergency Braking.

Naturalistic driving studies have shown that pediatric occupants do not assume ideal seating positions in real-world scenarios. Current vehicle assessment programs and child restraint system (CRS) sled tests, such as FMVSS No. 213, do not account for a wide range of seating postures that are typically observed during real-world trips.

Pediatric occupant human body model kinematic and kinetic response variation to changes in seating posture in simulated frontal impacts - with and without automatic emergency braking.

Objective: The study quantifies the kinematics of children in booster child restraint systems (CRSs) in various naturalistic seating postures exposed to frontal impacts in a full-vehicle environment, with and without the application of pre-crash automatic emergency braking.

Methods: The PIPER 6YO and 10YO pediatric human body models were positioned in CRSs. The 6YO was restrained on a lowback (LBB) and highback (HBB) booster, while the 10YO was positioned on an LBB and in a NoCRS condition.

Visual conditions affecting eye growth alter diurnal levels of vitreous DOPAC.

In chicks, the diurnal patterns of retinal dopamine synthesis and release are associated with refractive development. To assess the within-day patterns of dopamine release, we assayed vitreal levels of DOPAC (3,4-dihydroxyphenylacetic acid) using high performance liquid chromatography with electrochemical detection, at 4-h intervals over 24 h in eyes with experimental manipulations that change ocular growth rates. Chicks were reared under a 12 h light/12 h dark cycle; experiments began at 12 days of age.

Visual Image Quality Impacts Circadian Rhythm-Related Gene Expression in Retina and in Choroid: A Potential Mechanism for Ametropias.

Purpose: Stimulated by evidence implicating diurnal/circadian rhythms and light in refractive development, we studied the expression over 24 hours of selected clock and circadian rhythm-related genes in retina/retinal pigment epithelium (RPE) and choroid of experimental ametropias in chicks.

Methods: Newly hatched chicks, entrained to a 12-hour light/dark cycle for 12 to 14 days, either experienced nonrestricted vision OU (i.e.

The effects of brief high intensity light on ocular growth in chicks developing myopia vary with time of day.

Evidence suggests that the relevant variable in the anti-myopigenic effect of increased time spent outdoors is the increase in light intensity. Because light is the strongest Zeitgeber, it is plausible that the effects of bright light exposure depend on time of day, and may impact circadian rhythms. In these studies, we asked whether the effects on eye growth rates and ocular rhythms of brief daily exposures to bright light differed depending on time of day in eyes developing myopia in response to form deprivation (FD) or negative lens-induced hyperopic defocus (LENS).

Responses of the scaled pediatric human body model in the rear- and forward-facing child seats in simulated frontal motor vehicle crashes.

The study presents the first-ever endeavor at developing 18-, 24-, 30-, 36-, 42-, and 48-month-old pediatric finite element models from the 6-year-old PIPER human body model as a baseline and comparing their responses systematically in rear-facing and forward-facing simulations across similar boundary conditions. A 6-year-old PIPER model was scaled down to create anthropometric models of the 18-, 24-, 30-, 36-, 42-, and 48-month-old child using the PIPER scaling tool. The models were installed on a convertible car seat (rear-facing and forward-facing configurations) installed with a 3-point lap-shoulder belt in the rear outboard seat of a 2012 Toyota Camry vehicle model finite element model and setup for full-frontal crash simulation (24 , 120 ms pulse).

Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration.

Recessive Stargardt disease (STGD1) is an inherited blinding disorder caused by mutations in the gene. ABCA4 is a flippase in photoreceptor outer segments (OS) that translocates retinaldehyde conjugated to phosphatidylethanolamine across OS disc membranes. Loss of ABCA4 in mice and STGD1 patients causes buildup of lipofuscin in the retinal pigment epithelium (RPE) and degeneration of photoreceptors, leading to blindness.

Evaluating the response of the PIPER scalable human body model across child restraining seats in simulated frontal crashes.

Objective: Booster seats ensure appropriate belt fit for children that a traditional vehicle seat belt cannot offer to small occupants. In this study, the responses of the PIPER 6-year-old human body model are compared to the traditional Q6 anthropomorphic test dummy (ATD).

Methods: Eight frontal impact finite element simulations were run using 4 different child restraining systems on the FMVSS 213 test bench.

Complement modulation in the retinal pigment epithelium rescues photoreceptor degeneration in a mouse model of Stargardt disease.

Recessive Stargardt macular degeneration (STGD1) is caused by mutations in the gene for the ABCA4 transporter in photoreceptor outer segments. STGD1 patients and (STGD1) mice exhibit buildup of bisretinoid-containing lipofuscin pigments in the retinal pigment epithelium (RPE), increased oxidative stress, augmented complement activation and slow degeneration of photoreceptors. A reduction in complement negative regulatory proteins (CRPs), possibly owing to bisretinoid accumulation, may be responsible for the increased complement activation seen on the RPE of STGD1 mice.

Biocompatibility of a Synthetic Biopolymer for the Treatment of Rhegmatogenous Retinal Detachment.

Objective: The aim of this study is to evaluate the retinal safety and toxicity of a novel synthetic biopolymer to be used as a patch to treat rhegmatogenous retinal detachment.

Methods: Thirty one adult wild type albino mice were divided in 2 groups. In Group A (n=9) 0.

Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina.

Background: The rod photoreceptor cGMP-gated cation channel, consisting of three α- and one β subunit, controls ion flow into the rod outer segment (ROS). In addition to the β-subunit, the Cngb1 locus encodes an abundant soluble protein, GARP2 that binds stoichiometrically to rod photoreceptor cGMP phosphodiesterase type 6 (PDE6). To examine the in vivo functional role of GARP2 we generated opsin promoter-driven transgenic mice overexpressing GARP2 three-fold specifically in rod photoreceptors.

Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina.

Absorption of a light particle by an opsin-pigment causes photoisomerization of its retinaldehyde chromophore. Restoration of light sensitivity to the resulting apo-opsin requires chemical re-isomerization of the photobleached chromophore. This is carried out by a multistep enzyme pathway called the visual cycle.

Knockout of GARPs and the β-subunit of the rod cGMP-gated channel disrupts disk morphogenesis and rod outer segment structural integrity.

Ion flow into the rod photoreceptor outer segment (ROS) is regulated by a member of the cyclic-nucleotide-gated cation-channel family; this channel consists of two subunit types, alpha and beta. In the rod cells, the Cngb1 locus encodes the channel beta-subunit and two related glutamic-acid-rich proteins (GARPs). Despite intensive research, it is still unclear why the beta-subunit and GARPs are coexpressed and what function these proteins serve.

The ups and downs of peer review.

This article traces the history of peer review of scientific publications, plotting the development of the process from its inception to its present-day application. We discuss the merits of peer review and its weaknesses, both perceived and real, as well as the practicalities of several major proposed changes to the system. It is our hope that readers will gain a better appreciation of the complexities of the process and, when serving as reviewers themselves, will do so in a manner that will enhance the utility of the exercise.

The Drosophila ninaG oxidoreductase acts in visual pigment chromophore production.

The Drosophila ninaG mutant is characterized by low levels of Rh1 rhodopsin, because of the inability to transport this rhodopsin from the endoplasmic reticulum to the rhabdomere. ninaG mutants do not affect the biogenesis of the minor opsins Rh4 and Rh6. A genetic analysis placed the ninaG gene within the 86E4-86E6 chromosomal region.