Platelets modulate endothelial cell response to dynamic shear stress through PECAM-1.

Introduction: Both vascular endothelial cells and platelets are sensitive to blood flow induced shear stress. We have recently reported that platelet-endothelial cell interaction could greatly affect platelet activation under flow. In the present study, we aimed to investigate how platelet-endothelial cell interaction affected endothelial cell inflammatory responses under flow.

Effects of physiologically relevant dynamic shear stress on platelet complement activation.

Disturbed shear stress, commonly found in cardiovascular diseases, plays important roles in platelet activation and functions. It has been reported that when activated by elevated shear stress, platelets were able to support complement activation to completion. In this study, through a dynamic cone and plate shearing device, three physiologically relevant shear stresses were applied to platelets, mimicking the shear conditions when platelets pass through a normal left coronary artery (0.

The effect of physiologically relevant dynamic shear stress on platelet and endothelial cell activation.

Introduction: Blood flow induced shear stress plays an important role in platelet and endothelial cell functions. The goal of this study was to investigate the effect of physiologically relevant dynamic shear stress on platelet and endothelial cells.

Materials And Methods: Pulsatile shear stress waveforms mimicking the flow in a normal left coronary artery (0.

Effect of geometrical assumptions on numerical modeling of coronary blood flow under normal and disease conditions.

Shear stress plays a pivotal role in pathogenesis of coronary heart disease. The spatial and temporal variation in hemodynamics of blood flow, especially shear stress, is dominated by the vessel geometry. The goal of the present study was to investigate the effect of 2D and 3D geometries on the numerical modeling of coronary blood flow and shear stress distribution.

3D numerical simulation of coronary blood flow and its effect on endothelial cell activation.

The goal of the present study was to develop a physiologically realistic 3D computational fluid dynamics (CFD) model of the left coronary artery under normal and disease conditions to estimate blood flow induced shear stress, and then use the computed shear stress to stimulate vascular endothelial cells in vitro. A 3D geometry of the left coronary artery was built in ProE and the CFD analysis of the flow field was carried out in Fluent (v 6.23) under normal, 30%, 60% and 80% stenosis conditions.