Pluronic F-127 coated zinc oxide nanoparticles from : a comprehensive assessment of antibacterial, and anticancer activities.

The study synthesized Pluronic F-127 coated zinc oxide nanoparticles (PF-127ZnO NPs) using leaf extract and evaluated their antibacterial, antioxidant, and anticancer properties using various characterization methods such as UV-Vis, Fourier Transform Infra-Red, Photoluminescence spectroscopy, FESEM, EDAX, TEM, SAED, XRD, and DLS. The disc diffusion technique was used to evaluate the antibacterial properties of synthesized Pluronic F-127 coated zinc oxide nanoparticles against various pathogens and significant anticancer activity was noticed. The study examined the cytotoxicity of PF127ZnO nanoparticles to Hep3B cells .

Molecular typing of dengue virus in Mizoram, Northeast India.

Introduction: Dengue is an emerging vector-borne public health threat and characterization at the molecular level is important for proper management of the disease. The aim of the study is to examine the diversity of the dengue viral serotypes from a hilly mountainous region of Northeast India.

Methodology: Thirty-six blood samples that were positive for dengue virus IgM antibodies identified by the enzyme-linked immunosorbent assay (ELISA) method were collected and quantified for the IL6 gene expression by using reverse transcriptase polymerase chain reaction (RT-PCR).

Transcriptome analysis reveals SALL4 as a prognostic key gene in gastric adenocarcinoma.

Background: Stomach adenocarcinoma (STAD) dominates 80-90% of gastric cancer (GC). Over the years, it has been realized that the identification of the genes responsible for gastric carcinogenesis is essential to understand the biomarker discovery.

Methods: This study aims to identify candidate genes for biomarker discovery in STAD.

Molecular dynamics simulation, synthesis and topoisomerase inhibitory actions of vanillin derivatives: a systematic computational structural integument.

A series of 4-hydroxy-3-methoxy benzaldehyde (vanillin) derivatives () was designed for the principle of Schiff base condensation with several individual sulfanilamide analogues. The inhibitory potencies of the designed compounds were evaluated through molecular docking simulation studies against the targets, breast cancer-topo isomerase-IIα and estrogen receptor-α; and the top scoring poses with higher binding energy were selected to assess the mode of binding and stability of each complex through molecular dynamics simulations. Compounds that remained stable in the active sites of the both target receptors through a number of strong H-bonds and hydrophobic contacts were selected.

Fish gill microbiome from India's largest Brahmaputra River-a trans-border biodiversity hotspot region.

In this study, we sequenced the V3-V4 region of 16S rRNA gene amplicon using paired-end Illumina HiSeq to study the bacterial community in the gills of fish from the bank of the trans-border river of Brahmaputra, Northeast India. Metagenome data consisted of 278,784 reads, 248-bp length, and 56.48% GC content with 85% sequence having a Phred score Q = 30.

Biosurfactants and anti-inflammatory activity: A potential new approach towards COVID-19.

Coronavirus disease 2019 (COVID-19) has grown to be global public health emergency. The biosurfactants (BSs) are surface-active biomolecules with unique properties and wide applications. Several microbes synthesize secondary metabolites with surface-active properties, which have a wide range of anti-inflammatory and anti-viral roles.

DNA binding activity and molecular docking reveals pierisin-5 as an anti-proliferative agent against gastric cancer.

Pierisin-5 is a DNA dependent ADP ribosyltransferase (ADRT) protein from the larvae of Indian cabbage white butterfly, Interestingly, Pierisin-5 ADP-ribosylates the DNA as a substrate, but not the protein and subsequently persuades apoptotic cell death in human cancer cells. This has led to the investigation on the DNA binding activity of Pierisin-5 using and approaches in the present study. However, both the structure and the mechanism of ADP-ribosylation of pierisin-5 are unknown.

Influence of Micronutrients on the Food Consumption Rate and Silk Production of (Lepidoptera: Bombycidae) Reared on Mulberry Plants Grown in a Mountainous Agro-Ecological Condition.

The study involves analyzing the performance of bivoltine larvae reared on different host plants varieties. The consumption rate (CR) of different strains of was high when fed with Jorhat and TR10 mulberry plant varieties. Jorhat and TR10 mulberry plant varieties were found to contain significant amount of calcium, potassium, magnesium and phosphorus.

Recombinant Pierisin-5 Induces Apoptosis and Differential Expression of Bcl-2, Bax, and p53 in Human Cancer Cells.

Pierisin-5 protein (pie-5) belongs to a family of proteins possessing DNA-dependent ADP-ribosyltransferase activity, which can induce apoptotic cell death. The baculovirus-mediated expression vector system (BEVS) has been commonly used for expression of heterologous protein subunits for basic scientific research, in addition to the development and production of diagnostics and vaccines. In this study, a new method for the expression of the cytotoxic protein was established using the baculovirus expression system.

Induction of Apoptosis by Pierisin-6 in HPV Positive HeLa and HepG2 Cancer Cells is Mediated by the Caspase-3 Dependent Mitochondrial Pathway.

Background: To explore the cytotoxic and apoptotic activity of the pierisin-6 protein in HPV HeLa and HepG2 cell lines.

Methods: In this study, isolation, and purification of cytotoxic Prierisin-6 from the larvae of Pieris napi by affinity column chromatography techniques. Characterization of full-length mRNA of pierisin-6 gene was performed using 3'/5' RACE PCR.

Pharmacological potential of Bidens pilosa L. and determination of bioactive compounds using UHPLC-QqQ-MS/MS and GC/MS.

Background: Research of natural products from traditionally used medicinal plants to fight against the human ailments is fetching attention of researchers worldwide. Bidens pilosa Linn. var.

Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India.

Plants have been used since ancient times as an important source of biologically active substances. The aim of the present study was to investigate the phytochemical constituents (flavonoids and phenolics), antioxidant potential, cytotoxicity against HepG2 (human hepato carcinoma) cancer cell lines, and the antimicrobial activity of the methanol extract of selected traditional medicinal plants collected from Mizoram, India. A number of phenolic compounds were detected using HPLC-DAD-ESI-TOF-MS, mainly Luteolin, Kaempferol, Myricetin, Gallic Acid, Quercetin and Rutin, some of which have been described for the first time in the selected plants.

Characterization and cytotoxic activity of apoptosis-inducing pierisin-5 protein from white cabbage butterfly.

In this study, caspase-dependent apoptosis-inducing pierisin-5 gene was identified and characterized from cabbage white butterfly, Pieris canidia. A thousand-fold increase in expression of pierisin-5 gene was observed from second to third instar larvae, gradually decreasing before pupation. Pierisin-5 was purified from the fifth-instar larvae and was found to exhibit cytotoxicity against HeLa and HepG2 human cancer cell lines.