Suppressive effects of toll-like receptor 2, toll-like receptor 4, and toll-like receptor 7 on protective responses to Mycobacterium bovis BCG from epithelial cells.

Mycobacteria have several mechanisms for evasion of protective responses mounted by the host. In this study, we unravel yet another mechanism that is mediated by Toll-Like Receptors TLR2, TLR4, and TLR7 in epithelial cells. We show that mycobacterial infection of epithelial cells increases the expression of TLR2, TLR4, and TLR7.

Renal fibroblasts are involved in fibrogenic changes in kidney fibrosis associated with dysfunctional telomeres.

Tubulointerstitial fibrosis associated with chronic kidney disease (CKD) represents a global health care problem. We previously reported that short and dysfunctional telomeres lead to interstitial renal fibrosis; however, the cell-of-origin of kidney fibrosis associated with telomere dysfunction is currently unknown. We induced telomere dysfunction by deleting the Trf1 gene encoding a telomere-binding factor specifically in renal fibroblasts in both short-term and long-term life-long experiments in mice to identify the role of fibroblasts in renal fibrosis.

Potassium ion channel Kir2.1 negatively regulates protective responses to Mycobacterium bovis BCG.

Tuberculosis caused by the pathogen Mycobacterium tuberculosis leads to increased mortality and morbidity worldwide. The prevalence of highly drug-resistant strains has reinforced the need for greater understanding of host-pathogen interactions at the cellular and molecular levels. Our previous work demonstrated critical roles of calcium ion channels in regulating protective responses to mycobacteria.

Management of Dentally Anxious Adolescents with Retraining Technique: A Double-blind Randomized Controlled Clinical Study.

Background: Anxiety toward dental treatment may be the reason for not only young children but also secondary school children to postpone dental treatment despite having severe pain. Hence this study was undertaken to recognize such anxious secondary school children prior to the treatment and tried to manage them with the retraining technique.

Materials And Methodology: The present interventional study comprised 100 participants with highly anxious about dental treatment and were selected randomly within the secondary school age group of 11-16 years visiting the dental hospital.

Mycobacteria modulate SUMOylation to suppresses protective responses in dendritic cells.

Post translational modifications (PTMs) are exploited by various pathogens in order to escape host immune responses. SUMOylation is one of the PTMs which is involved in regulation of a variety of cellular responses. However, the effects of host SUMOylation on pathogenic bacteria largely remain elusive.

HSP-27 and HSP-70 negatively regulate protective defence responses from macrophages during mycobacterial infection.

Mycobacterium tuberculosis attenuates many defence responses from alveolar macrophages to create a niche at sites of infection in the human lung. Levels of Heat Shock Proteins have been reported to increase many folds in the serum of active TB patients than in latently infected individuals. Here we investigated the regulation of key defence responses by HSPs during mycobacterial infection.

Identification and characterization of antimicrobial peptide (AMP) candidate from Gonggong Sea Snail () extract.

This study aimed to explore the potency of Gonggong sea snail's (GSS) extract as an antimicrobial peptide (AMP) source. The results showed that the GSS meat extracts exhibited potential antimicrobial activity against and . A peptide band with a molecular weight < 5 kDa was obtained for the characterization of AMP candidates after separating the selected extract using SDS-PAGE, and the sequences were acquired by LC-ESI-MS analysis.

Consequences of telomere dysfunction in fibroblasts, club and basal cells for lung fibrosis development.

TRF1 is an essential component of the telomeric protective complex or shelterin. We previously showed that dysfunctional telomeres in alveolar type II (ATII) cells lead to interstitial lung fibrosis. Here, we study the lung pathologies upon telomere dysfunction in fibroblasts, club and basal cells.

A Study to Assess Patients' Perception and Acceptance of Teledentistry for Care During the Covid-19 Pandemic.

Background: With the aim of preventing the spread of coronavirus disease 2019 (COVID-19), teledentistry allows for the follow-up of patients who can be remotely monitored, decreasing patient mobilization and reducing the risk of virus dissemination. The aim of this cross-sectional pilot survey was to assess patients' perception and acceptance of a teledentistry program during the COVID-19 pandemic.

Materials And Methods: Demographic characteristics of the patients were recorded, and a 24-question survey was completed.

An Comparative Study of the Soft Tissue Response and Esthetics of the Titanium Implant with Titanium Collar by Flapless and Conventional Flap Technique.

Background: These days, patients want quick results for tooth replacement and esthetic results. However, there is no direct correlation between the achievement of osseointegration and the outcome of successful treatment always. It is vital to sustaining peri-implant soft tissue health for extensive tenure success of the implant.

Modified Conventional Root Canal Shaping Technique in Primary Teeth: An Study.

Aim And Objective: The purpose of the study was to compare the efficiency of modified conventional root canal shaping technique versus conventional shaping technique in primary teeth.

Materials And Methods: A total of 184 primary root canals (2 groups of 92 canals each) with two-thirds of root length were selected and randomly allocated into two different shaping techniques, i.e.

Brain Metabolite, Myo-inositol, Inhibits Catalase Activity: A Mechanism of the Distortion of the Antioxidant Defense System in Alzheimer's disease.

A strong correlation between brain metabolite accumulation and oxidative stress has been observed in Alzheimer's disease (AD) patients. There are two central hypotheses for this correlation: (i) coaccumulation of toxic amyloid-β and Myo-inositol (MI), a significant brain metabolite, during presymptomatic stages of AD, and (ii) enhanced expression of MI transporter in brain cells during oxidative stress-induced volume changes in the brain. Identifying specific interactive effects of MI with cellular antioxidant enzymes would represent an essential step in understanding the oxidative stress-induced AD pathogenicity.

EVALUATION OF INAPPROPRIATE PRESCRIPTIONS IN THE GERIATRIC POPULATION OF AN URBAN SLUM IN BANGALORE.

Although the over 60 years age population constitutes approximately 8% of the total population, 30% of total medication is prescribed for this age group. Chronic diseases and polypharmacy in the elderly population increase the risk of inappropriate drug use. Some physicians prescribe medications without reviewing other supplements and medications used by the elder patient.

Loss of ASAP1 in the MMTV-PyMT model of luminal breast cancer activates AKT, accelerates tumorigenesis, and promotes metastasis.

ASAP1 is a multi-domain adaptor protein that regulates cytoskeletal dynamics, receptor recycling and intracellular vesicle trafficking. Its expression is associated with poor prognosis in a variety of cancers, and can promote cell migration, invasion and metastasis. Although amplification and expression of ASAP1 has been associated with poor survival in breast cancer, we found that in the autochthonous MMTV-PyMT model of luminal breast cancer, ablation of ASAP1 resulted in an earlier onset of tumor initiation and increased metastasis.

Traumatic Ulcerations Frequencies and Postinsertion Adjustment Appointments in Complete Denture Patients.

Background And Aim: The patient who is wearing a denture after missing teeth faces traumatic ulceration very frequently. This ulceration creates difficulties in denture wearing. Hence, this study aimed to evaluate the most common locations of traumatic injuries in form of ulcerations, their frequency, and also the duration and number of adjustment visits required to achieve patient comfort following placement of complete removable dentures.

Bcl2 negatively regulates Protective Immune Responses During Infection.

We previously reported that on its own as well as together with HIV inhibits macrophage apoptosis by upregulating the expression of Bcl2 and Inhibitor of Apoptosis (IAP). In addition, recent reports from our lab showed that stimulation of either macrophages or BMDCs results in the significant upregulation of Bcl2. In this report, we delineate the role of Bcl2 in mediating defense responses from dendritic cells (BMDCs) during mycobacterial infection.

Short and dysfunctional telomeres sensitize the kidneys to develop fibrosis.

Accumulation of short telomeres is a hallmark of aging. Mutations in telomerase or telomere-binding proteins lead to telomere shortening or dysfunction and are at the origin of human pathologies known as 'telomere syndromes', which are characterized by loss of the regenerative capacity of tissues and fibrotic pathologies. Here, we generated two mouse models of kidney fibrosis, either by combining telomerase deficiency to induce telomere shortening and a low dose of folic acid, or by conditionally deleting Trf1, a component of the shelterin telomere protective complex, from the kidneys.

Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.

Nearly 6 million Americans suffer from heart failure. Increased fibrosis contributes to functional decline of the heart that leads to heart failure. Previously, we identified a mechanosensitive protein, small proline-rich repeat 3 (SPRR3), in vascular smooth muscle cells of atheromas.

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts.

Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that mitigate fibrosis. However, fibroblast research has been hindered due to the lack of universally acceptable fibroblast markers to identify quiescent as well as activated fibroblasts.

The mTOR pathway is necessary for survival of mice with short telomeres.

Telomerase deficiency leads to age-related diseases and shorter lifespans. Inhibition of the mechanistic target of rapamycin (mTOR) delays aging and age-related pathologies. Here, we show that telomerase deficient mice with short telomeres (G2-Terc) have an hyper-activated mTOR pathway with increased levels of phosphorylated ribosomal S6 protein in liver, skeletal muscle and heart, a target of mTORC1.

Phloretin attenuates STAT-3 activity and overcomes sorafenib resistance targeting SHP-1-mediated inhibition of STAT3 and Akt/VEGFR2 pathway in hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy. Phloretin (PH) possesses anticancer, antitumor, and hepatoprotective effects, however, the effects and potential mechanisms of phloretin remain elusive.

Methods: Five HCC cells were tested in vitro for sensitivity to PH, Sorafenib (Sor) or both and the apoptosis, signal transduction and phosphatase activity were analyzed.