Principle of DNA recognition by sporulation-regulatory protein (Spo0A) in .

Master sporulation-regulatory protein Spo0A binds to the cognate DNA sequence 5'-TGTCGAA-3' (0 A-box) and control transcription of hundreds of genes in sporulating . Thus, discrimination of similar near-cognate 0 A-box sequences (differing by a single base pair) by Spo0A is critical for accurate transcriptional control. The thermodynamics underlying the 0 A-box recognition by Spo0A is unknown.

Optimization of DNA extraction from seeds and leaf tissues of Chrysanthemum (Chrysanthemum indicum) for polymerase chain reaction.

Chrysanthemums constitute approximately 30 species of perennial flowering plants, belonging to the family Asteraceae, native to Asia and Northeastern Europe. Chrysanthemum is a natural cosmetic additive extracted from Chinese herb by modern biochemical technology. It has the properties of anti-bacterial, anti-viral, reducing (detoxification) and anti-inflammation.