Ca²⁺ signaling in myenteric interstitial cells of Cajal (ICC-MY) and their role as conditional pacemakers in the colon.

Interstitial cells of Cajal in the plane of the myenteric plexus (ICC-MY) serve as electrical pacemakers in the stomach and small intestine. A similar population of cells is found in the colon, but these cells do not appear to generate regular slow wave potentials, as characteristic in more proximal gut regions. Ca handling mechanisms in ICC-MY of the mouse proximal colon were studied using confocal imaging of muscles from animals expressing GCaMP6f exclusively in ICC.

Software for segmenting and quantifying calcium signals using multi-scale generative adversarial networks.

Cellular calcium fluorescence imaging utilized to study cellular behaviors typically results in large datasets and a profound need for standardized and accurate analysis methods. Here, we describe open-source software (4SM) to overcome these limitations using an automated machine learning pipeline for subcellular calcium signal segmentation of spatiotemporal maps. The primary use of 4SM is to analyze spatiotemporal maps of calcium activities within cells or across multiple cells.

New open-source software for subcellular segmentation and analysis of spatiotemporal fluorescence signals using deep learning.

Cellular imaging instrumentation advancements as well as readily available optogenetic and fluorescence sensors have yielded a profound need for fast, accurate, and standardized analysis. Deep-learning architectures have revolutionized the field of biomedical image analysis and have achieved state-of-the-art accuracy. Despite these advancements, deep learning architectures for the segmentation of subcellular fluorescence signals is lacking.