Identification of Peptide Mimotope Ligands for Natalizumab.

Mimotope peptides selected from combinatorial peptide libraries can be used as capture reagents for immunoassay detection of therapeutic monoclonal antibodies (mAbs). We report the use of phage display libraries to identify peptide ligands (Veritopes) that bind natalizumab, a therapeutic mAb indicated for use in multiple sclerosis. PKNPSKF is identified as a novel natalizumab-binding motif, and peptides containing this motif demonstrated utility as capture reagents in enzyme-linked immunosorbent assays (ELISAs).

Electrochemical monitoring of native catalase activity in skin using skin covered oxygen electrode.

A skin covered oxygen electrode, SCOE, was constructed with the aim to study the enzyme catalase, which is part of the biological antioxidative system present in skin. The electrode was exposed to different concentrations of HO and the amperometric current response was recorded. The observed current is due to HO penetration through the outermost skin barrier (referred to as the stratum corneum, SC) and subsequent catalytic generation of O by catalase present in the underlying viable epidermis and dermis.