Classifying changes in LN-18 glial cell morphology: a supervised machine learning approach to analyzing cell microscopy data via FIJI and WEKA.

In cell-based research, the process of visually monitoring cells generates large image datasets that need to be evaluated for quantifiable information in order to track the effectiveness of treatments in vitro. With the traditional, end-point assay-based approach being error-prone, and existing computational approaches being complex, we tested existing machine learning frameworks to find methods that are relatively simple, yet powerful enough to accomplish the goal of analyzing cell microscopy data. This paper details the machine learning pipeline for pixel-based classification and object-based classification.