Publications by authors named "Sarah Kuschert"
Peptides and proteins containing non-canonical amino acids (ncAAs) are a large and important class of biopolymers. They include non-ribosomally synthesised peptides, post-translationally modified proteins, expressed or synthesised proteins containing unnatural amino acids, and peptides and proteins that are chemically modified. Here, we describe a general procedure for generating atomic descriptions required to incorporate ncAAs within popular NMR structure determination software such as CYANA, CNS, Xplor-NIH and ARIA.
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- Peptides that target specific protein interactions present a promising therapy option, bridging the gap between small molecules and therapeutic proteins, but they face challenges like poor stability and low permeability.
- Cyclization of peptides, including techniques like "stapling," helps to create more stable and permeable cyclic peptides by enhancing their structure and resistance to metabolism.
- Through a detailed analysis of various cyclization methods, the study identifies effective cyclic peptides that target Mint2, with all-hydrocarbon stapling significantly boosting their stability and cell permeability.