Publications by authors named "Sarah Kurth"
Inflammation models with the proinflammatory cytokine interleukin-1β (IL-1β) are widely used in the in vitro investigation of new therapeutic approaches for osteoarthritis (OA). The aim of this study was to systematically analyze the influence of IL-1β in a 3D chondral pellet culture model. Bovine articular chondrocytes were cultured to passage 3 and then placed in pellet culture.
View Article and Find Full Text PDFTumor necrosis factor alpha (TNFα), as a key pro-inflammatory cytokine, plays a central role in joint diseases. In recent years, numerous models of TNFα-induced cartilage inflammation have been developed. However, due to the significant differences between these models and the lack of consensus in their construction, it becomes difficult to compare the results of different studies.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the role of TNFα in inflammatory models for osteoarthritis, focusing on how its effects vary with concentration and chondrocyte growth stages.
- Bovine chondrocytes were used, cultured in a 3D pellet, and exposed to TNFα for 48 hours, revealing that a concentration of 10 ng/mL was required to significantly induce inflammation.
- Results showed that higher passages of chondrocytes (especially passage 3) were more sensitive to TNFα, affecting both gene expression of catabolic/inflammatory markers and protein levels of certain cytokines.