Complete 3D visualization of primate striosomes by KChIP1 immunostaining.

High-resolution 3D reconstruction and morphometric analysis of striosomes was carried out in macaque monkeys by using immunocytochemistry for the Kv4 potassium channel subunit potassium channel interacting protein 1 (KChIP1), a novel marker. The striosomes form a connected reticulum made up of two distinct planar sheets spanning several millimeters in the putamen, and long finger-like branches in the caudate nucleus and putamen. Although their spatial organization is variable, morphometric analysis of the striosomes, utilizing skeletonizations, reveals several quantitative invariant measures of striosome organization, including the following findings: 1) individual bifurcation-free striosome branches are 355 +/- 108.

Evaluation of the mouse model for acute otitis media.

Various animal models have been employed for otitis media research. The mouse has been studied less, in spite of its many advantages. To better understand the suitability of the mouse for studies of otitis media, an evaluation was made of its middle ear inflammatory processes following inoculation with heat-killed Streptococcus pneumoniae (strain 6A), one of the three most common bacteria to cause otitis media in the human.