Publications by authors named "Sarah J Flannery"
CRISPR-Cas nucleases are transforming genome editing, RNA editing, and diagnostics but have been limited to RNA-guided systems. We present ΨDNA, a DNA-based guide for Cas12 enzymes, engineered for specific and efficient RNA targeting. ΨDNA mimics a crRNA but with a reverse orientation, enabling stable Cas12-RNA assembly and activating trans-cleavage without RNA components.
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- * PICNIC operates by separating double-stranded DNA into single-stranded DNA, allowing detection without PAM constraints using various Cas12 enzymes.
- * The method has successfully detected clinically significant mutations, including a drug-resistant HIV-1 variant and HCV variants, demonstrating 100% specificity without relying on PAM sites.